scholarly journals The Lyme Disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Xiaotian Tang ◽  
Yongguo Cao ◽  
Gunjan Arora ◽  
Jesse Hwang ◽  
Andaleeb Sajid ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Ixodes Scapularis ◽  
Phospholipid Metabolism ◽  
Adiponectin Receptor ◽  
Rna Seq ◽  
Alternative Pathways ◽  
Phosphatidylserine Synthase ◽  
Disease Agent ◽  
Complement C1q ◽  
Lyme Disease Agent

Adiponectin-mediated pathways contribute to mammalian homeostasis; however, little is known about adiponectin and adiponectin receptor signaling in arthropods. In this study, we demonstrate that Ixodes scapularis ticks have an adiponectin receptor-like protein (ISARL) but lack adiponectin - suggesting activation by alternative pathways. ISARL expression is significantly upregulated in the tick gut after Borrelia burgdorferi infection suggesting that ISARL-signaling may be co-opted by the Lyme disease agent. Consistent with this, RNA interference (RNAi)-mediated silencing of ISARL significantly reduced the B. burgdorferi burden in the tick. RNA-seq-based transcriptomics and RNAi assays demonstrate that ISARL-mediated phospholipid metabolism by phosphatidylserine synthase I is associated with B. burgdorferi survival. Furthermore, the tick complement C1q-like protein 3 interacts with ISARL, and B. burgdorferi facilitates this process. This study identifies a new tick metabolic pathway that is connected to the life cycle of the Lyme disease spirochete.

scholarly journals The Lyme Disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector

2021 ◽  
Author(s):  
Xiaotian Tang ◽  
yongguo cao ◽  
Gunjan Arora ◽  
Jesse Hwang ◽  
Andaleeb Sajid ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Ixodes Scapularis ◽  
Phospholipid Metabolism ◽  
Adiponectin Receptor ◽  
Rna Seq ◽  
Alternative Pathways ◽  
Phosphatidylserine Synthase ◽  
Disease Agent ◽  
Complement C1q ◽  
Lyme Disease Agent

Adiponectin-mediated pathways contribute to mammalian homeostasis; however, little is known about adiponectin and adiponectin receptor signaling in arthropods. In this study, we demonstrate that Ixodes scapularis ticks have an adiponectin receptor-like protein (ISARL) but lack adiponectin – suggesting activation by alternative pathways. ISARL expression is significantly upregulated in the tick gut after Borrelia burgdorferi infection suggesting that ISARL-signaling may be co-opted by the Lyme disease agent. Consistent with this, RNA interference (RNAi)-mediated silencing of ISARL significantly reduced the B. burgdorferi burden in the tick. RNA-seq-based transcriptomics and RNAi assays demonstrate that ISARL-mediated phospholipid metabolism by phosphatidylserine synthase I is associated with B. burgdorferi survival. Furthermore, the tick complement C1q-like protein 3 interacts with ISARL, and B. burgdorferi facilitates this process. This study identifies a new tick metabolic pathway that is connected to the life cycle of the Lyme disease spirochete.

scholarly journals Tick Histamine Release Factor Is Critical for Ixodes scapularis Engorgement and Transmission of the Lyme Disease Agent

2010 ◽  
Vol 6 (11) ◽  
pp. e1001205 ◽  
Author(s):  
Jianfeng Dai ◽  
Sukanya Narasimhan ◽  
Lili Zhang ◽  
Lei Liu ◽  
Penghua Wang ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Histamine Release ◽  
Ixodes Scapularis ◽  
Release Factor ◽  
Disease Agent ◽  
Lyme Disease Agent

scholarly journals Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Karie E. Robertson ◽  
Chloe D. Truong ◽  
Felicia M. Craciunescu ◽  
Jay-How Yang ◽  
Po-Lin Chiu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Virulence Factors ◽  
Protein Complexes ◽  
Signal Sequence ◽  
Surface Proteins ◽  
Disease Agent ◽  
And Function ◽  
Lyme Disease Agent

AbstractMembrane-embedded proteins are critical to the establishment, survival and persistence in the host of the Lyme disease bacterium Borrelia burgdorferi (Bb), but to date, there are no solved structures of transmembrane proteins representing these attractive therapeutic targets. All available structures from the genus Borrelia represent proteins expressed without a membrane-targeting signal peptide, thus avoiding conserved pathways that modify, fold and assemble membrane protein complexes. Towards elucidating structure and function of these critical proteins, we directed translocation of eleven expression-optimized Bb virulence factors, including the signal sequence, to the Escherichia coli membrane, of which five, BBA57, HtrA, BB0238, BB0323, and DipA, were expressed with C-terminal His-tags. P66 was also expressed using the PelB signal sequence fused to maltose binding protein. Membrane-associated BBA57 lipoprotein was solubilized by non-ionic and zwitterionic detergents. We show BBA57 translocation to the outer membrane, purification at a level sufficient for structural studies, and evidence for an α-helical multimer. Previous studies showed multiple critical roles of BBA57 in transmission, joint arthritis, carditis, weakening immune responses, and regulating other Bb outer surface proteins. In describing the first purification of membrane-translocated BBA57, this work will support subsequent studies that reveal the precise mechanisms of this important Lyme disease virulence factor.

scholarly journals Plasmid diversity and phylogenetic consistency in the Lyme disease agent Borrelia burgdorferi

BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Sherwood R. Casjens ◽  
Eddie B. Gilcrease ◽  
Marija Vujadinovic ◽  
Emmanuel F. Mongodin ◽  
Benjamin J. Luft ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Disease Agent ◽  
Lyme Disease Agent ◽  
Plasmid Diversity

scholarly journals Amblyomma americanum (Acari: Ixodidae) Ticks Are Not Vectors of the Lyme Disease Agent, Borrelia burgdorferi (Spirocheatales: Spirochaetaceae): A Review of the Evidence

2018 ◽  
Vol 55 (3) ◽  
pp. 501-514 ◽  
Author(s):  
Ellen Y Stromdahl ◽  
Robyn M Nadolny ◽  
Graham J Hickling ◽  
Sarah A Hamer ◽  
Nicholas H Ogden ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Amblyomma Americanum ◽  
Disease Agent ◽  
Lyme Disease Agent
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