scholarly journals Advanced Biocrystallogenesis

2021 ◽  
Author(s):  
Ivana Kuta Smatanova ◽  
Petra Havlickova ◽  
Barbora Kascakova ◽  
Tatyana Prudnikova
Keyword(s):  
Biochemical Parameters ◽  
Microgravity Condition ◽  
Living Cells ◽  
Microfluidic Chips ◽  
X Ray Crystallography ◽  
Protein Sample ◽  
Electric And Magnetic Fields ◽  
Advanced Crystallization ◽  
Macromolecular Crystallization

Nowadays, X-ray crystallography is one of the most popular structural biology methods. Successful crystallization depends not only on the quality of the protein sample, precipitant composition, pH or other biophysical and biochemical parameters, but also largely on the use of crystallization technique. Some proteins are difficult to be crystallized using basic crystallization methods; therefore, several advanced methods for macromolecular crystallization have been developed. This chapter briefly reviews the most promising advanced crystallization techniques and strategies as one of the efficient tools for crystallization of macromolecules. Crystallization in capillaries, gels, microfluidic chips, electric and magnetic fields as well as crystallization under microgravity condition and crystallization in living cells are briefly described.

The impact of sweet basil plant additive on biochemical parameters and organoleptic quality of meat of chicken

2020 ◽  
Vol 23 (07) ◽  
pp. 254-260
Author(s):  
Thair Jawad Kadhim Al-Kelabi ◽  
Mayada F. Mohamed ◽  
Hassan Al-Karagoly
Keyword(s):  
Biochemical Parameters ◽  
Organoleptic Quality ◽  
Sweet Basil ◽  
The Impact

scholarly journals Predictors of Quality of Life in Acromegaly: No Consensus on Biochemical Parameters

2017 ◽  
Vol 8 ◽  
Author(s):  
Victor J. Geraedts ◽  
Cornelie D. Andela ◽  
Günter K. Stalla ◽  
Alberto M. Pereira ◽  
Wouter R. van Furth ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Biochemical Parameters

Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Deepshika Gilbile ◽  
Megan L. Shelby ◽  
Artem Y. Lyubimov ◽  
Jennifer L. Wierman ◽  
Diana C. F. Monteiro ◽  
...  
Keyword(s):  
Room Temperature ◽  
Microfluidic Chips ◽  
Fixed Target ◽  
Plug And Play ◽  
X Ray ◽  
X Ray Crystallography ◽  
Serial Crystallography

This work presents our development of versatile, inexpensive, and robust polymer microfluidic chips for routine and reliable room temperature serial X-ray crystallography measurements.

scholarly journals Correlations Between Clinical Parameters and Health-Related Quality of Life in Postmenopausal Osteoporotic Women

2016 ◽  
Vol 17 (4) ◽  
pp. 333-342
Author(s):  
Vesna Grbovic ◽  
Aleksandra Jurisic Skevin ◽  
Katarina Parezanovic Ilic ◽  
Aleksandra Lucic Tomic ◽  
Jasmin Nurkovic ◽  
...  
Keyword(s):  
Femoral Neck ◽  
Biochemical Parameters ◽  
Health Perception ◽  
Mental Function ◽  
Health Related Quality ◽  
T Score ◽  
Related Quality ◽  
Health Related ◽  
Fracture History

Abstract The purpose of this study was to assess the correlation between health-related quality of life (HRQoL) and clinically relevant osteodensitometric and biochemical parameters in postmenopausal osteoporotic women. Bone mineral density (BMD) and T scores of the lumbar vertebrae and femoral neck were assessed in 100 osteoporotic women (56 without previous fractures and 44 with previous fractures) using dual x-ray absorptiometry. The Fracture Risk Assessment Tool (FRAX) index for major osteoporotic and hip fractures was calculated based on demographic data and hip BMD. Venous blood samples were taken from each subject for biochemical analysis (serum calcium, phosphorus, alkaline phosphatase and vitamin D levels). HRQoL was assessed using the QUALEFFO-41 questionnaire (domains: Health perception, Pain, and Physical, Social and Mental function). Basic participant characteristics (age, menopause length, body mass index, smoking habits, hereditary tendency towards fracture, fracture history) correlated with some of the QUALEFFO-41 domains, but the correlation coefficients were low (r<0.3), except in the case of the correlation between Pain and fracture history (r=0.638). Of the six variables included in the multiple regression model, fracture history was shown to be the most significant predictor with respect to the following three QUALEFFO-41 domains: Pain (b=20.511), Social function (b=2.548) and Health perception (b=3.185). Correlation analysis showed that after adjustment for basic characteristics, BMD and T score of the femoral neck and Pain (r=0.331 and r=0.449, respectively), Social function (r=0.422 and r=0.419) and Health perception (r=0.434 for T score of the femoral neck) exhibited the strongest correlations. Vitamin D was negatively correlated with Mental function, while the other biochemical parameters exhibited variable correlations with the QUALEFFO- 41 domains (r≈0.2-0.5). Our study confirmed the previously established relationship between BMD of the femoral neck and HRQoL in patients with osteoporosis and demonstrated correlations between various blood bone metabolism parameters and HRQoL that have not been previously investigated.

