Carolina de Gouveia M. D. E. Pinheiro
◽
Fabiano Aurélio D. S. Oliveira
◽
Silvia Catarina S. Oloris
◽
Jean Berg A. da Silva
◽
Benito Soto-Blanco
AbstractThe present study aimed to detect pesticide residues in the honey produced by the stingless bee Melipona subnitida. A total of thirty-five samples of honey from M. subnitida were collected from twelve municipalities of the semiarid region of Rio Grande do Norte state, northeastern Brazil. Of these thirty-five samples, fourteen were from colonies raised in an urban area, while the other twenty-one were from the countryside. The pesticides in the samples were extracted using a modified QuEChERS method. The simultaneous analysis of 116 analytes in the honey samples was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Twenty-five samples (71.4% of samples) were contaminated by some amount of pesticide, and of them twenty-four had just one pesticide and one had three. The detected compounds included organophosphate pesticides (OPP) monocrotophos (24 samples), trichlorfon (6 samples) and chlorpyrifos-methyl (2 samples). In conclusion, the honey produced by the stingless bee M. subnitida may be contaminated by pesticides, which emphasizes the need for food monitoring before commercialization.
Isolation and characterization of 23 microsatellite loci in the stingless bee Melipona subnitida using next-generation sequencing
