scholarly journals Identification of dengue virus proteome B-cell epitopes using an immunoinformatic approach

2021 ◽  
Author(s):  
Neeraj Kumar Dixit ◽  
Anup Kumar
Keyword(s):  
Dengue Virus ◽  
B Cell ◽  
B Cell Epitopes

scholarly journals Comparison of Neutralizing Dengue Virus B Cell Epitopes and Protective T Cell Epitopes With Those in Three Main Dengue Virus Vaccines

2021 ◽  
Vol 12 ◽  
Author(s):  
Josilene Ramos Pinheiro ◽  
Esther Camilo dos Reis ◽  
Rayane da Silva Oliveira Souza ◽  
Ana Luíza Silva Rocha ◽  
Lincoln Suesdek ◽  
...  
Keyword(s):  
T Cell ◽  
Dengue Virus ◽  
B Cell ◽  
Public Health Problem ◽  
Phase Iii ◽  
World Health ◽  
Human Populations ◽  
T Cell Epitopes ◽  
Dengue Vaccine ◽  
B Cell Epitopes

The four serotypes of Dengue virus (DENV1-4) are arboviruses (arthropod-borne viruses) that belong to the Flavivirus genus, Flaviviridae family. They are the causative agents of an infectious disease called dengue, an important global public health problem with significant social-economic impact. Thus, the development of safe and effective dengue vaccines is a priority according to the World Health Organization. Only one anti-dengue vaccine has already been licensed in endemic countries and two formulations are under phase III clinical trials. In this study, we aimed to compare the main anti-dengue virus vaccines, DENGVAXIA®, LAV-TDV, and TAK-003, regarding their antigens and potential to protect. We studied the conservation of both, B and T cell epitopes involved in immunological control of DENV infection along with vaccine viruses and viral isolates. In addition, we assessed the population coverage of epitope sets contained in each vaccine formulation with regard to different human populations. As main results, we found that all three vaccines contain the main B cell epitopes involved in viral neutralization. Similarly, LAV-TDV and TAK-003 contain most of T cell epitopes involved in immunological protection, a finding not observed in DENGVAXIA®, which explains main limitations of the only licensed dengue vaccine. In summary, the levels of presence and absence of epitopes that are target for protective immune response in the three main anti-dengue virus vaccines are shown in this study. Our results suggest that investing in vaccines that contain the majority of epitopes involved in protective immunity (cellular and humoral arms) is an important issue to be considered.

scholarly journals Comprehensive mapping of immunodominant and conserved serotype- and group-specific B-cell epitopes of nonstructural protein 1 from dengue virus type 1

Virology ◽  
2010 ◽  
Vol 398 (2) ◽  
pp. 290-298 ◽  
Author(s):  
Yue Chen ◽  
Yuxian Pan ◽  
Yonghui Guo ◽  
Liwen Qiu ◽  
Xixia Ding ◽  
...  
Keyword(s):  
Dengue Virus ◽  
B Cell ◽  
Virus Type ◽  
Nonstructural Protein ◽  
B Cell Epitopes ◽  
Nonstructural Protein 1 ◽  
Comprehensive Mapping

scholarly journals Computational antigenic epitope prediction of clinical Indonesian Dengue virus NS1 protein

2021 ◽  
Vol 948 (1) ◽  
pp. 012080
Author(s):  
S Pambudi ◽  
D Irawan ◽  
A Danny ◽  
T Widayanti ◽  
Tarwadi
Keyword(s):  
T Cell ◽  
Dengue Virus ◽  
B Cell ◽  
Dengue Infection ◽  
Epitope Prediction ◽  
Antigenic Epitope ◽  
Ns1 Protein ◽  
Mhc I ◽  
B Cell Epitopes ◽  
Denv Serotypes

Abstract The identification of human Non-Structural-1 (NS1) protein epitopes will help us better understand Dengue virus (DENV) immunopathogenesis. In this study, several online and offline bioinformatic prediction tools were exploited to predict and analyze T-cell and B-cell epitopes of DENV NS1 consensus sequences originated from Indonesian clinical isolates. We identified a potential peptide at NS1155--163 (VEDYGFGIF) which interact with MHC-I allele HLA-B*40:01 and showed high binding affinity (IC50) scores ranging between 63.8 nM to 183.9 nM for all Indonesian DENV serotypes. Furthermore, we have succeeded identified a region at the C-terminal of Indonesian DENV NS1 protein between 325--344 as part of discontinuous antigenic epitope which conserved for all serotypes. Our analyses showed this region could induce strong and persistent antibody against all DENV serotypes by interacting with MHC-I molecule and also recognized by B-cell receptor. The identification of DENV NS1 T-cell and B-cell epitopes may help in the development of a new vaccine, drug discovery, and diagnostic system to help eradicate dengue infection.

scholarly journals Characterisation of B cell epitopes of dengue virus NS1 protein using bioinformatics approach

2016 ◽  
Vol 44 (4) ◽  
pp. 417 ◽  
Author(s):  
PD Pushpakumara ◽  
PH Premarathne ◽  
CL Goonasekara
Keyword(s):  
Dengue Virus ◽  
B Cell ◽  
Ns1 Protein ◽  
B Cell Epitopes

scholarly journals Correction: Identification of Continuous Human B-Cell Epitopes in the Envelope Glycoprotein of Dengue Virus Type 3 (DENV-3)

