scholarly journals El ADN reescribe nuestra memoria

Author(s):  
Mercedes Aler Gay ◽  
Ángel Carracedo Álvarez

Continuous advances in DNA analysis for forensic purposes have set milestones in the search for genetic identity in criminal cases, disasters, and disappearances. Technological advances in the study of our genome allow us to infer who was the owner of remains found, for example, in a mass grave or an anonymous tomb: where they lived, their physical appearance, or their family origin. The analysis of DNA of forensic interest manages, thanks to a series of fixed variations between individuals, to identify them individually via their genetic profile. This identification can be carried out by comparing the profile of the human remains with known profiles or by their compatibility with the DNA inherited by their relatives.

2020 ◽  
pp. 15-33
Author(s):  
Henry Erlich

Chapter 1 reviews the history of DNA analysis for individual identification in criminal cases. The principles underlying Restriction Fragment Length Polymorphism (RFLP) and Polymerase Chain Reaction (PCR) and their application in the first cases in the US and the UK in the mid-‘80s are discussed. The differences between these two DNA technologies (RFLP and PCR) are discussed and the evolution of new PCR-based genotyping methods for analyzing length and sequence polymorphisms is reviewed. The first DNA exoneration, which used the PCR-based HLA-DQ alpha test, is discussed in the context of exclusionary and inclusionary DNA results. The statistical issues involved in interpreting a match (inclusion) between the genetic profile of the evidence and the reference samples by calculating the Random Match Probability metric is discussed. Finally, the contentious history of the debate about the admissibility of DNA results in the courtroom, known as the “DNA Wars” is reviewed.


2020 ◽  
Vol 17 (34) ◽  
pp. 93-111
Author(s):  
Saule Amangeldievna MUSSABEKOVA

Identifying semen stains on clothing and bedding is a crucial component in investigating cases of sexual assault. In some cases, clothing and bedding have already been washed before they were removed and sent for forensic examination. There is insufficient data on best practices for handling traces of semen on clothes after washing. This work aimed to study the possibility of identifying traces of semen on clothes after washing using widely used techniques. To simulate typical physical evidence, donor semen samples were applied to pieces of clothing made from various fabrics. The clothes were washed under multiple conditions (with the help of nonbiological and biological (enzyme-containing) agents, and across numerous washing machines). After washing, the washing stains were characterized by the presence of fluorescence signal, spermatozoa (Koren – Stokis method), the determination of acid phosphatase, prostate-specific antigen and semenogelin, as well as the results of serological research according to the AB0 system and DNA analysis. Clothing analysis using these methods was shown to be effective in experimental conditions. However, the presence of enzymes as detergent components designed to destroy biogenic stains significantly affect the results of the identification of semen stains. It has been established that the full genetic profile can be obtained from semen stains even after washing three times. Different strategies are needed to detect, select and identify semen stains depending on the circumstances of a case. It is recommended to examine clothing and bedding, even if the specimens were previously washed.


2020 ◽  
pp. 363-372

The first applications of DNA technology in criminal cases took place in the United States and United Kingdom more than 30 years ago. What have we learned over the past three decades from the use of forensic DNA analysis in criminal and human rights investigations and humanitarian disasters? And what challenges, opportunities, and potential pitfalls lie ahead?...


2018 ◽  
Vol 17 ◽  
pp. 943-949 ◽  
Author(s):  
Esther J. Lee ◽  
D. Andrew Merriwether ◽  
Alexei K. Kasparov ◽  
Valery I. Khartanovich ◽  
Pavel A. Nikolskiy ◽  
...  

Author(s):  
Nor Aidora Saedon ◽  
Mohd Zuwairi Saiman ◽  
Noraini Ahmad ◽  
Rauzah Hashim ◽  
Sharifah Nur Hafiza Syed A.Rahman ◽  
...  
Keyword(s):  

2014 ◽  
Vol 19 (1) ◽  
pp. 1-14
Author(s):  
Jan Cemper-Kiesslich ◽  
Mark R. McCoy ◽  
Fabian Kanz

