Isolation and Characterization of Lactones from Ethanolic Extract of Leaves of Naravelia Zeylanica

In present study two chemical constituents viz. Flavon 5,4’ dimethoxy 8 methyl 7-O- β -D glucopyranoside-5’- β -D-glucopyranoside and xanthone 3-acetoxy-1-hydroxy-6-methoxy8-O– β –D-glucopyranosyl-(1 → 3)- α –L- rhamnopyranoside from the ethanolic extract of flowers of Bombex ceiba have been isolated and characterized.

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ISOLATION AND CHARACTERIZATION OF GALLIC ACID FROM THE ETHANOLIC EXTRACT OF LEAVES OF BUTEA MONOSPERMA (LAMB)

International Research Journal of Pharmacy ◽

10.7897/2230-8407.100253 ◽

2019 ◽

Vol 10 (2) ◽

pp. 114-117

Author(s):

J Banu Rekha ◽

M Kumar ◽

B Jaykar

Keyword(s):

Gallic Acid ◽

Ethanolic Extract ◽

Butea Monosperma ◽

Isolation And Characterization

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ISOLATION AND CHARACTERIZATION OF N-HEPTACOSANYL OLEATE FROM THE WHOLE AERIAL PARTS OF CENTELLA ASIATICA LINN.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9s3.13427 ◽

2016 ◽

Vol 9 (9) ◽

pp. 23

Author(s):

Rashmi Saxena Pal ◽

Yogendra Pal ◽

Pranay Wal ◽

Vijender Singh

Keyword(s):

Fast Atom Bombardment ◽

Ethanolic Extract ◽

Centella Asiatica ◽

Phytochemical Study ◽

Aerial Parts ◽

Isolation And Characterization ◽

Soxhlet Apparatus ◽

1H Nuclear Magnetic Resonance ◽

Spectral Data Analysis

ABSTRACTVarious studies have already been performed involving the whole aerial parts of Centella asiatica (L.) (Umbelliferae), commonly known as gotu kola orjalbrahmi, and thus, the present investigation has been carried out for the phytochemical study of an ethanolic extract of the aerial parts of C. asiatica.To perform this activity, the drug (1.5 kg) was exhaustively extracted in 95% ethanol using Soxhlet apparatus. The column chromatography wasperformed then for isolating the various phytoconstituents using the solvents of increasing polarity from petroleum ether to methanol. The isolatedcompounds were structurally elucidated using various spectral data analysis, i.e. infrared,1H nuclear magnetic resonance (NMR),13C NMR, and positiveion fast atom bombardment mass spectrometry. One of the isolated compounds was characterized as n-heptacosanyl oleate.Keywords: Whole aerial parts, Centella asiatica, Soxhlet, Column, Heptacosanyl oleate.

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Isolation and Characterization of Nuciferoic Acid, a Novel Keto Fatty Acid with Hyaluronidase Inhibitory Activity from Cocos nucifera Linn. Endocarp

Current Topics in Medicinal Chemistry ◽

10.2174/1568026619666181224111319 ◽

2019 ◽

Vol 18 (27) ◽

pp. 2367-2378

Author(s):

Rajeev K. Singla ◽

Mohammed Ali ◽

Mohammad A. Kamal ◽

Ashok K. Dubey

Keyword(s):

Fatty Acid ◽

Cocos Nucifera ◽

Ethanolic Extract ◽

Isolation And Characterization ◽

Hard Shell ◽

Anti Inflammatory ◽

Key Sites ◽

Inhibitory Potential ◽

Sites Of Action

Background: Inflammation and oxidative stress are very closely related to pathophysiological processes and linked to multiple chronic diseases. Traditionally, the coconut fruits were used in Guatemala for treatment of dermatitis and inflammation. Isolation of the anti-inflammatory agent from the hard shell of the coconut fruit was targeted in the current study. Methods: Fractionation of ethanolic extract of the coconut hard shell was done by using column chromatography, solvent treatments and TLC that led to the isolation of a molecule. Results and Discussion: Spectral characterization of the molecule by LC-MS/MS QTOF, FTIR, 1HNMR, 13C-NMR, HMQC and HMBC indicated that it is a novel keto fatty acid, which is named as nuciferoic acid. Hyaluronidase inhibitory potential of the nuciferoic acid was found to be moderate. It was further docked in all the ten cavities of hyaluronidase and was compared with the substrate hyaluronic acid. Cavity 1 and cavity 4 could be the probable sites of action on hyaluronidase for nuciferoic acid. ADME and toxicological characterization suggested that the key sites of metabolism on nuciferoic acid are C1, C2, C14 and C17. Toxicity prediction against 55 toxicological endpoints revealed that nuciferoic acid does not have any indication of existing toxicological features. Conclusion: A novel keto fatty acid, nuciferoic acid, from C. nucifera hard shell has been isolated and characterized. It was found to inhibit hyaluronidase activity, which indicated its potential application as an anti-inflammatory drug or as an adjuvant.

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