scholarly journals Establishment of in vitro regeneration from petiole explants and assessment of clonal fidelity by ISSR markers in Luffa acutangula L.Roxb

2016 ◽  
Keyword(s):  
In Vitro Regeneration ◽  
Issr Markers ◽  
Clonal Fidelity ◽  
Petiole Explants ◽  
Luffa Acutangula

scholarly journals Silver nanoparticles induce genetic, biochemical, and phenotype variation in chrysanthemum

2020 ◽  
Vol 143 (2) ◽  
pp. 331-344
Author(s):  
Alicja Tymoszuk ◽  
Dariusz Kulus
Keyword(s):  
Silver Nanoparticles ◽  
In Vitro Regeneration ◽  
Adventitious Shoots ◽  
Issr Markers ◽  
Phenotype Variation ◽  
Inter Simple Sequence Repeats ◽  
Tremendous Progress ◽  
Genetic Level ◽  
User Friendly

Abstract Despite the tremendous progress in breeding, novel and user-friendly techniques of plant improvement are desirable. The study aimed to analyze the usefulness of silver nanoparticles (AgNPs) in the breeding of chrysanthemum: one of the top ornamental plant species. In vitro regeneration of adventitious shoots from internodes of chrysanthemum ‘Lilac Wonder’ was induced on the modified Murashige and Skoog (MS) medium supplemented with 0.6 mg L−1 6-benzylaminopurine (BAP), 2 mg L−1 indole-3-acetic acid (IAA) and AgNPs at 0, 5, 10 and 20 ppm concentration. The efficiency of callogenesis and caulogenesis were analyzed after 10 weeks of culture. The concentration of chlorophylls, carotenoids, and phenolic compounds in shoots and calli were estimated. Plants obtained from 20 ppm AgNPs treatment were additionally analyzed on the genetic level using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. In vitro rooted shoots were acclimatized in the glasshouse and subjected to biochemical and phenotype stability evaluation. AgNPs at the highest concentration (20 ppm) suppressed both callogenesis and caulogenesis in vitro. The concentration of metabolites in callus was stable, regardless of AgNPs treatment, except for carotenoids which production was enhanced by 20 ppm AgNPs. In contrast, the content of chlorophyll a and b in shoots varied depending on AgNPs treatment. Polymorphic loci were detected in 12 and 9 AgNPs-treated-plants by RAPD and ISSR markers, respectively (one of which was common to both marker systems). Rooting and acclimatization were fully successful in all experimental combinations. Phenotype alternations were detected in six plants; one from 10 ppm AgNPs treatment and five from 20 ppm treatment. They included variation in pigment content (anthocyanins and carotenoids) and/or inflorescence shape. Interestingly, only two plants revealed both genetic and phenotype polymorphisms. No genetic or phenotype variation was detected in the control plants. In conclusion, AgNPs can be used in chrysanthemum breeding.

In vitro regeneration from petiole explants of non-toxic Jatropha curcas

2011 ◽  
Vol 33 (1) ◽  
pp. 146-151 ◽  
Author(s):  
Nitish Kumar ◽  
K.G. Vijay Anand ◽  
Muppala P. Reddy
Keyword(s):  
Jatropha Curcas ◽  
In Vitro Regeneration ◽  
Petiole Explants

scholarly journals In vitro regeneration and PCR-RAPD based detection of somaclonal variation in kenaf (Hibiscus cannabinus)

2017 ◽  
Vol 28 (2) ◽  
pp. 100-108
Author(s):  
MS Haque ◽  
T Biswas ◽  
MS Islam ◽  
MS Hossain
Keyword(s):  
Somaclonal Variation ◽  
Rapd Markers ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Hibiscus Cannabinus ◽  
Petiole Explants ◽  
Napthaleneacetic Acid ◽  
Mother Plants ◽  
Regenerated Plantlets

