scholarly journals Identification of Meat Species Using Species-Specific PCR-RFLP Fingerprint of Mitochondrial 12S rRNA Gene

2007 ◽  
Vol 27 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Jong-Keun Park ◽  
Ki-Hyun Shin ◽  
Sung-Chul Shin ◽  
Ku-Young Chung ◽  
Eui-Ryong Chung
Keyword(s):  
12S Rrna ◽  
Rrna Gene ◽  
Specific Pcr ◽  
12S Rrna Gene ◽  
Pcr Rflp ◽  
Meat Species ◽  
Mitochondrial 12S Rrna ◽  
Species Specific

PCR Identification of Ruminant Tissue in Raw and Heat-Treated Meat Meals

2006 ◽  
Vol 69 (9) ◽  
pp. 2241-2247 ◽  
Author(s):  
JEONG CHUL HA ◽  
WAN TAE JUNG ◽  
YONG SUK NAM ◽  
TAE WHA MOON
Keyword(s):  
Dna Sequence ◽  
12S Rrna ◽  
Spongiform Encephalopathy ◽  
Rrna Gene ◽  
Specific Pcr ◽  
Pcr Product ◽  
Heat Treated ◽  
Mitochondrial 12S Rrna ◽  
Animal Feedstuffs ◽  
Species Specific

To control the spread of bovine spongiform encephalopathy in cattle through contaminated animal feedstuffs, screening of feed products is essential. We designed five pairs of primers to identify specifically raw and heat-treated tissue from cattle, sheep, goat, deer, and ruminants in general. A forward common primer was designed based on a conserved DNA sequence in the mitochondrial 12S rRNA–tRNAval–16S rRNA gene, and reverse primers were designed to hybridize with a species-specific DNA sequence for each species considered. All primers were developed to create a specific PCR product small enough (less than 200 bp) to be suitable for heat-treated material. To evaluate the effect of heat treatment, a severe sterilization condition (133°C at 300 kPa for 20 min) was chosen. Species-specific amplicons were obtained from all types of heat-treated meat meals. Analysis of laboratory-contaminated vegetable meals revealed that the detection limit of the assay was 0.05% for each species analyzed. This PCR-based analysis can be used as a routine method for detecting banned animal-derived ingredients in raw and heat-treated feedstuffs.

scholarly journals Identification of commercial meats from Amazonas, Peru using PCR-RFLP of mitochondrial 12S rRNA gene

2020 ◽  
Vol 23 ◽  
Author(s):  
Josué Tafur-Culqui ◽  
Martha S. Calderon ◽  
Danilo E. Bustamante
Keyword(s):  
Meat Products ◽  
Restriction Enzymes ◽  
12S Rrna ◽  
Processed Meat ◽  
Rrna Gene ◽  
As Species ◽  
12S Rrna Gene ◽  
Pcr Rflp ◽  
Commercial Species ◽  
Mitochondrial 12S Rrna

Abstract The use of analytical methodologies based on DNA (e.g. PCR-RFLP) to determine the authenticity of different types of meat products after the initial labeling is pivotal to avoid fraudulent practices due to the increased rates of meat consumption. Our PCR-RFLP of mitochondrial 12S rRNA gene using restriction enzymes (AluI and ApoI) aimed to confirm the accuracy of the meat species labeling, based on fresh and processed meat collected in central markets along the main cities in the Amazonas Region (Bagua, Bagua Grande, Chachapoyas, Luya, Pedro Ruiz, Rodriguez de Mendoza). Our analyses qualitatively identified and differentiated three commercial species of fresh meat (bovine, porcine, ovine) and also found the substitution of goat by sheep meat. Regarding processed meat, its composition was uncertain and further analyses should be addressed to determine the meat origin. Monitoring using DNA-based analytical methods of meat trade is suggested to determine fraudulent practices, such as species substitution in markets along regions of Peru.

Sequence analysis of mitochondrial 12S rRNA gene can identify meat species

Meat Science ◽  
2004 ◽  
Vol 66 (3) ◽  
pp. 551-556 ◽  
Author(s):  
P.S. Girish ◽  
A.S.R. Anjaneyulu ◽  
K.N. Viswas ◽  
M. Anand ◽  
N. Rajkumar ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
12S Rrna ◽  
Rrna Gene ◽  
12S Rrna Gene ◽  
Meat Species ◽  
Mitochondrial 12S Rrna

scholarly journals Sequence Characterization of Mitochondrial 12S rRNA Gene in Mouse Deer (Moschiola indica) for PCR-RFLP Based Species Identification

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Chandra Mohan Siddappa ◽  
Mohini Saini ◽  
Asit Das ◽  
Ramesh Doreswamy ◽  
Anil K. Sharma ◽  
...  
Keyword(s):  
Species Identification ◽  
Genomic Dna ◽  
Gene Sequence ◽  
12S Rrna ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Deer Species ◽  
12S Rrna Gene ◽  
Pcr Rflp ◽  
Mitochondrial 12S Rrna

Mitochondrial 12S rRNA has proven to be a useful molecular marker for better conservation and management of the endangered species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the mitochondrial 12S rRNA gene has proven to be a reliable and efficient tool for the identification of different Indian deer species of family cervidae. In the present study, mitochondrial 12S rRNA gene sequence of mouse deer (Moschiola indica) belonging to the family Tragulidae was characterized and analysed in silico for its use in species identification. Genomic DNA was isolated from the hair follicles and mitochondrial 12S rRNA gene was amplified using universal primers. PCR product was cloned and sequenced for the first time. The sequence of mouse deer showed 90.04, 90.08, 90.04, 91.2, 90.04, and 90.08% identities with sika deer, sambar, hog deer, musk deer, chital, and barking deer, respectively. Restriction mapping in Lasergene (DNAstar Inc., Madison, WI, USA) revealed that mouse deer mitochondrial 12S rRNA gene sequence can be differentiated from the other deer species in PCR-RFLP using RsaI, DdeI, BsrI, and BstSFI. With the help of predicted pattern, mouse deer can be identified using genomic DNA from a variety of biomaterials, thereby providing molecular aid in wildlife forensics and conservation of the species.

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