scholarly journals Is the creatine kinase isoenzyme MB level a marker of myocardial ischemia in ventilated premature infants?

2016 ◽  
Vol 52 (4) ◽  
Author(s):  
Adauto D. M. Barbosa ◽  
Maria Paula S. Goldani ◽  
Israel Figueiredo Jr ◽  
Salim Kanaan
Keyword(s):  
Creatine Kinase ◽  
Myocardial Ischemia ◽  
Premature Infants ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Isoenzyme Mb

Assay of creatine kinase isoenzyme MB in serum with time-resolved immunofluorometry

1990 ◽  
Vol 36 (9) ◽  
pp. 1679-1683 ◽  
Author(s):  
E S Lianidou ◽  
T K Christopoulos ◽  
E P Diamandis
Keyword(s):  
Creatine Kinase ◽  
Laser Excitation ◽  
Solid Phase ◽  
Time Resolved ◽  
Creatine Kinase Isoenzyme ◽  
Phase Complex ◽  
Precision And Accuracy ◽  
Accuracy Indices ◽  
First Time ◽  
Creatine Kinase Isoenzyme Mb

Abstract We describe the first time-resolved immunofluorometric assay for creatine kinase (EC 2.7.3.2) isoenzyme MB (CK-MB) in serum. The assay is based on the formation of the complex: solid-phase anti-CK-MB-CK-MB-biotinylated anti-CK-BB-streptavidin-BCPDA-Eu3+, where anti-CK-MB and anti-CK-BB are monoclonal antibodies against the CK isoenzymes MB and BB, respectively, and BCPDA is the europium chelator 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid. The solid-phase complex is fluorescent and is measured on the dry solid-phase (microtiter well) in a specially designed time-resolved fluorometer that uses laser excitation. The assay requires 25 microL of serum and is not affected by the presence of either CK-MM (up to 5000 micrograms/L) or CK-BB (up to 1000 micrograms/L) in the sample. Precision and accuracy indices for the assay were satisfactory.

Increased creatine kinase isoenzyme MB values in patients without myocardial infarct.

1978 ◽  
Vol 24 (10) ◽  
pp. 1818-1821 ◽  
Author(s):  
L M Shaw ◽  
D A Newman
Keyword(s):  
Myocardial Infarction ◽  
Intensive Care Unit ◽  
Creatine Kinase ◽  
Medical Intensive Care Unit ◽  
Myocardial Infarct ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Mb ◽  
Medical Intensive Care ◽  
Column Procedure ◽  
Creatine Kinase Isoenzyme Mb

Abstract Six of 13 randomly selected patients in a medical intensive-care unit with above-normal creatine kinase MB activities had diagnoses other than myocardial infarction. These data, which indicate the need for further study, were obtained during evaluation of a commercially available column procedure (Biodynamics/bmc).

Clinical evaluation of an immunoinhibition procedure for creatine kinase-MB.

1980 ◽  
Vol 26 (1) ◽  
pp. 150-152
Author(s):  
D Obzansky ◽  
J A Lott
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Creatine Kinase ◽  
Sensitivity And Specificity ◽  
Clinical Evaluation ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Mb ◽  
Diagnostic Sensitivity And Specificity ◽  
Creatine Kinase Isoenzyme Mb

Abstract We have clinically evaluated the Dade "Cardiozyme" immunoinhibition procedure for determination of creatine kinase isoenzyme MB (CK-MG) in 71 patients who were suspected of having had an acute myocardial infarction. Electrophoresis for CK-MB was also carried out. On the basis of diagnostic sensitivity and specificity for myocardial infarction, we found the Dade procedure for CK-MB to be somewhat inferior to electrophoresis. In 11 patients for whom the time of infarction was known, we observed normal CK-MB results for two of them by both immunoinhibition and electrophoresis during the first 24 h, but subsequently could detect abnormal CK-MB results by both methods. Thus in some patients such data are not helpful for making a diagnosis in the first 24 h. The Dade procedure is easy to perform, but lacks sensitivity in the region of low CK-MB activity, requires a very stable spectrophotometer, is imprecise, and produces negative numerical results in patients without myocardial infarction.

Diagnosis of acute myocardial infarction from two measurements of creatine kinase isoenzyme MB with use of nonparametric probability estimation.

1989 ◽  
Vol 35 (3) ◽  
pp. 444-447 ◽  
Author(s):  
L H Bernstein ◽  
I J Good ◽  
G I Holtzman ◽  
M L Deaton ◽  
J Babb
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Creatine Kinase ◽  
Bayes Factors ◽  
Probability Estimation ◽  
Posterior Odds ◽  
Creatine Kinase Isoenzyme ◽  
Time Of Admission ◽  
Bivariate Probability ◽  
Creatine Kinase Isoenzyme Mb

Abstract By using bivariate probability estimation for the diagnosis of acute myocardial infarction (AMI) we show how to overcome the difficulties encountered for patients whose clinical presentation is atypical and those encountered when multiple isoenzyme determinations are treated by univariate methods. We use the values for creatine kinase isoenzyme MB measured at the time of admission and 12 h later to estimate the Bayes factors in favor of AMI. The Bayes factors are compiled into a table that the clinician can use to estimate the posterior probability that a patient has AMI. The table of Bayes factors is based on data for a sample of 802 non-AMI patients and 180 AMI patients. Further to validate the method, we randomly chose 200 of the non-AMI and 50 of the AMI patients as an evaluation sample, then used the remaining 602 non-AMI and 130 AMI patients to recompute the Bayes factors. These Bayes factors were used to find the probability of AMI for each of the 250 patients in the evaluation sample. The method resulted in only one false positive and no false negatives. For the misclassified patient the measurements at admission and 12 h later were 1 and 11 U/L; the posterior odds were 15 to 1 in favor of AMI, but in fact the patient was non-AMI.

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