Changes in the Growth Characteristics and the Content of Decursin and Decursinol angelate in Angelica gigas Nakai by Mineral and Hormonal Agent Treatment

Ionic liquids (ILs) have gained much attention as alternative solvents to volatile organic solvents due to their attractive properties. This study aimed to develop an efficient method for the selective separation of decursin (D) and decursinol angelate (DA) from Angelica gigas Nakai (A. gigas) using ILs and crystallization. The IL 1-butyl-3-methylimidazolium tetrafluoroborate ((BMIm)BF4) was the most efficient at extracting D and DA. Parameters including solid-to-liquid ratio, time, and temperature were optimized by response surface methodology (RSM). Under optimal extraction conditions (1 g/6.5 mL solid-to-liquid ratio, 60 °C temperature, and 120 min time), the extraction yields of D and DA were 43.32 mg/g (97.06%) and 17.87 mg/g (97.12%), respectively. Moreover, drowning out crystallization using deionized water (DW) as an anti-solvent offered an excellent ability to recover D and DA from the A. gigas–(BMIm)BF4 extraction solution. The rates of recovery and the total purity of D and DA were found to be greater than 97%. Therefore, a rapid and efficient method of combining ILs with crystallization was effectively achieved for the selective separation of D and DA. This approach is assumed to be beneficial in the pharmaceutical industry for the effective obtention of D- and DA-enriched products.

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Enhanced accumulation of decursin and decursinol angelate in root cultures and intact roots of Angelica gigas Nakai following elicitation

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-010-9688-6 ◽

2010 ◽

Vol 101 (3) ◽

pp. 295-302 ◽

Cited By ~ 37

Author(s):

Hong Soon Rhee ◽

Hwa-Young Cho ◽

Seok Young Son ◽

Sung-Yong H. Yoon ◽

Jong Moon Park

Keyword(s):

Root Cultures ◽

Angelica Gigas ◽

Angelica Gigas Nakai ◽

Decursinol Angelate ◽

Intact Roots

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Effect of Angelica gigas Nakai extract on hepatic damage in rats

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i5.22 ◽

2020 ◽

Vol 19 (5) ◽

pp. 1059-1064

Author(s):

Won Tae Kim ◽

Kang Min Kim ◽

Jae Seon Kang

Keyword(s):

Liver Enzymes ◽

Antioxidant Activities ◽

Scavenging Activity ◽

Liver Mass ◽

Angelica Gigas ◽

Angelica Gigas Nakai ◽

Hepatocellular Damage ◽

Decursinol Angelate ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

Purpose: To determine the antioxidant and hepatoprotective effects of decursin and decursinol angelate (D/DA) isolated from Angelica gigas Nakai (AGN).Methods: The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of D/DA was assessed in a rat model using blood tests, western blotting, and histopathological analyses to identify the pharmaceutical effects of D/DA on liver enzymes and liver morphology.Results: The DPPH scavenging activity of D/DA was 47.11 μg/mL. Administration of D/DA to carbon tetrachloride (CCl4)-treated rats led to a decrease (13.59 %) in the total liver mass of control rats. Decursin and decursinol angelate also lowered the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), but increased the concentrations of antioxidant enzymes in the liver, including catalase (CAT) and glutathione peroxidase (GPx). Histological examination revealed that D/DA also reduced hepatocellular damage in the rats.Conclusion: D/DA from AGN has significant anti-hepatotoxic and antioxidant activities, and thus, is a potential herbal drug for treating liver damage. Keywords: Decursin, Decursinol angelate, Antihepatotoxicity, Antioxidant, Angelica gigas Nakai

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