PLoS ONE ◽  
2009 ◽  
Vol 4 (10) ◽  
Author(s):  
Andréa N. M. Rangel da Silva ◽  
Eduardo J. M. Nascimento ◽  
Marli Tenório Cordeiro ◽  
Laura H. V. G. Gil ◽  
Frederico G. C. Abath ◽  
...  
Keyword(s):  
Dengue Virus ◽  
B Cell ◽  
Virus Type ◽  
Envelope Glycoprotein ◽  
B Cell Epitopes ◽  
Dengue Virus Type 3 ◽  
Type 3 ◽  
Human B Cell

scholarly journals Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients

2019 ◽  
Vol 8 (7) ◽  
Author(s):  
Siti Naqiah Amrun ◽  
Wearn‐Xin Yee ◽  
Farhana Abu Bakar ◽  
Bernett Lee ◽  
Yiu‐Wing Kam ◽  
...  
Keyword(s):  
Dengue Virus ◽  
B Cell ◽  
Virus Infections ◽  
B Cell Epitopes ◽  
Linear B

scholarly journals Identification and characterization of a linearized B-cell epitope on the pr protein of dengue virus

2013 ◽  
Vol 94 (7) ◽  
pp. 1510-1516 ◽  
Author(s):  
Ke-Yu Song ◽  
Hui Zhao ◽  
Shi-Hua Li ◽  
Xiao-Feng Li ◽  
Yong-Qiang Deng ◽  
...  
Keyword(s):  
Dengue Virus ◽  
B Cell ◽  
Cell Epitope ◽  
Denv Infection ◽  
Site Directed Mutagenesis ◽  
B Cell Epitopes ◽  
Virion Assembly ◽  
Pr Protein ◽  
B Cell Epitope ◽  
Hyperimmune Antiserum

The four serotypes of dengue virus (DENV) represent one of the major mosquito-borne pathogens globally; so far no vaccine or specific antiviral is available. During virion maturation, the pr protein is cleaved from its precursor form the prM protein on the surface of immature DENV by host protease. Recent findings have demonstrated that the pr protein not only played critical roles in virion assembly and maturation, but was also involved in antibody-dependent enhancement of DENV infection. However, the B-cell epitopes on the pr protein of DENV have not been well characterized. In this study, a set of 11 partially overlapping peptides spanning the entire pr protein of DENV-2 were fused with glutathione S-transferase and expressed in Escherichia coli. ELISA screening with murine hyperimmune antiserum against immature DENV identified the P8 peptide (57KQNEPEDIDCWCNST71) in the pr protein as the major immunodominant epitope. Fine mapping by truncated protein assays confirmed the 8-e peptide 57KQNEPEDI64 was the smallest unit capable of antibody binding. Importantly, the 8-e epitope reacted with sera from dengue fever patients. Site-directed mutagenesis revealed the asparagine residue at position 59 was important for epitope recognition. The 8-e epitope coincided well with the B-cell epitopes predicted by Immune Epitope Database analysis, and 3D structural modelling mapped the 8-e peptide on the surface of prM-E heterodimers. Overall, our findings characterized a linearized B-cell epitope on the pr protein of DENV, which will help to understand the life cycle of DENV and pathogenesis of dengue infections in human.

scholarly journals Identification of Continuous Human B-Cell Epitopes in the Envelope Glycoprotein of Dengue Virus Type 3 (DENV-3)

PLoS ONE ◽  
2009 ◽  
Vol 4 (10) ◽  
pp. e7425 ◽  
Author(s):  
Andréa N. M. Rangel da Silva ◽  
Eduardo J. M. Nascimento ◽  
Marli Tenório Cordeiro ◽  
Laura H. V. G. Gil ◽  
Frederico G. C. Abath ◽  
...  
Keyword(s):  
Dengue Virus ◽  
B Cell ◽  
Virus Type ◽  
Envelope Glycoprotein ◽  
B Cell Epitopes ◽  
Dengue Virus Type 3 ◽  
Type 3 ◽  
Human B Cell

scholarly journals Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Siti Naqiah Amrun ◽  
Wearn‐Xin Yee ◽  
Farhana Abu Bakar ◽  
Bernett Lee ◽  
Yiu‐Wing Kam ◽  
...  
Keyword(s):  
Dengue Virus ◽  
B Cell ◽  
Virus Infections ◽  
B Cell Epitopes ◽  
Linear B

scholarly journals In silico prediction of b-cell epitopes of dengue virus – A reverse vaccinology approach

2020 ◽  
Author(s):  
Mohan Manikandan ◽  
Shanmugaraja Prabu ◽  
Krishnan Rajeswari ◽  
Rajagopalan Kamaraj ◽  
Sundar Krishnan
Keyword(s):  
Dengue Virus ◽  
B Cell ◽  
In Silico ◽  
Reverse Vaccinology ◽  
In Silico Prediction ◽  
B Cell Epitopes
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