Enzyme-Linked Immunosorbent Assay of Antibodies to Epstein-Barr Virus Nuclear and Early Antigens in Patients with Infectious Mononucleosis and Nasopharyngeal Carcinoma

1986 ◽  
Vol 104 (3) ◽  
pp. 331 ◽  
Author(s):  
JESSICA HALPRIN
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Immunosorbent Assay ◽  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Evaluation of a Multianalyte Profiling Assay and an Enzyme-Linked Immunosorbent Assay for Serological Examination of Epstein-Barr Virus-Specific Antibody Responses in Diagnosis of Nasopharyngeal Carcinoma

2008 ◽  
Vol 15 (11) ◽  
pp. 1684-1688 ◽  
Author(s):  
Ai-Di Gu ◽  
Hao-Yuan Mo ◽  
Yan-Bo Xie ◽  
Rou-Jun Peng ◽  
Jin-Xin Bei ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Epstein Barr Virus ◽  
Antibody Responses ◽  
Nuclear Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Barr Virus ◽  
Serological Examination ◽  
Epstein Barr

ABSTRACT Assessment of antibody responses to Epstein-Barr virus (EBV) antigens has been used to assist in nasopharyngeal carcinoma (NPC) diagnosis by several methods. In this study, we evaluated an in-house Luminex multianalyte profiling (xMAP) technology and commercial enzyme-linked immunosorbent assay (ELISA) kits for serological examination of EBV-specific antibody responses in 135 NPC patients and 130 healthy controls. Four EBV biomarkers were measured: immunoglobulin A (IgA) against viral capsid antigen (VCA), EBV nuclear antigen 1 (EBNA1), diffused early antigen (EA-D), and IgG against EA-D. The sensitivities and specificities of the four markers ranged between 71.5 and 90% for xMAP assays and 80 and 92% for ELISA. Logistic regression analysis revealed that the combined markers in the xMAP assay had overall sensitivity and specificity values of 82% and 92%, respectively. The correlation coefficient (r) values for the xMAP assay and ELISA were lowest for IgA-VCA (0.468) and highest for IgA-EBNA1 (0.846); for IgA-EA-D and IgG-EA-D, the r values were 0.719 and 0.798, respectively. The concordances of the two methods for NPC discrimination were good (79 to 88%). Our results suggest that both the xMAP assay and ELISA are satisfactory for EBV antibody evaluation when multiple antigens are included.

scholarly journals Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody Responses in Nasopharyngeal Carcinoma Patients

2010 ◽  
Vol 18 (2) ◽  
pp. 298-304 ◽  
Author(s):  
E. K. Hoebe ◽  
S. H. Hutajulu ◽  
J. van Beek ◽  
S. J. Stevens ◽  
D. K. Paramita ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Humoral Immune ◽  
Barr Virus ◽  
Humoral Immune Responses ◽  
Human Sera ◽  
Epstein Barr ◽  
Humoral Responses

ABSTRACTWHO type III nasopharyngeal carcinoma (NPC) is highly prevalent in Indonesia and 100% associated with Epstein-Barr virus (EBV). NPC tumor cells express viral proteins, including BARF1, which is secreted and is considered to have oncogenic and immune-modulating properties. Recently, we found conserved mutations in the BARF1 gene in NPC isolates. This study describes the expression and purification of NPC-derived BARF1 and analyzes humoral immune responses against prototype BARF1 (B95-8) and purified native hexameric BARF1 in sera of Indonesian NPC patients (n= 155) compared to healthy EBV-positive (n= 56) and EBV-negative (n= 16) individuals. BARF1 (B95-8) expressed inEscherichia coliand baculovirus, as well as BARF1-derived peptides, did not react with IgG or IgA antibodies in NPC. Purified native hexameric BARF1 protein isolated from culture medium was used in enzyme-linked immunosorbent assay (ELISA) and revealed relatively weak IgG and IgA responses in human sera, although it had strong antibody responses to other EBV proteins. Higher IgG reactivity was found in NPC patients (P= 0.015) than in regional Indonesian controls or EBV-negative individuals (P< 0.001). IgA responses to native BARF1 were marginal. NPC sera with the highest IgG responses to hexameric BARF1 in ELISA showed detectable reactivity with denatured BARF1 by immunoblotting. In conclusion, BARF1 has low immunogenicity for humoral responses and requires native conformation for antibody binding. The presence of antibodies against native BARF1 in the blood of NPC patients provides evidence that the protein is expressed and secreted as a hexameric protein in NPC patients.