scholarly journals Clinical, Biochemical and Haemodynamic Effects of the Intrathecal Ketamine for Ovariohysterectomy in Bitches

2012 ◽  
Vol 56 (3) ◽  
pp. 299-303 ◽  
Author(s):  
Sadik Yayla ◽  
Cihan Kacar ◽  
Duygu Kaya ◽  
Oguz Merhan ◽  
Ozgur Aksoy ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Biochemical Parameters ◽  
Haemodynamic Effects ◽  
Spinal Needle ◽  
Depth Of Anaesthesia ◽  
Depressive Effect ◽  
Haemodynamic Parameters ◽  
Cardiac Functions ◽  
Ketamine Hcl

Abstract The purpose of the study was to evaluate the effects of intrathecal (IT) ketamine HCl anesthesia on clinical values and some haemodynamic and biochemical parameters in bitches. An IT ketamine (10 mg/kg) was administered to 30 bitches with a spinal needle (18-22 G) in the lumbosacral space. The haemodynamic parameters were monitored and some biochemical values were assessed (blood gase, oxygen-haemoglobin, and electrolyte levels). The length and depth of anaesthesia was determined with a pinprick test, touching to the ligamenta lata uteri and incision. Anaesthesia took effect in less than 1 min in all dogs and has lasted an average of 95.9 min. In spite of the fact that the dogs recovered completely from the effects of dissociative anaesthesia, the anaesthesia in the some extremities was observed to be continued for an average of 17 min longer. The use of IT ketamine HCl raised blood pressure and did not have a depressive effect on respiratory and cardiac functions. It was concluded that ketamine HCl could be an appropriate alternative for ovariohysterectomy operations in bitches when the quality of the anaesthesia and the prevention of bradycardia and hypotension are considered.

scholarly journals Biochemical parameters and its association with quality of life in patients with diabetic foot ulcers

2019 ◽  
Vol 10 (1) ◽  
pp. 16-23
Author(s):  
Hiren Sanghani ◽  
◽  
Ashish kumar Agravatt ◽  
Heeya Shah ◽  
Niyati Lakhani ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Diabetic Foot ◽  
Biochemical Parameters ◽  
Foot Ulcers ◽  
Diabetic Foot Ulcers

scholarly journals A comparative anatomy of protein crystals: lessons from the automatic processing of 56,000 samples

2019 ◽  
Author(s):  
Olof Svensson ◽  
Maciej Gilski ◽  
Didier Nurizzo ◽  
Matthew W. Bowler
Keyword(s):  
Molecular Weight ◽  
Data Collection ◽  
Comparative Anatomy ◽  
Automatic Processing ◽  
Data Set ◽  
X Ray Crystallography ◽  
Cell Dimensions ◽  
Unit Cell Dimensions ◽  
Crystal Volume

SynopsisThe automatic processing of over 56,000 crystals by the autonomous ESRF beamline MASSIF-1 has provided a data set of crystal characteristics and properties that allows many theoretical proposals and assumptions to be evaluated experimentally.AbstractThe fully automatic processing of crystals of macromolecules has presented a unique opportunity to gather information on the samples that is not usually recorded. This has proved invaluable in improving the sample location, characterisation and data collection algorithms. After operating for four years, MASSIF-1 has now processed over 56,000 samples, gathering information at each stage, from the volume of the crystal to the unit cell dimensions, space group, quality of the data collected and the reasoning behind the decisions made in data collection. This provides an unprecedented opportunity to analyse these data together, providing a detailed landscape of macromolecular crystals and intimate details of their contents and, importantly, how the two are related. The data show that mosaic spread is unrelated to the size or shape of crystals and demonstrate experimentally that diffraction intensities scale in proportion to crystal volume and molecular weight. It is also shown that crystal volume scales inversely with molecular weight. The results set the scene for the development of X-ray crystallography in a changing environment for structural biology.

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