scholarly journals Peer Review #3 of "Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function (v0.1)"

2020 ◽  
Keyword(s):  
Brassica Napus ◽  
Peer Review ◽  
Cuticular Wax ◽  
Wax Deposition ◽  
Exogenous Hormones

scholarly journals Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9264 ◽  
Author(s):  
Zheng Yuan ◽  
Youwei Jiang ◽  
Yuhua Liu ◽  
Yi Xu ◽  
Shuai Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Water Loss ◽  
Loss Rate ◽  
Secondary Alcohol ◽  
Cuticular Wax ◽  
Chemical Compositions ◽  
Wax Deposition ◽  
Water Loss Rate ◽  
Leaf Wax ◽  
Plant Adaptations

Background Cuticular waxes cover plant surface and play important roles in protecting plants from abiotic and biotic stresses. The variations of wax deposition and chemical compositions under changing environments have been shown to be related to plant adaptations. However, it is still not clear whether the wax depositions could be adjusted to increase plant adaptations to stressed conditions. Methods In this study, exogenous methyl jasmonate (MeJA), the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and salicylic acid (SA) were applied to test their effects on cuticular wax deposition in two Brassica napus cultivars, Zhongshuang 9 (ZS9, low wax coverage ) and Yuyou 19 (YY19, high wax coverage). Next, we measured the water loss rate and the transcriptional expression of genes involved in wax biosynthesis as well as genes related to disease defense. Results Seven wax compound classes, including fatty acids, aldehydes, alkanes, secondary alcohols, ketones, and unbranched as well as branched primary alcohols, were identified in B. napus leaf wax mixtures. MeJA, SA and ACC treatments had no significant effect on total wax amounts in YY19, whereas ACC reduced total wax amounts in ZS9. Overall, hormone treatments led to an increase in the amounts of aldehydes and ketones, and a decrease of secondary alcohol in ZS9, whereas they led to a decrease of alkane amounts and an increase of secondary alcohol amounts in YY19. Concomitantly, both cultivars also exhibited different changes in cuticle permeability, with leaf water loss rate per 15 min increased from 1.57% (averaged across treatments) at 1.57% (averaged across treatments) at 15 min to 3.12% at 30 min for ZS9 (except for ACC treated plant) and decreased for YY19. MeJA-treated plants of both cultivars relatively had higher water loss rate per 15 min when compared to other treatments. Conclusion. Our findings that B. napus leaf wax composition and cuticle permeability are altered by exogenous SA, MeJA and ACC suggest that the hormone treatments affect wax composition, and that the changes in wax profiles would cause changes in cuticle permeability.

Effects of SA, MeJA, and ACC on Leaf Cuticular Wax Constituents, Structure and Permeability in Brassica napus

2016 ◽  
Vol 42 (12) ◽  
pp. 1827
Author(s):  
Shuai LI ◽  
Qiu-Ling ZHAO ◽  
Yang PENG ◽  
Yi XU ◽  
Jia-Na LI ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Cuticular Wax

scholarly journals Dissecting the Roles of Cuticular Wax in Plant Resistance to Shoot Dehydration and Low-Temperature Stress in Arabidopsis

2021 ◽  
Vol 22 (4) ◽  
pp. 1554
Author(s):  
Tawhidur Rahman ◽  
Mingxuan Shao ◽  
Shankar Pahari ◽  
Prakash Venglat ◽  
Raju Soolanayakanahally ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Low Temperature ◽  
Cold Acclimation ◽  
Water Loss ◽  
Cuticular Wax ◽  
Dehydration Stress ◽  
Wax Deposition ◽  
Cuticular Waxes ◽  
Gas Chromatography Mass Spectroscopy

Cuticular waxes are a mixture of hydrophobic very-long-chain fatty acids and their derivatives accumulated in the plant cuticle. Most studies define the role of cuticular wax largely based on reducing nonstomatal water loss. The present study investigated the role of cuticular wax in reducing both low-temperature and dehydration stress in plants using Arabidopsis thaliana mutants and transgenic genotypes altered in the formation of cuticular wax. cer3-6, a known Arabidopsis wax-deficient mutant (with distinct reduction in aldehydes, n-alkanes, secondary n-alcohols, and ketones compared to wild type (WT)), was most sensitive to water loss, while dewax, a known wax overproducer (greater alkanes and ketones compared to WT), was more resistant to dehydration compared to WT. Furthermore, cold-acclimated cer3-6 froze at warmer temperatures, while cold-acclimated dewax displayed freezing exotherms at colder temperatures compared to WT. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis identified a characteristic decrease in the accumulation of certain waxes (e.g., alkanes, alcohols) in Arabidopsis cuticles under cold acclimation, which was additionally reduced in cer3-6. Conversely, the dewax mutant showed a greater ability to accumulate waxes under cold acclimation. Fourier Transform Infrared Spectroscopy (FTIR) also supported observations in cuticular wax deposition under cold acclimation. Our data indicate cuticular alkane waxes along with alcohols and fatty acids can facilitate avoidance of both ice formation and leaf water loss under dehydration stress and are promising genetic targets of interest.

scholarly journals Overexpression of BnKCS1-1, BnKCS1-2, and BnCER1-2 promotes cuticular wax production and increases drought tolerance in Brassica napus

2020 ◽  
Vol 8 (1) ◽  
pp. 26-37 ◽  
Author(s):  
Yanmei Wang ◽  
Shurong Jin ◽  
Yi Xu ◽  
Shuai Li ◽  
Shuangjuan Zhang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Drought Tolerance ◽  
Cuticular Wax ◽  
Wax Production

Cuticular wax deposition in growing barley (Hordeum vulgare) leaves commences in relation to the point of emergence of epidermal cells from the sheaths of older leaves

Planta ◽  
2005 ◽  
Vol 222 (3) ◽  
pp. 472-483 ◽  
Author(s):  
Andrew Richardson ◽  
Rochus Franke ◽  
Gerhard Kerstiens ◽  
Mike Jarvis ◽  
Lukas Schreiber ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Epidermal Cells ◽  
Cuticular Wax ◽  
Wax Deposition
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