Though direct systems of regeneration through culture of organized meristems usually produce true-to-type plants, variations in the progenies have widely been reported. Fiber producing kenaf plants (Hibiscus cannabinus L.) were regenerated from petiole, hypocotyls and cotyledonous petiole explants on MS medium containing BAP (benzyl amino purine) and NAA (?-napthaleneacetic acid) followed by assessment of regenerants by RAPD markers to detect somaclonal variation among them. Genomic DNA from twenty seven plants [three mother plants and two clones (clone 1 and 2) from each mother plant with three replications] was subjected to random amplified polymorphic DNA (RAPD) analysis. Fifteen polymorphic loci amplified by three decamer random primers were used to estimate genetic diversity and relatedness in mother plants and their regenerated plantlets. The results showed some degree of polymorphism between mother plants and their regenerated plantlets as well as between regenerated plantlets indicating somaclonal variation among the regenerants. These suggest that the RAPD technique could effectively be used to detect somaclonal variation in H. cannabinus and could be promising for the detection of markers associated with desirable traits.Progressive Agriculture 28 (2): 100-108, 2017

scholarly journals High Frequency In vitro Regeneration of Gynura procumbens (Lour.) Merr.

2017 ◽  
Vol 27 (2) ◽  
pp. 207-216
Author(s):  
Tanjina Akhtar Banu ◽  
Barna Goswami ◽  
Shahina Akter ◽  
Mousona Islam ◽  
Tammana Tanjin ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Root Induction ◽  
Petiole Explants ◽  
Number Of Shoots

An efficient rapid in vitro regeneration protocol was described from nodal segment, leaf and petiole explants. MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IAA was found best for the multiple shoot formation from nodal segments. In this combination 99% explants produced multiple shoots and the average number of shoots per explants was 20.1 ± 1.96. For petiole and leaf explants best response was observed on MS supplemented with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. Petiole explants produced highest mean number of shoots/explant (22.9 ± 1.728) among the three explants when the explants were cultured on MS with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. The highest frequency of root induction (100%) and mean number of roots/plantlets (11.75) were obtained on MS. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Plant Tissue Cult. & Biotech. 27(2): 207-216, 2017 (December)

scholarly journals AN EFFICIENT IN VITRO REGENERATION METHOD TO PRODUCE ADVENTITIOUS PLANTS IN SWEETPOTATO

HortScience ◽  
1993 ◽  
Vol 28 (4) ◽  
pp. 262B-262
Author(s):  
C. S. Prakash ◽  
R. Gosukonda ◽  
A. Porobo Dessai ◽  
E. Blay ◽  
K. Dumenvo
Keyword(s):  
Gene Transfer ◽  
High Frequency ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Zeatin Riboside ◽  
Limiting Factor ◽  
Ms Medium ◽  
Petiole Explants ◽  
Green House

Lack of suitable methods to develop adventitious plantlets in vitro is a limiting factor in producing transgenic sweetpotato plants through gene transfer. Studies were conducted to develop an in vitro high frequency regeneration protocol for sweetpotato that is rapid and consistent. When 27 genotypes of sweetpotato were screened, five were identified as highly regenerative (318846-3, PI 531143, Hi Dry, Rojoblanco and Beauregard). High frequency regeneration of shoots (in 60 to 80% explants) was observed within 30 days when leaf explants with intact petioles from the apical portions of the in vitro shoots were cultured on a MS medium with 2,4-D (0.2 mg/l) for three days and then transferred to a medium with zeatin riboside (ZR) (0.2 mg/l). However, thidiazuron (0.2 mg/l) had to be substituted for ZR to achieve regeneration of shoots from petiole (0.5 to 1 cm) explants (the most responsive organ for transformation by Agrobacterium). Petiole explants developed shoots efficiently (80-90%) and rapidly (10 to 21 d), but were specific to the genotype 318846-3. The resulting plantlets were vigorous and normal, and were transferred to the green house with little or no mortality.

In vitro regeneration and assessment of genetic fidelity of acclimated plantlets by using ISSR markers in PPR-1 (Morus sp.): An economically important plant

2018 ◽  
Vol 241 ◽  
pp. 313-321 ◽  
Author(s):  
Gulab Khan Rohela ◽  
Phanikanth Jogam ◽  
Aftab Ahmad Shabnam ◽  
Pawan Shukla ◽  
Sadanandam Abbagani ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Genetic Fidelity ◽  
Issr Markers
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