scholarly journals Determination of lethal concentration and antibacterial activity of commonly used disinfectants

1970 ◽  
Vol 1 (4) ◽  
pp. 102-105 ◽  
Author(s):  
M Alam ◽  
MM Rahman ◽  
MJ Foysal ◽  
MN Hossain
Keyword(s):  
Methylene Blue ◽  
Pseudomonas Fluorescens ◽  
Potassium Permanganate ◽  
Malachite Green ◽  
Copper Sulfate ◽  
Pathogenic Bacteria ◽  
Labeo Rohita ◽  
Inhibitory Effect ◽  
Lethal Concentration

The toxic effects of four disinfectants viz., copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green on fish and fish pathogenic bacteria Aeromonas sp., Pseudomonas fluorescens, Edwardsiella sp. and Flavobacterium sp. were investigated. Lethal concentration of the disinfectants to fingerlings of Labeo rohita was determined in aquarium by standard method. Lethal concentration of copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green against fish were found in 0.75ppm, 7ppm, 6ppm and 0.5ppm at 21.4hrs, 18hrs, 9.5hrs and 1.40hrs, respectively. Methylene blue at 4ppm and 5ppm concentration inhibited the growth of Pseudomonas fluorescens and 6ppm concentration suppressed the growth of Aeromonas sp. Copper sulfate (CuSO4) was effective only against Edwardsiella sp at concentration of 10ppm and 8ppm. Malachite green repressed the growth of all four tasted bacteria at a concentration of 1ppm. Potassium permanganate (KMnO4) was failed to exhibit any inhibitory effect on the bacteria even at 30ppm concentration. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9738 IJNS 2011 1(4): 102-105

Effects of Parasiticides on Nitrification

1975 ◽  
Vol 32 (11) ◽  
pp. 2033-2037 ◽  
Author(s):  
M. T. Collins ◽  
J. B. Gratzek ◽  
D. L. Dawe ◽  
T. G. Nemetz
Keyword(s):  
Methylene Blue ◽  
Single Dose ◽  
Sodium Chloride ◽  
Potassium Permanganate ◽  
Malachite Green ◽  
Copper Sulfate ◽  
Therapeutic Use ◽  
Rapid Rise ◽  
Complete Cessation ◽  
Nitrate Concentrations

Treatment of four recirculating systems with a single dose of methylene blue at 5 mg/liter resulted in complete cessation of nitrification for 16 days as evidenced by a rapid rise in ammonia and stable nitrate concentrations. The therapeutic use of methylene blue in recirculating systems is contraindicated. Treatment of the systems with therapeutic levels of formalin, malachite green, formalin and malachite green in combination, copper sulfate, potassium permanganate, and sodium chloride had no effect on nitrification.

scholarly journals DETERMINATION OF ANTIMICROBIALACTIVITY USING METHANOLIC EXTRACT OF PITHECELLOBIUM DULCE PLANT LEAVES.

2020 ◽  
pp. 1-3
Author(s):  
Mithaiwala Idris A. ◽  
Suthar Hetal S. ◽  
Hetvi Soni ◽  
Ojha Sunil ◽  
Agrawal Amit
Keyword(s):  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Plant Leaves ◽  
Bacterial Diseases ◽  
Gram Positive Bacteria ◽  
Soxhlet Method ◽  
Bacteria And Fungi ◽  
Antibacterial And Antifungal ◽  
Constitutional Analysis

Present days due to the pathogenic bacteria and fungi, the human mortality rate has increased in number. The synthetic antimicrobial and antifungal are used in the treatment of various fungal and bacterial diseases, but have various side effects which limit's their use. Traditionally Pithecellobium dulce plant has been used for treatment of antifungal and antimicrobial infection. In this article we are comparing the activity of antimicrobial and antifungal properties of Pithecellobium dulce plant using leaves extract on gram negative bacteria,gram positive bacteria and fungi.Methanolic extraction of Pithecellobium dulce leaves was carried out using Soxhlet method and the phyto constitutional analysis was carried out for presenceof alkaloids, avonoids, glycosides, saponins, phytosterols, and triterpenoids.The inhibitory effect was determined by Cup plate method, by the measurement of zone of inhibition.The outcome was the leaves extract consisted of active phytoconstituents which were phenols, glycoside, sugar and avanoid due to which the extract showed signicant zone of inhibition,by which property of antibacterial and antifungal was listed. It can be concluded that the leaves of Pithecellobium dulce consist of active phytoconstituents which can be used as potent antimicrobials

scholarly journals ICHTHYOPHTHIRIOSIS – CAUSE OF SIGNIFICANT LOSSES OF CARP FINGERLINGS

