scholarly journals Ixodes tropicalis (Acari: Ixodidae) infesting a human and molecular detection of Rickettsia bellii, Colombia

Biomédica ◽  
2021 ◽  
Vol 41 (2) ◽  
pp. 347-352
Author(s):  
Juan Carlos Quintero ◽  
María L. Félix ◽  
José M. Venzal ◽  
Santiago Nava
Keyword(s):  
16S Rrna ◽  
Metropolitan Area ◽  
Tick Species ◽  
Molecular Detection ◽  
Wild Rodents ◽  
First Report ◽  
Glta Gene ◽  
Rickettsia Bellii ◽  
Rrna Sequences ◽  
16S Rrna Sequences

Introduction: Ixodes tropicalis is a little-known tick species reported parasitizing wild rodents only in Colombia and Perú.Objective: To report a case of I. tropicalis infesting a human in the south of the metropolitan area of the Valle de Aburrá, Antioquia, Colombia, and to report the molecular detection of Rickettsia bellii in this species.Materials and methods: The tick was identified using a morphological key and sequencing of tick mitochondrial 16S rRNA. Additionally, bacterial and protozoa pathogens were evaluated using PCR for the detection of Rickettsia spp., family Anaplasmataceae, Borrelia spp., and piroplasmid.Results: We identified the tick as an I. tropicalis female according to Kohls, 1956, description and to partial 16S rRNA sequences showing a minimum of 5% divergencies compared to Ixodes sequences. We also detected the gltA gene of R. bellii in the tick with 99.87% of identity.Conclusion: This is the first report in Colombia of a species of the Ixodes genus parasitizing a human and the first report of the detection of R. bellii in this tick species.

scholarly journals First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Manal A. Gumaa ◽  
Abeer Babiker Idris ◽  
N. E. Bilal ◽  
Mohamed A. Hassan
Keyword(s):  
Staphylococcus Aureus ◽  
16S Rrna ◽  
Ribosomal Rna ◽  
Molecular Basis ◽  
Molecular Detection ◽  
Rrna Gene ◽  
Wound Infections ◽  
Resistant Phenotype ◽  
Rrna Sequences ◽  
16S Rrna Sequences

Abstract Objective In this study, we analyzed the molecular evolution of Staphylococcus aureus isolates using 16S rRNA gene and phylogenetic analysis to detect the prevalence of S. aureus infections in Sudan. Results Molecular detection of S. aureus has shown that 20 (43.47%) of patients were positive for S. aureus. The phylogenetic tree of 16S rRNA sequences was divided into three lineages of S. aureus isolates detected from wound infections in Sudan. Nucleotides base-pair substitution was appeared at position 249. This mutation do not linked with Macrolides, Lincosamides and Streptogramines b resistant phenotype. Further studies should investigate the effect of that mutation on resistance to other antibiotics.

scholarly journals First insights into Molecular basis Identification of 16s Ribosomal RNA Gene of Staphylococcus aureus Isolated from Sudan

2021 ◽  
Author(s):  
Manal Abdalla Gumaa ◽  
Abeer Babiker Idris ◽  
Nasr aldin Bilal Mohamed ◽  
Mohamed Ahmed Hassan
Keyword(s):  
Staphylococcus Aureus ◽  
16S Rrna ◽  
Ribosomal Rna ◽  
Molecular Detection ◽  
Rrna Gene ◽  
Wound Infections ◽  
Resistant Phenotype ◽  
16S Ribosomal Rna Gene ◽  
Rrna Sequences ◽  
16S Rrna Sequences

Abstract Objective: In this study, we analyzed the molecular evolution of Staphylococcus aureus isolates using 16S rRNA gene and phylogenetic analysis to detect the prevalence of S. aureus infections in Sudan. Results: Molecular detection of S.aureus has shown that 20(43.47%) of patients were positive for S.aureus. The phylogenetic tree of 16S rRNA sequences was divided into three lineages of S.aureus isolates detected from wound infections in Sudan. Nucleotides base-pair substitution was appeared at position 249. This mutation do not linked with Macrolides, Lincosamides and Streptogramines b resistant phenotype. Further studies should investigate the effect of that mutation on resistance to other antibiotics.

scholarly journals Correction to: Bacterial community assemblages in classroom floor dust of 50 public schools in a large city: characterization using 16S rRNA sequences and associations with environmental factors

