scholarly journals Bioprocess Engineering of Pichia pastoris, an Exciting Host Eukaryotic Cell Expression System

10.5772/56407 ◽  
2013 ◽  
Author(s):  
Francisco Valero
Keyword(s):  
Pichia Pastoris ◽  
Expression System ◽  
Eukaryotic Cell ◽  
Bioprocess Engineering ◽  
Cell Expression ◽  
Cell Expression System

Protein production using the baculovirus-insect cell expression system

2013 ◽  
Vol 30 (1) ◽  
pp. 1-18 ◽  
Author(s):  
A. Contreras-Gómez ◽  
A. Sánchez-Mirón ◽  
F. García-Camacho ◽  
E. Molina-Grima ◽  
Y. Chisti
Keyword(s):  
Insect Cell ◽  
Protein Production ◽  
Expression System ◽  
Insect Cell Expression System ◽  
Cell Expression ◽  
Insect Cell Expression ◽  
Cell Expression System

scholarly journals Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression

2008 ◽  
Vol 39 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Luiz Gustavo Bentim Góes ◽  
Antonio Carlos de Freitas ◽  
Oilita Pereira Ferraz ◽  
Tania Tassinari Rieger ◽  
José Ferreira dos Santos ◽  
...  
Keyword(s):  
Protein Expression ◽  
Gene Transfection ◽  
Expression System ◽  
Bovine Papillomavirus ◽  
Cell Expression ◽  
L1 Protein ◽  
Cell Expression System

Activation of recombinant trp by thapsigargin in Sf9 insect cells

1994 ◽  
Vol 267 (5) ◽  
pp. C1501-C1505 ◽  
Author(s):  
L. Vaca ◽  
W. G. Sinkins ◽  
Y. Hu ◽  
D. L. Kunze ◽  
W. P. Schilling
Keyword(s):  
Expression System ◽  
Cation Selectivity ◽  
Drosophila Protein ◽  
Insect Cell Expression System ◽  
Cell Expression ◽  
Insect Cell Expression ◽  
Mammalian Protein ◽  
Entry Mechanism ◽  
Proline Rich Region ◽  
Cell Expression System

The mammalian protein responsible for Ca2+ release-activated current (Icrac) may be homologous to the Drosophila protein designated trp. Thus the activity of trp, and another Drosophila protein designated trp-like or trpl, may be linked to depletion of the internal Ca2+ store via the so-called capacitative Ca2+ entry mechanism. To test this hypothesis, the effect of thapsigargin, a selective inhibitor of the endoplasmic reticulum Ca2+ pump, on trp- and trpl-induced whole cell membrane current was determined using the baculovirus Sf9 insect cell expression system. The results demonstrate that trp and trpl form Ca(2+)-permeable cation channels. The trpl encodes a nonselective cation channel that is constitutively active under basal nonstimulated conditions and is unaffected by thapsigargin, whereas trp is more selective for Ca2+ than Na+ and is activated by depletion of the internal Ca2+ store. Although evaluation of cation selectivity suggests that trp is not identical to the channel responsible for Icrac, these channels must share some structural feature(s) since both are activated by thapsigargin. A unique proline-rich region in the COOH-terminal tail of trp, which is absent in trpl, may be necessary for capacitative Ca2+ entry.

[16] Functional analysis of mutant P450(C21) genes in COS cell expression system

1991 ◽  
pp. 166-173 ◽  
Author(s):  
Yujiro Higashi ◽  
Yoshiaki Fujii-Kuriyama
Keyword(s):  
Functional Analysis ◽  
Expression System ◽  
Cell Expression ◽  
Cell Expression System

scholarly journals Interleukin-4 induces association of the c-fes proto-oncogene product with phosphatidylinositol-3 kinase

Blood ◽  
1996 ◽  
Vol 88 (10) ◽  
pp. 3910-3918 ◽  
Author(s):  
K Izuhara ◽  
RA Feldman ◽  
P Greer ◽  
N Harada
Keyword(s):  
Expression System ◽  
Sh2 Domain ◽  
Pi3 Kinase ◽  
Kinase Assay ◽  
Interleukin 4 ◽  
Related Protein ◽  
Cos7 Cell ◽  
Cell Expression ◽  
Cell Expression System ◽  
Phosphatidylinositol 3

We have previously demonstrated that interleukin-4 (IL-4) induces tyrosine phosphorylation of a protein closely related or identical to the c-fes proto-oncogene product (FES) and association of this protein with the IL-4 receptor alpha chain (IL-4R alpha). IL-4 is known to induce association of phosphatidylinositol-3 (PI3) kinase with the IL-4R alpha. Since FES contains the consensus motifs for PI3 kinase binding, we tested the possibility that FES may associate with PI3 kinase upon IL-4 stimulation. We demonstrate herein that IL-4 stimulation induced rapid association of FES or a related protein with PI3 kinase in mouse T-cell lines. We also show an association of human FES (hFES) with the src homology 2 (SH2) domain of PI3 kinase in a COS7 cell expression system. The in vitro PI3 kinase assay using COS7 cells suggested that hFES partly contributes to the association between the hIL-4R alpha and PI3 kinase. We have further identified the important region in the cytoplasmic domain of the hIL-4R alpha for association of tyrosine-phosphorylated hFES with the hIL-4R alpha and SH2 domain of PI3 kinase using a COS7 cell expression system. These results suggest that FES or a related protein/PI3 kinase pathway may play a role in the pleiotropic effects of IL-4.

scholarly journals Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells

2015 ◽  
Vol 35 (6) ◽  
pp. 1715-1719 ◽  
Author(s):  
PENG CHENG ◽  
SHUYING ZHU ◽  
LI JUN ◽  
LIHUA HUANG ◽  
YAHUI HONG
Keyword(s):  
Cancer Cells ◽  
Insect Cell ◽  
Expression System ◽  
Insect Cell Expression System ◽  
In Vitro Effects ◽  
Cell Expression ◽  
Insect Cell Expression ◽  
Cell Expression System

Segregated characterization of recombinant epoxide hydrolase synthesis via the baculovirus/insect cell expression system

1994 ◽  
Vol 49 (24) ◽  
pp. 4133-4141 ◽  
Author(s):  
William E. Bentley ◽  
Biruk Kebede ◽  
Thomas Franey ◽  
Min-Ying Wang
Keyword(s):  
Insect Cell ◽  
Epoxide Hydrolase ◽  
Expression System ◽  
Insect Cell Expression System ◽  
Cell Expression ◽  
Insect Cell Expression ◽  
Cell Expression System

Deuteration and selective labeling of alanine methyl groups of β2-adrenergic receptor expressed in a baculovirus-insect cell expression system

2018 ◽  
Vol 71 (3) ◽  
pp. 185-192 ◽  
Author(s):  
Yutaka Kofuku ◽  
Tomoki Yokomizo ◽  
Shunsuke Imai ◽  
Yutaro Shiraishi ◽  
Mei Natsume ◽  
...  
Keyword(s):  
Insect Cell ◽  
Adrenergic Receptor ◽  
Expression System ◽  
Methyl Groups ◽  
Selective Labeling ◽  
Β2 Adrenergic Receptor ◽  
Insect Cell Expression System ◽  
Cell Expression ◽  
Insect Cell Expression ◽  
Cell Expression System
Sign in / Sign up

Export Citation Format

Share Document