The landscape of tumors-infiltrate immune cells in papillary thyroid carcinoma and its prognostic value

Introduction Thyroid cancer is a very common malignant tumor in the endocrine system, while the incidence of papillary thyroid carcinoma (PTC) throughout the world also shows a trend of increase year by year. In this study, we constructed two models: ICIscore and Riskscore. Combined with these two models, we can make more accurate and reasonable inferences about the prognosis of PTC patients. Methods We selected 481 PTC samples from TCGA and 147 PTC samples from GEO (49 samples in GSE33630, 65 samples in GSE35570 and 33 samples in GSE60542). We performed consistent clustering for them and divided them into three subgroups and screened differentially expressed genes from these three subgroups. Then we divided the differential genes into three subtypes. We also distinguished the up-regulated and down-regulated genes and calculated ICIscore for each PTC sample. ICIscore consists of two parts: (1) the PCAu was calculated from up-regulated genes. (2) the PCAd was calculated from down-regulated genes. The PCAu and PCAd of each sample were the first principal component of the relevant gene. What’s more, we divided the patients into two groups and constructed mRNA prognostic signatures. Additionally we also verified the independent prognostic value of the signature. Results Though ICIscore, we were able to observe the relationship between immune infiltration and prognosis. The result suggests that the activation of the immune system may have both positive and negative consequences. Though Riskscore, we could make more accurate predictions about the prognosis of patients with PTC. Meanwhile, we also generated and validated the ICIscore group and Riskscore group respectively. Conclusion All the research results show that by combining the two models constructed, ICIscore and Riskscore, we can make a more accurate and reasonable inference about the prognosis of patients with clinical PTC patients. This suggests that we can provide more effective and reasonable treatment plan for clinical PTC patients.

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Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma

International Journal of Molecular Sciences ◽

10.3390/ijms22136726 ◽

2021 ◽

Vol 22 (13) ◽

pp. 6726

Author(s):

Agata M. Gaweł ◽

Maciej Ratajczak ◽

Ewa Gajda ◽

Małgorzata Grzanka ◽

Agnieszka Paziewska ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Endocrine System ◽

Metastatic Potential ◽

Multidrug Resistant ◽

Thyroid Tumor ◽

Braf V600e ◽

Papillary Thyroid ◽

Spheroid Formation

Background: Thyroid carcinoma (TC) is the most common endocrine system malignancy, and papillary thyroid carcinoma (PTC) accounts for >80% of all TC cases. Nevertheless, PTC pathogenesis is still not fully understood. The aim of the study was to elucidate the role of the FRMD5 protein in the regulation of biological pathways associated with the development of PTC. We imply that the presence of certain genetic aberrations (e.g., BRAF V600E mutation) is associated with the activity of FRMD5. Methods: The studies were conducted on TPC1 and BCPAP (BRAF V600E) model PTC-derived cells. Transfection with siRNA was used to deplete the expression of FRMD5. The mRNA expression and protein yield were evaluated using RT-qPCR and Western blot techniques. Proliferation, migration, invasiveness, adhesion, spheroid formation, and survival tests were performed. RNA sequencing and phospho-kinase proteome profiling were used to assess signaling pathways associated with the FRMD5 expressional status. Results: The obtained data indicate that the expression of FRMD5 is significantly enhanced in BRAF V600E tumor specimens and cells. It was observed that a drop in intracellular yield of FRMD5 results in significant alternations in the migration, invasiveness, adhesion, and spheroid formation potential of PTC-derived cells. Importantly, significant divergences in the effect of FRMD5 depletion in both BRAF-wt and BRAF-mutated PTC cells were observed. It was also found that knockdown of FRMD5 significantly alters the expression of multidrug resistant genes. Conclusions: This is the first report highlighting the importance of the FRMD5 protein in the biology of PTCs. The results suggest that the FRMD5 protein can play an important role in controlling the metastatic potential and multidrug resistance of thyroid tumor cells.

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Prognostic value of morphometry in papillary thyroid carcinoma

Human Pathology ◽

10.1016/0046-8177(89)90126-3 ◽

1989 ◽

Vol 20 (3) ◽

pp. 215-218 ◽

Cited By ~ 25

Author(s):

Robert A. Ambros ◽

Ronald C. Trost ◽

Alkis Y. Campbell ◽

W. Clark Lambert

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Prognostic Value ◽

Papillary Thyroid

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Prognostic value of poorly differentiated carcinoma in Japanese Society of Thyroid Surgery in a series of papillary thyroid carcinoma patients: Comparison with risk classification system in Kuma Hospital

Endocrine Journal ◽

10.1507/endocrj.ej12-0175 ◽

2012 ◽

Vol 59 (9) ◽

pp. 817-821 ◽

Cited By ~ 1

Author(s):

Yasuhiro Ito ◽

Mitsuyoshi Hirokawa ◽

Minoru Kihara ◽

Yuuki Takamura ◽

Kaoru Kobayashi ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Thyroid Surgery ◽

Classification System ◽

Prognostic Value ◽

Japanese Society ◽

Risk Classification ◽

Papillary Thyroid ◽

Poorly Differentiated Carcinoma ◽

Poorly Differentiated

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Association between Thyroiditis and Multifocality in Papillary Thyroid Carcinoma

International Archives of Otorhinolaryngology ◽

10.1055/s-0040-1710307 ◽

2020 ◽

Author(s):

Rogério Aparecido Dedivitis ◽

Leandro Luongo de Matos ◽

Felipe Guilherme Silva Souza ◽

Jose Luis Bogado Ortiz

Keyword(s):

Retrospective Study ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Total Thyroidectomy ◽

