Validation of p53 Immunohistochemistry as a Prognostic Factor in Breast Cancer in Clinical Practice

Abstract Context.—Abnormal p53 tumor suppressor gene expression as detected by immunohistochemistry is a possible prognostic factor in breast cancer. The difference in techniques used to evaluate the expression of mutated p53 protein is under intense scrutiny, as well as its uses either independently or in conjunction with other prognostic factors in breast cancer. Objective.—To determine whether p53 immunohistochemistry can be used as a reliable indicator of the presence of mutated nuclear p53 protein, and whether this method can be performed reliably in a community hospital's clinical practice. Design.—One hundred twenty-two cases of breast carcinoma were stained and analyzed for the presence of p53 protein using DO-7 (Dako Corporation, Carpinteria, Calif) p53 antibody. Results.—Of the 122 cases of invasive carcinoma studied, 23 (18.7%) were positive for p53, and 16 (16.3%) of 98 cases with coexisting ductal carcinoma in situ were positive for p53. This finding is in agreement with comparable published studies. Conclusions.—Based on the results of this study, we conclude that p53 immunohistochemistry qualifies as a diagnostic technique suitable for clinical practice in a community hospital. Its detection may be particularly promising in clinical trials of new molecular therapies directed at the p53 tumor suppressor gene.

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The p53 tumor-suppressor gene in human breast cancer

Cancer Treatment and Research - Mammary Tumorigenesis and Malignant Progression ◽

10.1007/978-1-4615-2592-9_4 ◽

1994 ◽

pp. 63-77 ◽

Cited By ~ 11

Author(s):

D. Craig Allred ◽

Richard Elledge ◽

Gary M. Clark ◽

Suzanne A. W. Fuqua

Keyword(s):

Breast Cancer ◽

Tumor Suppressor ◽

Tumor Suppressor Gene ◽

Human Breast Cancer ◽

Suppressor Gene ◽

Human Breast ◽

P53 Tumor Suppressor ◽

P53 Tumor Suppressor Gene

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Spectrum of p53 Tumor Suppressor Gene Mutations and Breast Cancer Survival

Breast Cancer Research and Treatment ◽

10.1023/b:brea.0000010699.53742.60 ◽

2004 ◽

Vol 83 (1) ◽

pp. 57-66 ◽

Cited By ~ 18

Author(s):

Hong Lai ◽

Fangchao Ma ◽

Edward Trapido ◽

Lou Meng ◽

Shenghan Lai

Keyword(s):

Breast Cancer ◽

Tumor Suppressor ◽

Tumor Suppressor Gene ◽

Cancer Survival ◽

Gene Mutations ◽

Suppressor Gene ◽

Breast Cancer Survival ◽

P53 Tumor Suppressor ◽

P53 Tumor Suppressor Gene

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Expression of p53 Protein in Pterygium

European Journal of Ophthalmology ◽

10.1177/112067219800800307 ◽

1998 ◽

Vol 8 (3) ◽

pp. 157-161 ◽

Cited By ~ 17

Author(s):

C. Onur ◽

D. Orhan ◽

M. Orhan ◽

S. Dizbay Sak ◽

Ö. Tulunay ◽

...

Keyword(s):

Tumor Suppressor ◽

Tumor Suppressor Gene ◽

Uv Radiation ◽

P53 Protein ◽

Suppressor Gene ◽

Tumor Formation ◽

Nuclear Staining ◽

P53 Tumor Suppressor ◽

P53 Tumor Suppressor Gene

Purpose The pathogenesis of pterygium is still not completely understood and many environmental factors, including ultraviolet (UV) radiation, play an important role in its etiology. Chronic exposure to UV radiation causes mutations in the p53 tumor suppressor gene, eventually leading to tumor formation. We analyzed the immunohistochemical expression of p53 proteins in pterygial tissues to determine the role of the p53 tumor suppressor gene in the development of pterygium. Methods Pterygial specimens were studied immunohistochemically using antibodies against p53 protein. Results Out of 38 specimens studied, 35 (92.1%) had conjunctival epithelial cells without p53 specific nuclear staining. Only three specimens (7.9%) had a few p53 stained cells. The role of UV radiation in the pathogenesis of pterygium is supported by epidemiological, geographical and microscopic findings. However, our results are not consistent with these data on a genetic basis. Conclusions We conclude that defective p53 tumor suppressor gene function seems to have no role in the pathogenesis of pterygium.

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Immunohistochemical Detection of p53 Tumor Suppressor Gene Protein in Canine Epithelial Colorectal Tumors

Veterinary Pathology ◽

10.1177/030098589703400503 ◽

1997 ◽

Vol 34 (5) ◽

pp. 394-404 ◽

Cited By ~ 34

Author(s):

J. C. Wolf ◽

P. E. Ginn ◽

B. Homer ◽

L. E. Fox ◽

I. D. Kurzman

Keyword(s):

Tumor Suppressor ◽

Survival Time ◽

Tumor Suppressor Gene ◽

Survival Data ◽

P53 Protein ◽

Suppressor Gene ◽

Positive Staining ◽

Colorectal Tumors ◽

P53 Tumor Suppressor ◽

P53 Tumor Suppressor Gene

Eighty canine epithelial colorectal tumors obtained by excisional biopsy were evaluated immunohistochemically for p53 tumor suppressor gene protein. Dogs in the study averaged 6.9 years of age (range, 1-12.5 years). A standard avidin-biotin immunohistochemical protocol incorporated a polyclonal antibody of rabbit origin (CM-1) as the primary antibody. Positive staining was observed within all subcategories of lesions, including hyperplastic polyps 1/2 (50%), adenomas 14/29 (48%), carcinomas in situ 9/22 (41%), adenocarcinomas 3/4 (75%), and invasive carcinomas 8/23 (35%). A total of 35/80 (44%) positive tumors were identified. Fifteen of 31 (48%) benign tumors labeled for p53 protein compared to 20/49 (41%) malignant tumors. Survival data was available for 57/80 (71%) dogs. The average age of dogs within the group with survival data was 4.4 years. Males predominated 34/57 (60%). Mean survival time was 20.6 months. There was no significant difference in survival time between dogs grouped according to p53 immunoreactivity, cellular stain location, or tumor site. A statistically significant increase in survival time was observed between dogs with clean surgical margins and those without ( P < 0.018) and for dogs with adenomas or carcinomas in situ over dogs with invasive carcinomas ( P < 0.02). In this study, the overall greater positive staining frequency of benign lesions compared to malignant lesions is contrary to data derived from similar immunohistochemical analyses of human tumors and is incongruous with the theorized late-stage participation of the p53 protein in the development of human colorectal cancers. The results of this study suggest that if the p53 tumor suppressor gene protein is involved in the progression of canine colorectal tumors, it may play a relatively early role, possibly analogous to the early appearance of p53 overexpression in precancerous lesions of human ulcerative colitis. Immunohistochemical detection of p53 was not useful prognostically.

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