Determination of Surface Complexation Constant of Methylene Blue,NH4+, Na+and K+with SiO2Surface

Poly(methylene blue) and electrochemically reduced graphene oxide composite film modified electrode (PMB-ERGO/GCE) was successfully fabricated by electropolymerization and was used for simultaneous determination of uric acid (UA) and xanthine (Xa). Based on the excellent electrocatalytic activity of PMB-ERGO/GCE, the electrochemical behaviors of UA and Xa were studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). Two anodic sensitive peaks at 0.630 V (versus Ag/AgCl) for UA and 1.006 V (versus Ag/AgCl) for Xa were given by CV in pH 3.0 phosphate buffer. The calibration curves for UA and Xa were obtained in the range of 8.00 × 10−8~4.00 × 10−4 M and 1.00 × 10−7~4.00 × 10−4 M, respectively, by SWV. The detection limits for UA and Xa were3.00×10-8 M and5.00×10-8 M, respectively. Finally, the proposed method was applied to simultaneously determine UA and Xa in human urine with good selectivity and high sensitivity.

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AMPEROMETRIC TITRATION OF PICROLONIC ACID AND INDIRECT VOLUMETRIC DETERMINATION OF CALCIUM BY PRECIPITATION AS PICROLONATE AND BACK TITRATION OF THE EXCESS OF PICROLONIC ACID WITH METHYLENE BLUE

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)72273-4 ◽

1943 ◽

Vol 148 (3) ◽

pp. 711-718

Author(s):

Gunther Cohn ◽

I.M. Kolthoff

Keyword(s):

Methylene Blue ◽

Amperometric Titration ◽

Back Titration ◽

Volumetric Determination ◽

Picrolonic Acid

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Determination of lethal concentration and antibacterial activity of commonly used disinfectants

International Journal of Natural Sciences ◽

10.3329/ijns.v1i4.9738 ◽

1970 ◽

Vol 1 (4) ◽

pp. 102-105 ◽

Cited By ~ 1

Author(s):

M Alam ◽

MM Rahman ◽

MJ Foysal ◽

MN Hossain

Keyword(s):

Methylene Blue ◽

Pseudomonas Fluorescens ◽

Potassium Permanganate ◽

Malachite Green ◽

Copper Sulfate ◽

Pathogenic Bacteria ◽

Labeo Rohita ◽

Inhibitory Effect ◽

Lethal Concentration

The toxic effects of four disinfectants viz., copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green on fish and fish pathogenic bacteria Aeromonas sp., Pseudomonas fluorescens, Edwardsiella sp. and Flavobacterium sp. were investigated. Lethal concentration of the disinfectants to fingerlings of Labeo rohita was determined in aquarium by standard method. Lethal concentration of copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green against fish were found in 0.75ppm, 7ppm, 6ppm and 0.5ppm at 21.4hrs, 18hrs, 9.5hrs and 1.40hrs, respectively. Methylene blue at 4ppm and 5ppm concentration inhibited the growth of Pseudomonas fluorescens and 6ppm concentration suppressed the growth of Aeromonas sp. Copper sulfate (CuSO4) was effective only against Edwardsiella sp at concentration of 10ppm and 8ppm. Malachite green repressed the growth of all four tasted bacteria at a concentration of 1ppm. Potassium permanganate (KMnO4) was failed to exhibit any inhibitory effect on the bacteria even at 30ppm concentration. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9738 IJNS 2011 1(4): 102-105

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