Direct Relationship ◽

Statistical Significance ◽

Hashimoto Thyroiditis ◽

Papillary Thyroid ◽

Multifocal Lesions ◽

The Relationship

Abstract Introduction Hashimoto thyroiditis (HT) shares many characteristics with papillary thyroid carcinoma (PTC), and some studies show that, when associated, PTC is diagnosed mostly with smaller lesions and multifocal pattern. Objective To evaluate the relationship between HT and PTC. Methods A retrospective study of 155 patients who underwent total thyroidectomy from 2009 to 2015. Demographical, clinical and ultrasonographical data, as well as anatomopathological findings were evaluated. Results There were signs of thyroidits in 35 patients, and 114 patients had a unifocal disease. There was no statistical significance between the variables studied and thyroiditis. However, when compared with the occurrence of unifocal or multifocal lesions, there was statistical significance regarding age (p = 0.038) and mass (p = 0.031). There was no direct relationship between thyroiditis and multifocality (p = 0.325) nor between thyroiditis and cervical extension of the disease (p = 0.300 e p = 0.434). Conclusion There was no relationship between thyroiditis and multifocality in cases of PTC.

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Abstract 460: Diagnostic and prognostic value of expression of CCND1 splice variant cyclin D1b in papillary thyroid carcinoma

10.1158/1538-7445.am2016-460 ◽

2016 ◽

Author(s):

Chan Kwon Jung ◽

Sora Jeon ◽

Yourha Kim ◽

Ahwon Lee ◽

Gyeong Sin Park

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Splice Variant ◽

Prognostic Value ◽

Papillary Thyroid ◽

Diagnostic And Prognostic Value

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BRAFK601E Mutation in a Follicular Thyroid Adenoma: A Case Report

International Journal of Surgical Pathology ◽

10.1177/1066896916688083 ◽

2017 ◽

Vol 25 (4) ◽

pp. 348-351 ◽

Cited By ~ 9

Author(s):

Elisabetta Macerola ◽

Liborio Torregrossa ◽

Clara Ugolini ◽

Sohail Bakkar ◽

Paolo Vitti ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Treatment Plan ◽

Follicular Adenoma ◽

Surrounding Tissue ◽

Papillary Thyroid ◽

Follicular Variant ◽

Thyroid Lesion ◽

Cytological Smear ◽

Benign Thyroid

BRAF mutations represent the most common genetic alteration in papillary thyroid carcinoma (PTC). The p.V600E mutation is specific for the classic and tall-cell variants of PTC and has been associated with a more aggressive biologic behavior. On the other hand, the p.K601E mutation is peculiar to the follicular variant of PTC, and seems to be a favorable prognostic indicator. A 12-year-old boy presented with a 10-mm left-sided thyroid nodule. Fine-needle aspiration cytology reported the lesion as suspicious for a follicular neoplasm (Bethesda category IV). The patient underwent lobectomy, and histopathology revealed a follicular adenoma with normal surrounding tissue. The cytological smear was found to be positive for BRAF p.K601E mutation, and this was later confirmed on the corresponding paraffin block. This case was independently revised by 4 expert pathologists, all of whom confirmed the benign nature of the thyroid lesion. This article describes the presence of a BRAF mutation in a benign thyroid lesion. To the authors’ knowledge, this is the fourth case of follicular adenoma carrying BRAFK601E reported in literature to date. BRAFK601E mutation can occur in benign thyroid lesions. This finding, in the context of the current literature and the recently proposed reclassification of the noninvasive encapsulated follicular variant of papillary thyroid carcinoma into a benign lesion, confirms the importance of preoperative BRAF p.K601E testing in offering patients a tailored treatment plan and avoiding overtreatment.

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Evaluation of survivin expression and its prognostic value in papillary thyroid carcinoma

Pathology - Research and Practice ◽

10.1016/j.prp.2013.09.014 ◽

2014 ◽

Vol 210 (1) ◽

pp. 30-34 ◽

Cited By ~ 9

Author(s):

Sonja Selemetjev ◽

Tijana Isic Dencic ◽

Ilona Marecko ◽

Jelena Jankovic ◽

Ivan Paunovic ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Prognostic Value ◽

Survivin Expression ◽

Papillary Thyroid

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Metastasis of follicular variant of papillary thyroid carcinoma masquerading as primary temporal bone tumour

The Journal of Laryngology & Otology ◽

10.1017/s0022215110002926 ◽

2011 ◽

Vol 125 (5) ◽

pp. 528-532 ◽

Cited By ~ 6

Author(s):

S C Hugh ◽

D Enepekides ◽

J Wong ◽

R Yeung ◽

V Y W Lin

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Bone Mass ◽

Temporal Bone ◽

Treatment Plan ◽

Correct Diagnosis ◽

Bone Tumour ◽

Final Diagnosis ◽

Papillary Thyroid ◽

Follicular Variant

AbstractObjective:We describe the first published case of papillary thyroid carcinoma metastatic to the temporal bone.Case report:A 64-year-old woman presented with a large left temporal bone mass centred in the jugular foramen, initially thought to be a paraganglioma or schwannoma. She was simultaneously being investigated for a left-sided thyroid nodule, which was found to be unremarkable on repeated fine needle aspiration cytology. A biopsy of the temporal bone mass indicated that it was of thyroid origin. The patient underwent total thyroidectomy, which enabled a final diagnosis of follicular-variant papillary thyroid carcinoma with metastasis to the temporal bone.Conclusion:Although biopsy is not the usual management for many types of temporal bone mass, pathological investigation is recommended if the tumour has an atypical growth rate, location, spread and/or radiological features. Metastasis of papillary thyroid carcinoma to the skull base is extremely rare, and correct diagnosis is essential in order to pursue an effective treatment plan.

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