Genetic information discovered, characterized for and used in forensic case-works and anthropology has shown to be also highly useful and relevant in investigating human remains from archaeological findings. By technical means, forensic and aDNA (ancient Deoxyribonucleic acid) analyses are well suited to be done using the same laboratory infrastructures and scientific expertise referring to sampling, sample protection, sample processing, contamination control as well as requiring analogous technical know how and knowledge on reading and interpreting DNA encoded information. Forensic genetics has significantly profited from aDNA-related developments (and vice versa, of course!), especially, when it comes to the identification of unknown human remains referring to the detection limit. Additionally the tremendous developments of analyzing chemistry and kits as well as instruments in forensics opened the whole panel of reading human and nonhuman DNA for historians and archaeologists but also for anthropologists. Ancient DNA / molecular archaeology, however, is not limited to the comparatively restrictive set of information as usually employed in forensic case work analyses but can also be applied to phenotypical markers, ethno-related genotypes or pathological features.In this review the authors give a general overview on the field of ancient DNA analysis focussing of the potentials and limits, fields of application, requirements for samples, laboratory setup, reaction design and equipment as well as a brief outlook on current developments, future perspectives and potential cross links with associated scientific disciplines.Key words: Human DNA, Ancient DNA, Forensic DNA typing, Molecular archaeology, Application.


2021 ◽  
Vol 16 (1) ◽  
pp. 6-18
Author(s):  
S. A. Smirnova ◽  
G. G. Omel’yanyuk ◽  
I. V. Storozhenko ◽  
A. A. Rybakova ◽  
V. V. Gulevskaya

The article addresses the importance and basic preconditions for the forming a new direction of forensic activity in the system of forensic institutions of the Russian Ministry of Justice a new direction of forensic activity - molecular-genetic analysis of the objects of biological origin. The authors present the advantages of DNA analysis - one of the most modern and efficient methods in investigating criminal cases. The article also demonstrates the potential of different methods of DNA-analysis for forensic investigations. The history of forensic DNA-analysis development in Russia and its features when examining the human, animal, and plant biomaterials are briefly discussed. The authors propose the definitions for the molecular-genetic examinations’ object and subject, formulate the model tasks, and suggest a list of sample questions for this study.


Britannia ◽  
2019 ◽  
Vol 50 ◽  
pp. 203-224 ◽  
Author(s):  
Trudi Buck ◽  
Elizabeth M. Greene ◽  
Alexander Meyer ◽  
Victoria Barlow ◽  
Eleanor Graham

ABSTRACTDisarticulated human remains were recovered from a first-century fort ditch at Vindolanda on the north-west frontier of the Roman Empire. Ancient DNA analysis revealed the skeleton to be that of a male individual and forensic taphonomic analysis suggested a primary deposition of the body in a waterlogged environment with no obvious evidence of formal burial. Occurrences of disarticulated human remains outside a cemetery context are often overlooked in Roman bioarchaeology. This discovery adds to the growing body of literature regarding alternative funerary practice in the Empire, highlighting that the concept of burial and disposal of the dead is more complex than ancient historical sources suggest. Details of the DNA analysis are provided in the Supplementary Material available at https://doi.org/10.1017/S0068113X1900014X.


Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 433 ◽  
Author(s):  
Maxime Merheb ◽  
Rachel Matar ◽  
Rawad Hodeify ◽  
Shoib Sarwar Siddiqui ◽  
Cijo George Vazhappilly ◽  
...  

Mitochondria are unique organelles carrying their own genetic material, independent from that in the nucleus. This review will discuss the nature of mitochondrial DNA (mtDNA) and its levels in the cell, which are the key elements to consider when trying to achieve molecular identification in ancient and degraded samples. mtDNA sequence analysis has been appropriately validated and is a consistent molecular target for the examination of biological evidence encountered in forensic cases—and profiling, in certain conditions—especially for burnt bodies and degraded samples of all types. Exceptional cases and samples will be discussed in this review, such as mtDNA from leather in Beethoven’s grand piano, mtDNA in mummies, and solving famous historical criminal cases. In addition, this review will be discussing the use of ancient mtDNA to understand past human diet, to trace historical civilizations and ancient trade routes, and to uncover geographical domestication origins and lineage relationships. In each topic, we will present the power of mtDNA and how, in many cases, no nuclear DNA was left, leaving mitochondrial DNA analysis as a powerful alternative. Exploring this powerful tool further will be extremely useful to modern science and researchers, due to its capabilities in providing us with previously unattainable knowledge.


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