scholarly journals An epidemiological investigation of porcine circovirus type 2 and porcine circovirus type 3 infections in Tianjin, North China

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9735
Author(s):  
Shuai-Yong Wang ◽  
Ying-Feng Sun ◽  
Qi Wang ◽  
Ling-Xue Yu ◽  
Shi-Qiang Zhu ◽  
...  
Keyword(s):  
North China ◽  
Clinical Symptoms ◽  
The United States ◽  
Porcine Circovirus Type ◽  
Postweaning Multisystemic Wasting Syndrome ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Tissue Samples ◽  
Type 3

Novel porcine circovirus type 3 (PCV3), first identified in the United States, has been detected in many other countries. Porcine circovirus is associated with postweaning multisystemic wasting syndrome, reproductive failure, congenital tremors, and other clinical symptoms. In this study, we established a double polymerase chain reaction assay for detecting both porcine circovirus type 2 (PCV2) and PCV3. This is the first study to detect and characterize the PCV3 genome in the Tianjin region of North China. We collected a total of 169 tissue samples from seven farms between 2016 and 2018. The PCV3-positive rate of all tissue samples was 37.3% (63/169) and the rate of PCV2 and PCV3 coinfection was 14.8% (25/169). PCV2 and PCV3 coinfections with more serious clinical symptoms were found in only three farms. We sequenced three PCV3 strains selected from tissue samples that were positively identified. The complete genome sequences of the three strains shared 97.6–99.4% nucleotide identities with the PCV3 strains in GenBank. Our results showed the extent of PCV3’s spread in Tianjin, and the need to further study PCV3’s pathobiology, epidemiology, isolation, and coinfection.

scholarly journals Experimental Reproduction of Postweaning Multisystemic Wasting Syndrome in Cesarean-Derived, Colostrum-Deprived Piglets Inoculated with Porcine Circovirus Type 2 (PCV2): Investigation of Quantitative PCV2 Distribution and Antibody Responses

2003 ◽  
Vol 15 (2) ◽  
pp. 107-114 ◽  
Author(s):  
Y. Okuda ◽  
M. Ono ◽  
S. Yazawa ◽  
I. Shibata
Keyword(s):  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Postweaning Multisystemic Wasting Syndrome ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Control Group ◽  
Serum Samples ◽  
Tissue Samples ◽  
Wasting Syndrome

Sixteen cesarean-derived, colostrum-deprived piglets were inoculated intranasally with porcine circovirus type 2 (PCV2), originally isolated from a pig affected with postweaning multisystemic wasting syndrome (PMWS). At 1 day postinoculation (PI), 3 of the 5 piglets in the uninoculated control group were moved to the room of inoculated piglets for contact exposure. Porcine circovirus type 2 was detected by polymerase chain reaction (PCR) in swabs from inoculated piglets from 1 day PI and from contact piglets from 2 days after cohabitation. Porcine circovirus type 2 was also detected in all serum samples but not in control piglets 7 days PI. Until the end of study, PCV2 was detected in swabs and serum samples by PCR but not in the control piglets. One inoculated piglet died suddenly without clinical signs 19 days PI. Beginning at 14 days PI, 5 piglets, including 1 contact piglet, had clinical signs of depression, anorexia, and icterus, and 1 inoculated piglet died 21 days PI. Most of the piglets exhibiting the above clinical signs became moribund and were necropsied 21 and 28 days PI. In the piglets that showed clinical signs, gross lesions, including icterus of liver and hemorrhage in stomach, and typical histopathological lesions of PMWS, such as lymphoid depletion and basophilic intracytoplasmic inclusion bodies in lymph nodes and other tissues, were observed. Porcine circovirus type 2 was detected by PCR in all tissue samples except in those of the control piglets. Porcine circovirus type 2 was recovered from several tissue samples of the piglets necropsied until 35 days PI. In particular, PCV2 was recovered in high titer from most of the tissue samples of the piglets exhibiting clinical signs. Serum antibody against PCV2 was mostly detected in inoculated piglets and in contact piglets 14 and 21 days PI by an indirect fluorescence antibody test but was not detected in the piglets exhibiting clinical signs until 28 days PI. These results indicate that PCV2 was able to induce clinical PMWS in the absence of other swine pathogens and that there were significant differences in both the quantitative PCV2 distribution in tissues and the antibody response between the piglets that were infected and developed PMWS and those that were infected but remained healthy.

