Development and Application of a Validated Stability-Indicating High-Performance Liquid Chromatographic Method Using Photodiode Array Detection for Simultaneous Determination of Granisetron, Methylparaben, Propylparaben, Sodium Benzoate, and Their Main Degradation Products in Oral Pharmaceutical Preparations

2011 ◽  
Vol 94 (5) ◽  
pp. 1447-1460 ◽  
Author(s):  
Ismail Hewala ◽  
Hamed El-Fatatry ◽  
Ehab Emam ◽  
Mokhtar Mabrouk
Keyword(s):  
High Performance ◽  
Degradation Products ◽  
Pharmaceutical Preparations ◽  
High Performance Liquid Chromatographic ◽  
Photodiode Array Detection ◽  
Liquid Chromatographic Method ◽  
Photodiode Array ◽  
Array Detection ◽  
Liquid Chromatographic

Quantitative Determination of Steroid Acetates in Pharmaceutical Preparations by High Performance Liquid Chromatography

1980 ◽  
Vol 63 (6) ◽  
pp. 1184-1188
Author(s):  
Halver C Van Dame
Keyword(s):  
Quantitative Determination ◽  
High Performance ◽  
Degradation Products ◽  
Pharmaceutical Preparations ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Rf Values ◽  
Wavelength Detector ◽  
Liquid Chromatographic

Abstract A high performance liquid chromatographic method is described for the rapid, quantitative determination of corticosteroid acetates in a variety of pharmaceutical preparations. The method not only separates the steroids from other ingredients in tablets, creams, ointments, lotions, or suspensions, but also from their probable degradation products. The steroid acetate is extracted from a tablet matrix or from a suspension with water and acetonitrile (50+50), or from a hexane suspension of a cream, ointment, or lotion with acetonitrile. A liquid chromatogi aph equipped with an accurate flow controller, a variable wavelength detector capable of measurement at 240 nm, and a 10 μL loop injector was used. Components were separated on a μBondpak C18 column with acetonitrile-water as the mobile phase. K’ values of 10 commonly used steroid acetates are shown and compared with Rf values from thin layer chromatographic systems. Results were equivalent for cortisone acetate tablets assayed by both the proposed and the official USP methods. Other pharmaceutical preparations were assayed to test the method, with satisfactory results.

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