Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification

The adoption of biocatalysis in solvent-free systems is an alternative to establish a greener esters production. An interesting correlation between the acid:alcohol molar ratio and biocatalyst (immobilized lipase) loading in the optimization of ester syntheses in solvent-free systems had been observed and explored. A simple mathematical tool named Substrate-Enzyme Relation (SER) has been developed, indicating a range of reaction conditions that resulted in high conversions. Here, SER utility has been validated using data from the literature and experimental assays, totalizing 39 different examples of solvent-free enzymatic esterifications. We found a good correlation between the SER trends and reaction conditions that promoted high conversions on the syntheses of short, mid, or long-chain esters. Moreover, the predictions obtained with SER are coherent with thermodynamic and kinetics aspects of enzymatic esterification in solvent-free systems. SER is an easy-to-handle tool to predict the reaction behavior, allowing obtaining optimum reaction conditions with a reduced number of experiments, including the adoption of reduced biocatalysts loadings.

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Solvent-Free Production of Ethylene Glycol Monostearate through Enzymatic Esterification

Industrial & Engineering Chemistry Research ◽

10.1021/acs.iecr.7b05365 ◽

2018 ◽

Vol 57 (19) ◽

pp. 6627-6632 ◽

Cited By ~ 4

Author(s):

Gabriela N. Pereira ◽

Jefferson P. Holz ◽

Lindomar Lerin ◽

Mara Cristina Picoli Zenevicz ◽

Débora de Oliveira ◽

...

Keyword(s):

Ethylene Glycol ◽

Solvent Free ◽

Enzymatic Esterification

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Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2005.08.011 ◽

2006 ◽

Vol 38 (6) ◽

pp. 788-794 ◽

Cited By ~ 46

Author(s):

Hanen Ghamgui ◽

Maha Karra-Chaâbouni ◽

Sofiane Bezzine ◽

Nabil Miled ◽

Youssef Gargouri

Keyword(s):

Isoamyl Acetate ◽

Solvent Free ◽

Free System ◽

Solvent Free System ◽

Staphylococcus Simulans

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Applicability of CoA/acetyl-CoA manipulation system to enhance isoamyl acetate production in Escherichia coli

Metabolic Engineering ◽

10.1016/j.ymben.2004.02.006 ◽

2004 ◽

Vol 6 (4) ◽

pp. 294-299 ◽

Cited By ~ 41

Author(s):

Ravishankar V. Vadali ◽

George N. Bennett ◽

Ka-Yiu San

Keyword(s):

Escherichia Coli ◽

Isoamyl Acetate ◽

Acetate Production ◽

Acetyl Coa ◽

Manipulation System

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Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase

Chemical and Biochemical Engineering Quarterly ◽

10.15255/cabeq.2019.1692 ◽

2020 ◽

Vol 33 (4) ◽

pp. 495-499

Author(s):

M. Matsumoto ◽

Y. Hasegawa

Keyword(s):

Carboxylic Acids ◽

Propionic Acid ◽

Catalytic Reaction ◽

Reaction Rate ◽

Isoamyl Alcohol ◽

Reaction Rate Constant ◽

Solvent Free ◽

Enzymatic Esterification ◽

Catalytic Activities ◽

Kinetics Of

To avoid the use of compounds that burden the environment, a solvent-free enzymatic<br /> reaction was the focus of this study. Investigated were the catalytic activities and kinetics of lipases that were pretreated with carboxylic acids for the solvent-free esterification of propionic acid with isoamyl alcohol. The enhancements of the esterification yields and rates by the bio-imprinting effects of carboxylic acids were observed. We found no inhibition of isoamyl alcohol on the solvent-free enzymatic esterification, and obtained a large imprinting effect under a largely excessive amount of isoamyl alcohol to propionic acid. From the kinetic analysis, the imprinting of lipases mainly enhanced the catalytic reaction rate constant rather than the affinity between lipase and propionic acid compared with untreated lipase. The bio-imprinting treatment of lipase is found to be very effective for the yield and kinetics in solvent-free esterification.

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