scholarly journals Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

2017 ◽  
Author(s):  
Lizeth Fuentes-Mera ◽  
Alberto Camacho ◽  
Nidia K. Moncada-Saucedo ◽  
Víctor Peña-Martínez
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue

Spidroin Striped Micropattern Promotes Chondrogenic Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells

2021 ◽  
Author(s):  
Anggraini Barlian ◽  
Dinda Hani’ah Arum Saputri ◽  
Adriel Hernando ◽  
Ekavianty Prajatelistia ◽  
Hutomo Tanoto
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Spider Silk ◽  
Cartilage Tissue Engineering ◽  
Wharton’S Jelly ◽  
Cartilage Tissue ◽  
Type Ii ◽  
Wharton's Jelly

Abstract Cartilage tissue engineering, particularly micropattern, can influence the biophysical properties of mesenchymal stem cells (MSCs) leading to chondrogenesis. In this research, human Wharton’s jelly MSCs (hWJ-MSCs) were grown on a striped micropattern containing spider silk protein (spidroin) from Argiope appensa. This research aims to direct hWJ-MSCs chondrogenesis using micropattern made of spidroin bioink as opposed to fibronectin that often used as the gold standard. Cells were cultured on striped micropattern of 500 µm and 1000 µm width sizes without chondrogenic differentiation medium for 21 days. The immunocytochemistry result showed that spidroin contains RGD sequences and facilitates cell adhesion via integrin β1. Chondrogenesis was observed through the expression of glycosaminoglycan, type II collagen, and SOX9. The result on glycosaminoglycan content proved that 1000 µm was the optimal width to support chondrogenesis. Spidroin micropattern induced significantly higher expression of SOX9 mRNA on day-21 and SOX9 protein was located inside the nucleus starting from day-7. COL2A1 mRNA of spidroin micropattern groups was downregulated on day-21 and collagen type II protein was detected starting from day-14. These results showed that spidroin micropattern enhances chondrogenic markers while maintains long-term upregulation of SOX9, and therefore has the potential as a new method for cartilage tissue engineering.

scholarly journals Cartilage Tissue Engineering Using Mesenchymal Stem Cells and 3D Chitosan Scaffolds – In vitro and in vivo Assays

10.5772/25085 ◽  
2011 ◽  
Author(s):  
Natalia Martins ◽  
Alessandra Arcoverde ◽  
Juliana Lott ◽  
Viviane Silva ◽  
Dawidson Gomes ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
In Vivo Assays ◽  
Chitosan Scaffolds

scholarly journals Mechanical loading inhibits hypertrophy in chondrogenically differentiating hMSCs within a biomimetic hydrogel

2016 ◽  
Vol 4 (20) ◽  
pp. 3562-3574 ◽  
Author(s):  
E. A. Aisenbrey ◽  
S. J. Bryant
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Mechanical Loading ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cartilage Tissue

Three dimensional hydrogels are a promising vehicle for delivery of adult human bone-marrow derived mesenchymal stem cells (hMSCs) for cartilage tissue engineering.

Adipose-derived mesenchymal stem cells for cartilage tissue engineering: State-of-The-Art inin vivostudies

2013 ◽  
Vol 102 (7) ◽  
pp. 2448-2466 ◽  
Author(s):  
Francesca Veronesi ◽  
Melania Maglio ◽  
Matilde Tschon ◽  
Nicolò Nicoli Aldini ◽  
Milena Fini
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
State Of The Art ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Engineering State

Chondrogenesis of human umbilical cord-derived mesenchymal stem cells in silk-fibroin/hyaluronic acid scaffolds for cartilage tissue engineering

2014 ◽  
Vol 185 ◽  
pp. S125 ◽  
Author(s):  
Jirayut Jaipaew ◽  
Piyanun Wangkulangkul ◽  
Chitkasem Suwanrath ◽  
Jirut Meesane ◽  
Supaporn Krivimol ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Umbilical Cord ◽  
Silk Fibroin ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Human Umbilical Cord
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