scholarly journals In vitro study of antiamoebic activity of methanol extract of fruit of Pimpinella anisum on trophozoites of Entamoeba histolytica HM1-IMSS

2013 ◽  
Vol 12 (16) ◽  
pp. 2065-2068
Author(s):  
Y Quintilde ones Gutieacute rrez ◽  
M J Verde Star ◽  
C Rivas Morales ◽  
Or ◽  
A ay Caacute rdenas ◽  
...  
2010 ◽  
Vol 5 (1) ◽  
Author(s):  
Sujit Kumar Sarker ◽  
Nilufar Begum ◽  
Dinesh Mondal ◽  
Md. Abdullah Siddique ◽  
Mohammad A. Rashid

Author(s):  
Joel H. Elizondo-Luévano ◽  
Rocío Castro-Ríos ◽  
Eduardo Sánchez-García ◽  
Magda E. Hernández-García ◽  
Javier Vargas-Villarreal ◽  
...  

Infections caused by parasites in humans represent one of the main public health concerns. Amoebiasis, a parasitic infection caused byEntamoeba histolytica(E. histolytica), is considered endemic in Mexico, whereArgemone mexicana(A. mexicana) has been used in traditional medicine to treat intestinal parasitic diseases. The objective of this work was to evaluate the potential biological activity ofA. mexicanaonE. histolytica. For this purpose, a methanolic extract was prepared fromA. mexicanaleaves, and a differential fractionation was carried out with solvents of different polarities. The inhibitory capacities of the extract and its fractions were evaluated in vitro using HM1-IMSS, a strain ofEntamoeba histolytica.A. mexicanaextract was found to have a growth-inhibiting activity forE. histolytica, showing IC50 = 78.39 μg/mL. The extract was characterized phytochemically, and the methanolic extract fractions were analyzed by liquid chromatography (HPLC) and mass spectrometry (MS). Berberine and jatrorrhizine were present in the active fractions, and these compounds may be responsible for the antiparasitic activity. The identification of amoebicidal activity ofA. mexicanaonE. histolyticagives support to the traditional use. Further studies with berberine and jatrorrhizine will be carried out to understand the mechanism involved.


2018 ◽  
Vol 29 (21) ◽  
Author(s):  
Ogugua Victor Nwadiogbu ◽  
Njoku OU ◽  
Egba Simeon Ikechukw ◽  
Robert Ikechukw Uroko ◽  
Ignatius Glory

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