2016 ◽  
Vol 8 (1) ◽  
pp. 3-12
Author(s):  
Miroslav Ćirković ◽  
Nikolina Novakov ◽  
Brankica Kartalović ◽  
Miloš Pelić ◽  
Sanja Jovanić ◽  
...  
Keyword(s):  
Potassium Permanganate ◽  
Malachite Green ◽  
Copper Sulfate ◽  
Health Condition ◽  
Replication Rate ◽  
Green Is ◽  
Chemical Treatments ◽  
Prophylactic Measures ◽  
Parasite Replication ◽  
The Moment

Ichthyophthiriosis is widespread parasitic disease of fi shes caused by a ciliated protozoan, Ichthyophthirius multifi lis. Th is parasite is capable of affecting most of species of freshwater fi shes, including all cyprinids. The parasites usually can be found on skin and gill in the form of white prominent spots 0.1-1 mm in diameter, which looks as if the fi sh was sprinkled with grits. Within these tissues, infections cause localized lymphocyte infi ltration, focal necrosis and varying degrees of epithelial proliferation. Investigations were carrieed out during a last 10 years, while monitoring the health condition of carp fi sh. Diagnosis of ichthyophthiriosis was performed by clinical and microscopic examination. Disease was present in carps throughout their life, but the most susceptible were young categories. The parasites were present on the skin and gills earlier in 10-day-old fi ngerlings. The outbreaks are most common in spring, aft er overwintering when water temperatures increase and also does the parasite replication rate. High stock density, water quality and poor condition contribute to illness. Since the disease causes signifi cant losses of carp fingerlings it is necessary to perform its adequate controls and therapy. In order to prevent the disease it is need to to rear young fi sh separately from other fish categories, prevent weed fi shes from entering the ponds and employ hygienic and prophylactic measures. All technological measures which can improve the condition of fi sh are most eff ective against ichthyophthiriosis. It is important to add lime into the pond from time to time. Eff ective chemical treatments for I.multifi liis include copper sulfate, potassium permanganate, malachite green, salt and formalin. Malachite green is a teratogen, and prohibited for use, while copper sulfate, potassium permanganate and formalin are also under currently reviewing for the use as parasiticides in food fi sh. So that, non-iodized salt is the only permitted and safe therapeutic for the moment.

Determination of malachite green, crystal violet, brilliant green and methylene blue by micro-cloud-point extraction and nonlinear laser wave-mixing detection interfaced to micellar capillary electrophoresis

2017 ◽  
Vol 9 (45) ◽  
pp. 6411-6419 ◽  
Author(s):  
Jean Sebastien Pradel ◽  
William G. Tong
Keyword(s):  
Methylene Blue ◽  
Capillary Electrophoresis ◽  
Malachite Green ◽  
Crystal Violet ◽  
Brilliant Green ◽  
Cloud Point Extraction ◽  
Detection Method ◽  
Wave Mixing ◽  
Laser Wave

A laser wave-mixing CE detection method is reported for separation and detection of malachite green (MG), crystal violet (CV), brilliant green (BG), methylene blue (MB), and the leuco-metabolites of MG and CV residues in aquacultures.

scholarly journals Kinetics Spectrophotometric determination of Ranitidine based on its Inhibitory effect on the Oxidation Rate of Malachite green by N-bromosuccinide

2020 ◽  
Vol 13 (1) ◽  
pp. 253-261
Author(s):  
Ashraf M. Taha ◽  
Alaa Eldin Mokhtar Abdel-Hady
Keyword(s):  
Malachite Green ◽  
Inhibitory Effect ◽  
Calibration Graph ◽  
Rate Of Change ◽  
Linear Calibration ◽  
Inhibiting Effect ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A simple and sensitive kinetic spectrophotometric method is described for the determination of ranitidine. The method is based on the inhibiting effect of ranitidine on the rate of oxidation of malachite green (MG+ ) with N-bromosuccinimide (NBS) The oxidation reaction was followed spectrophotometrically by measuring the rate of change of the absorbance of malachite green with time at λ=617nm in the presence of different concentrations of ranitidine using the recommended procedure. Ranitidine can be determined from 0.08 to 2.40 g ml-1 with a linear calibration graph and detection limit of 0.026 g ml-1 . The method was successfully applied for the determination of ranitidine in pure ranitidine samples and in ranitidine tablets. The recovery of the analyzed samples were 97-100% with relative standard deviation, sr (%) =1.14 x 10-4 indicating high accuracy and precision of the suggested method. The interference of various cations and anions in the determination of ranitidine was studied

Sign in / Sign up

Export Citation Format

Share Document