Polymerase Chain Reaction–Restriction Fragment Length Polymorphism of Mitochondrial 12S rRNA Gene: A Simple Method for Identification of Poultry Meat Species

2007 ◽  
Vol 31 (4) ◽  
pp. 447-455 ◽  
Author(s):  
P. S. Girish ◽  
A. S. R. Anjaneyulu ◽  
K. N. Viswas ◽  
F. H. Santhosh ◽  
K. N. Bhilegaonkar ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Poultry Meat ◽  
12S Rrna ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Simple Method ◽  
12S Rrna Gene ◽  
Meat Species ◽  
Mitochondrial 12S Rrna ◽  
Polymerase Chain

scholarly journals Identification of Meat Species Using Species-Specific PCR-RFLP Fingerprint of Mitochondrial 12S rRNA Gene

2007 ◽  
Vol 27 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Jong-Keun Park ◽  
Ki-Hyun Shin ◽  
Sung-Chul Shin ◽  
Ku-Young Chung ◽  
Eui-Ryong Chung
Keyword(s):  
12S Rrna ◽  
Rrna Gene ◽  
Specific Pcr ◽  
12S Rrna Gene ◽  
Pcr Rflp ◽  
Meat Species ◽  
Mitochondrial 12S Rrna ◽  
Species Specific

Sequence analysis of mitochondrial 12S rRNA gene can identify meat species

Meat Science ◽  
2004 ◽  
Vol 66 (3) ◽  
pp. 551-556 ◽  
Author(s):  
P.S. Girish ◽  
A.S.R. Anjaneyulu ◽  
K.N. Viswas ◽  
M. Anand ◽  
N. Rajkumar ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
12S Rrna ◽  
Rrna Gene ◽  
12S Rrna Gene ◽  
Meat Species ◽  
Mitochondrial 12S Rrna

Identification of Nile Perch (Lates niloticus), Grouper (Epinephelus guaza), and Wreck Fish (Polyprion americanus) by Polymerase Chain Reaction–Restriction Fragment Length Polymorphism of a 12S rRNA Gene Fragment

2000 ◽  
Vol 63 (9) ◽  
pp. 1248-1252 ◽  
Author(s):  
LUIS ASENSIO ◽  
ISABEL GONZÁLEZ ◽  
ALICIA FERNÁNDEZ ◽  
ANA CÉSPEDES ◽  
PABLO E. HERNÁNDEZ ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
12S Rrna ◽  
Rrna Gene ◽  
Nile Perch ◽  
Chain Reaction ◽  
12S Rrna Gene ◽  
Lates Niloticus ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Polyprion Americanus

Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the 12S rRNA gene has been used for the specific identification of Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus). Amplification of DNA isolated from muscle samples was carried out using a set of primers flanking a region of 436 bp from the mitochondrial 12S rRNA gene. Digestions of the PCR products with RsaI and Sau96I endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of each species analyzed.

scholarly journals Phytoplasma occurrence in apple trees in the Czech Republic

2011 ◽  
Vol 39 (No. 1) ◽  
pp. 7-12 ◽  
Author(s):  
R. Fialová ◽  
M. Navrátil ◽  
P. Válová
Keyword(s):  
Fragment Length Polymorphism ◽  
Rrna Gene ◽  
Polymorphism Analysis ◽  
Apple Proliferation ◽  
Chain Reaction ◽  
Apple Trees ◽  
The Czech Republic ◽  
High Incidence ◽  
Phytoplasma Infection ◽  
Polymerase Chain

The presence of phytoplasmas in apple trees with proliferation symptoms, rubbery wood symptoms and no symp­toms was determined by using polymerase chain reaction assays with primers amplifying phytoplasma 16S rRNA gene. Phytoplasmas were detected in all trees with proliferation symptoms. Positive tests for phytoplasma in the group of trees with rubbery wood symptoms and of those without symptoms revealed a relatively high incidence of latent phytoplasma infection. Using restriction fragment length polymorphism analysis, phytoplasma of the same identity – apple proliferation phytoplasma (subgroup 16SrX-A) – was recorded in all positively tested trees.  

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