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Ju-Hyeong Park ◽  
Angela R. Lemons ◽  
Jerry Roseman ◽  
Brett J. Green ◽  
Jean M. Cox-Ganser
Keyword(s):  
Public Schools ◽  
Environmental Factors ◽  
Bacterial Community ◽  
16S Rrna ◽  
Large City ◽  
Floor Dust ◽  
Community Assemblages ◽  
Rrna Sequences ◽  
16S Rrna Sequences

An amendment to this paper has been published and can be accessed via the original article.

scholarly journals 16S rRNA sequences reveal uncultured inhabitants of a well-studied thermal community

1990 ◽  
Vol 75 (2-3) ◽  
pp. 105-115 ◽  
Author(s):  
David M. Ward ◽  
Roland Weller ◽  
Mary M. Bateson
Keyword(s):  
16S Rrna ◽  
Rrna Sequences ◽  
16S Rrna Sequences

scholarly journals Divergence and Redundancy of 16S rRNA Sequences in Genomes with Multiple rrn Operons

2004 ◽  
Vol 186 (9) ◽  
pp. 2629-2635 ◽  
Author(s):  
Silvia G. Acinas ◽  
Luisa A. Marcelino ◽  
Vanja Klepac-Ceraj ◽  
Martin F. Polz
Keyword(s):  
16S Rrna ◽  
Thermophilic Bacteria ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Bacterial Genomes ◽  
16S Rrna Sequence ◽  
Thermoanaerobacter Tengcongensis ◽  
Copy Numbers ◽  
Rrna Sequences ◽  
16S Rrna Sequences

ABSTRACT The level of sequence heterogeneity among rrn operons within genomes determines the accuracy of diversity estimation by 16S rRNA-based methods. Furthermore, the occurrence of widespread horizontal gene transfer (HGT) between distantly related rrn operons casts doubt on reconstructions of phylogenetic relationships. For this study, patterns of distribution of rrn copy numbers, interoperonic divergence, and redundancy of 16S rRNA sequences were evaluated. Bacterial genomes display up to 15 operons and operon numbers up to 7 are commonly found, but ∼40% of the organisms analyzed have either one or two operons. Among the Archaea, a single operon appears to dominate and the highest number of operons is five. About 40% of sequences among 380 operons in 76 bacterial genomes with multiple operons were identical to at least one other 16S rRNA sequence in the same genome, and in 38% of the genomes all 16S rRNAs were invariant. For Archaea, the number of identical operons was only 25%, but only five genomes with 21 operons are currently available. These considerations suggest an upper bound of roughly threefold overestimation of bacterial diversity resulting from cloning and sequencing of 16S rRNA genes from the environment; however, the inclusion of genomes with a single rrn operon may lower this correction factor to ∼2.5. Divergence among operons appears to be small overall for both Bacteria and Archaea, with the vast majority of 16S rRNA sequences showing <1% nucleotide differences. Only five genomes with operons with a higher level of nucleotide divergence were detected, and Thermoanaerobacter tengcongensis exhibited the highest level of divergence (11.6%) noted to date. Overall, four of the five extreme cases of operon differences occurred among thermophilic bacteria, suggesting a much higher incidence of HGT in these bacteria than in other groups.

scholarly journals Occurrence of diazotrophic bacteria in Araucaria angustifolia

2007 ◽  
Vol 64 (3) ◽  
pp. 303-304 ◽  
Author(s):  
Rafaela de Fátima Neroni ◽  
Elke Jurandy Bran Nogueira Cardoso
Keyword(s):  
16S Rrna ◽  
Acetylene Reduction ◽  
Araucaria Angustifolia ◽  
Diazotrophic Bacteria ◽  
Araucaria Forest ◽  
Solid Media ◽  
Reduction Assay ◽  
Semi Solid ◽  
Rrna Sequences ◽  
16S Rrna Sequences

Araucaria angustifolia is an environmentally threatened tree and the whole biota of the Araucaria Forest should be investigated with the aim of its preservation. Diazotrophic bacteria are extremely important for the maintenance of ecosystems, but they have never been studied in Araucaria Forests. In this study, diazotrophic bacteria were isolated from Araucaria roots and soil, when grown in semi-specific, semi-solid media. The diazotrophic character of some recovered isolates could be confirmed using the acetylene reduction assay. According to their 16S rRNA sequences, most of these isolates belong to the genus Burkholderia.

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