Enzyme-linked immunosorbent assay for the detection of Epstein-Barr Virus-Induced Antigens and antibodies

1983 ◽  
Vol 63 (2) ◽  
pp. 171-185 ◽  
Author(s):  
Lars Sternås ◽  
Janos Luka ◽  
Bengt Kallin ◽  
Anders Rosén ◽  
Werner Henle ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Epstein Barr Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Barr Virus ◽  
Epstein Barr

Detection of Antibodies to Epstein-Barr Virus Antigens by Enzyme-Linked Immunosorbent Assay

1982 ◽  
Vol 146 (6) ◽  
pp. 734-740 ◽  
Author(s):  
Ralph F. Hopkins ◽  
Tracy J. Witmer ◽  
Russell H. Neubauer ◽  
Harvey Rabin
Keyword(s):  
Immunosorbent Assay ◽  
Epstein Barr Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Barr Virus ◽  
Virus Antigens ◽  
Epstein Barr

scholarly journals Development and Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Based on the 18-Kilodalton Matrix Protein for Diagnosis of Primary EBV Infection

1998 ◽  
Vol 36 (11) ◽  
pp. 3359-3361 ◽  
Author(s):  
K. H. Chan ◽  
R. X. Luo ◽  
H. L. Chen ◽  
M. H. Ng ◽  
W. H. Seto ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Epstein Barr Virus ◽  
Matrix Protein ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Clinical Specimens ◽  
Barr Virus ◽  
Ebv Infection ◽  
Igm Elisa ◽  
Epstein Barr

A new immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) based on the recombinant Epstein-Barr virus (EBV) matrix protein was developed. Compared to indirect immunofluorescence for the detection of IgM antibody to the EBV capsid antigen on clinical specimens, the sensitivity and specificity of the new IgM ELISA were 96 and 96%, respectively.

scholarly journals Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Using a Synthetic Convergent Peptide Library, or Mixotope, for Diagnosis of Primary EBV Infection

1999 ◽  
Vol 37 (7) ◽  
pp. 2366-2368 ◽  
Author(s):  
D. Tranchand-Bunel ◽  
H. Gras-Masse ◽  
B. Bourez ◽  
L. Dedecker ◽  
C. Auriault
Keyword(s):  
Immunosorbent Assay ◽  
Epstein Barr Virus ◽  
Peptide Library ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Capsid Antigen ◽  
Amino Acid Peptide ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

An immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) was developed by using a 24-amino-acid peptide of the 18-kDa Epstein-Barr virus (EBV) viral capsid antigen (VCAp18). IgM detection was increased by 23% by using this antigen. Detection of IgM antibodies to the EBV proteins in the new ELISA was 100% specific and 95% sensitive.

False Positive EBNA IgM and IgG Antibody Tests for Infectious Mononucleosis in Children

PEDIATRICS ◽  
1994 ◽  
Vol 94 (6) ◽  
pp. 892-894 ◽  
Author(s):  
Dorothy Levine ◽  
Rosemary Klenk ◽  
Alan Morelli ◽  
Nancy Hofreuter ◽  
Richard C. Tilton ◽  
...  
Keyword(s):  
Virus Infection ◽  
Infectious Mononucleosis ◽  
False Positive ◽  
Epstein Barr Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epstein Barr Virus Infection ◽  
Igg Antibody ◽  
Barr Virus ◽  
Epstein Barr ◽  
Barr Virus Infection

Objectives. To assess the reliability of the Monolert test, a new enzyme-linked immunosorbent assay for the diagnosis of acute infectious mononucleosis (IM). Design. A retrospective laboratory and clinical analysis of 38 children diagnosed with acute IM. Setting. A suburban pediatric practice in Connecticut. Patients. Thirty-eight children (ages 18 months to 17 years) who were diagnosed with acute IM using the Monolert test during the period October 1992 to August 1993. Results. Eighty-three percent of these children had no evidence of Epstein-Barr virus infection on subsequent investigation. The false positive results of the Monolert test could not be explained on the basis of elevated antibody titers to either cytomegalovirus or Borrelia burgdorferi. Conclusion. Monolert is a poor screening test and is of little apparent value as a diagnostic test for acute Epstein-Barr virus infection in pediatric patients.

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