scholarly journals Peer Review #3 of "Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress (v0.1)"

2019 ◽  
Keyword(s):  
Thermal Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Peer Review ◽  
Bemisia Tabaci ◽  
Small Heat Shock Proteins ◽  
Genes Encoding ◽  
Shock Proteins

scholarly journals Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6992 ◽  
Author(s):  
Jing Bai ◽  
Xiao-Na Liu ◽  
Ming-Xing Lu ◽  
Yu-Zhou Du
Keyword(s):  
Thermal Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Temperature Stress ◽  
Bemisia Tabaci ◽  
Stress Proteins ◽  
Plant Viruses ◽  
Small Heat Shock Proteins ◽  
Amino Acid Sequences ◽  
Shock Proteins

Small heat shock proteins (sHSPs) are probably the most diverse in structure and function among the various super-families of stress proteins, and they play essential roles in various biological processes. The sweet potato whitefly, Bemisia tabaci (Gennadius), feeds in the phloem, transmits several plant viruses, and is an important pest on cotton, vegetables and ornamentals. In this research, we isolated and characterized three α-crystallin/sHSP family genes (Bthsp19.5, Bthsp19.2, and Bthsp21.3) from Bemisia tabaci. The three cDNAs encoded proteins of 171, 169, and 189 amino acids with calculated molecular weights of 19.5, 19.2, and 21.3 kDa and isoelectric points of 6.1, 6.2, and 6.0, respectively. The deduced amino acid sequences of the three genes showed strong similarity to sHSPs identified in Hemiptera and Thysanoptera insects species. All three sHSPs genes from Bemisia tabaci lacked introns. Quantitative real-time PCR analyses revealed that the three BtsHSPs genes were significantly up-regulated in Bemisia tabaci adults and pupae during high temperature stress (39, 41, 43, and 45 °C) but not in response to cold temperature stress (−6, −8, −10, and −12 °C). The expression levels of Bthsp19.2 and Bthsp21.3 in pupae was higher than adults in response to heat stress, while the expression level of Bthsp19.5 in adults was higher than pupae. In conclusion, this research results show that the sHSP genes of Bemisia tabaci had shown differential expression changes under thermal stress.

scholarly journals Characterization of rice small heat shock proteins targeted to different cellular organelles

2015 ◽  
Vol 20 (3) ◽  
pp. 451-460 ◽  
Author(s):  
Nandini Mani ◽  
Krishnaveni Ramakrishna ◽  
Kaza Suguna
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Small Heat Shock Proteins ◽  
Shock Proteins ◽  
Cellular Organelles
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