Postweaning Multisystemic Wasting Syndrome (PMWS) in the Philippines: Porcine Circovirus Type 2 (PCV2) Detection and Characterization

2004 ◽  
Vol 66 (5) ◽  
pp. 533-537 ◽  
Author(s):  
Jaime MALDONADO ◽  
Joaquim SÉGALES ◽  
Maria CALSAMIGLIA ◽  
David LLOPART ◽  
Marina SIBILA ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Postweaning Multisystemic Wasting Syndrome ◽  
The Philippines ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Wasting Syndrome

Postweaning Multisystemic Wasting Syndrome: Experimental Studies with Porcine Circovirus Type 2

2008 ◽  
pp. 305-307
Author(s):  
Seamus Kennedy ◽  
Brian Meehan ◽  
Francis McNeilly ◽  
John Ellis ◽  
Steven Krakowka ◽  
...  
Keyword(s):  
Experimental Studies ◽  
Porcine Circovirus Type ◽  
Postweaning Multisystemic Wasting Syndrome ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Wasting Syndrome

scholarly journals Identification of porcine circovirus type 2 (PCV2), type 3 (PCV3) and porcine parvovirus (PPV) in swine by multiplex PCR test

2021 ◽  
Vol 20 (03) ◽  
pp. 11-17
Author(s):  
Phat X. Dinh
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Serum Samples ◽  
Single Target ◽  
Ns1 Gene ◽  
Type 3

This study aimed to simultaneously detect three important viruses reported to be involved in the reproductive problems of sows. A multiplex PCR (mPCR) test was developed to provide rapid diagnosis of porcine circovirus type 2 and 3 (PCV2, PCV3) and to illustrate parvovirus (PPV) prevalence in sow herds. Three pairs of specific primers were designed to target PCV2 Cap gene, PCV3 Cap gene and PPV NS1 gene, with predicted mPCR products of 702 bp, 267 bp and 380 bp, respectively. The detection limit of mPCR was 100 copies/reaction per target gene. The mPCR was run against a panel of 94 swine serum samples whose infection status had been pre-determined by commercial real-time PCR kits. Sequencing of mPCR products performed with clinical serum samples accurately confirmed the results. Overall, the results indicated that the mPCR functioned accurately and specifically and matched 100% with the single-target real-time PCRs. The mPCR was developed successfully and can be used in routine diagnosis of PCV2, PCV3 and PPV.

Intrahepatic icterus in pigs: rare clinical sign in porcine circovirus type 2 systemic disease

2020 ◽  
Vol 8 (3) ◽  
pp. e001193
Author(s):  
Robert Graage ◽  
Helena Saura Martinez ◽  
Stefanie Klausmann ◽  
Jakub Kubacki ◽  
Dolf Kümmerlen
Keyword(s):  
Systemic Disease ◽  
In Situ Hybridisation ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Fluorescence In Situ Hybridisation ◽  
Tissue Samples ◽  
Bilirubin Metabolism ◽  
Next Generation Sequencing Ngs

Icterus is a yellow discolouration of tissue and blood plasma due to a disorder of the bilirubin metabolism. This case report describes two porcine circovirus type 2 (PCV2) outbreaks in a fattening farm (F1) and a breeding farm (F2). At F1, pigs had intradermal bleedings and wasting, and three pigs showed icterus. At F2, suckling and weaning pigs (WPs) had an icterus and were wasted. Three fattening pigs (F1), one suckling piglet and one WP (F2) were dissected. Additional investigations included histology, PCV2 immunohistochemistry (IHC), fluorescence in situ hybridisation (FISH) of tissue samples, PCR including sequencing of the PCV2 strains and next-generation sequencing (NGS) of liver samples. Three of the five dissected pigs showed a lymphohistiocytic, periportal hepatitis with disruption of the hepatic cord architecture. PCV2 was detected by IHC, FISH, PCR and NGS in several organs of the pigs. In both farms, sequencing revealed a PCV2b genotype.

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