Piper multinodum C.DC. (Piperaceae) essential oils chemical variation and biological activity against Mycobacterium tuberculosis

Background: Microbial resistance to antibiotics and their adverse effects related to these antibiotics are a matter of global public health in the 21th century. The emergence of drug-resistant strains, has gained the interest of the scientists to discover new antimicrobial agents from the essential oil of medicinal plants. Methods: Anti-mycobacterial effects of Trachyspermum copticum and Pelargonium graveolens essential oils were determined against multi-drug resistant clinical strains of Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium fortuitum and standard strain of Mycobacterium tuberculosis H37Rv by a Broth micro-dilution method. Pelargonium graveolens plant named Narmada was discovered by Kulkarni R.N et al. (Patent ID, USPP12425P2) and a formulation comprising thymol obtained from Trachyspermum is useful in the treatment of drug-resistant bacterial infections (Patent ID, US6824795B2). The chemical composition of hydro-distilled essential oils was determined by GC and GC-MS. Results: Minimum Inhibitory Concentration (MIC) values for T. copticum essential oil against tested isolates were ranged from 19.5 µg/mL to 78 µg/mL. The least minimum inhibitory concentration of P. graveolens extract against M. Kansasii and MDR-TB was 78 µg/ml. Conclusion: The results of the present research introduced T. copticum and P. graveolens essential oils as a remarkable natural anti-mycobacterial agent, but more pharmacological studies are required to evaluate their efficacy in animal models.

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Methylation of Acridin-9-ylthioureas. Structure, Fluorescence and Biological Properties of Products

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20040833 ◽

2004 ◽

Vol 69 (4) ◽

pp. 833-849 ◽

Cited By ~ 15

Author(s):

Juraj Bernát ◽

Eva Balentová ◽

Pavol Kristian ◽

Ján Imrich ◽

Erik Sedlák ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Biological Activity ◽

Ab Initio ◽

Quantum Chemical ◽

Biological Properties ◽

Reaction Conditions ◽

Alkyl Aryl ◽

Nmr Techniques ◽

Primary Amino ◽

Secondary Amino

The structure, fluorescence, biological properties and S(N)-methylation reactions of ten 1,1-alkyl/aryl-disubstituted 3-(acridin-9-yl)thioureas 4 have been studied. Various reaction conditions allowed to obtain corresponding S-methyl 5 and S,N-dimethyl derivatives 6 in good yields. Structure and stereochemistry of the synthesized products are demonstrated by ab initio quantum chemical calculations and NMR techniques including PDQF-COSY, selective INEPT and NOE-difference experiments. Remarkable upfield 13C shifts of resonance signals of carbons C-4a, C-10a adjacent to acridine N-10 are characteristic of hydroiodides in contrast to free bases. Z configuration in isothioureas 7 with secondary amino rest in relation to E configuration of isothioureas with primary amino rest is discussed. Of the obtained products, some isothiourea salts 6 exhibit more than 2 orders of magnitude higher intensity of fluorescence, using 9-isothiocyanatoacridine as a standard. The obtained isothiourea hydroiodides 5 and dimethylisothiourea iodides 6 show remarkable biological activity against Mycobacterium tuberculosis.

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Factors affecting the immunizing activity of ribosomal fractions isolated from Mycobacterium tuberculosis var. bovis strain BCG

Canadian Journal of Microbiology ◽

10.1139/m75-221 ◽

1975 ◽

Vol 21 (10) ◽

pp. 1492-1499 ◽

Cited By ~ 2

Author(s):

V. Portelance ◽

R. Brasseur ◽

R. P. Boulanger

Keyword(s):

Mycobacterium Tuberculosis ◽

Biological Activity ◽

Dose Level ◽

Column Chromatography ◽

Bcg Vaccine ◽

Factors Affecting ◽

H37rv Strain ◽

High Level

Levels of antituberculous immunity similar to those induced by live BCG vaccine were detected in CF1 mice immunized with ribosomal fractions isolated from Mycobacterium tuberculosis var. bovis, strain BCG, and challenged 3 weeks later with the virulent H37Rv strain of Mycobacterium tuberculosis var. hominis. The activity of the crude ribosomal preparations was found to be a function of the immunizing doses and the immunity induced by 1.0-mg doses remained at the same high level after 4 weeks of storage at 4 °C but decreased markedly thereafter. Dialysis and lyophilization had no detrimental effects on the activity of the crude preparations whereas purification by column chromatography on Sephadex G-200 annihilated their biological activity. Crude low-polysaccharide-containing preparations were found inactive even at the 1.0-mg dose level and results of experiments performed with crude ribosomal fractions of varying polysaccharide contents strongly suggest that polysaccharides, or RNA-polysaccharide complexes, may play an important role in the induction of immunity with crude ribosomal fractions isolated from the BCG strain of Mycobacterium tuberculosis var. bovis.

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