scholarly journals Effect of Bamboo Salt on Inhibition of Adhesion of Candida albicans to Denture Acrylic Resin: An in vitro Study

Author(s):  
Keerthika Natarajan ◽  
Murugesan Krishnan ◽  
Suganya Srinivasan ◽  
Gowtham Venkat ◽  
Muthukumar Balasubramaniam
Author(s):  
Prabha Shakya ◽  
Deshraj Jain ◽  
Tripty Rahangdale

ABSTRACT Purpose Denture-related stomatitis is probably the most common form of oral candidiasis and its reported prevalence varies widely ranging up to 65%. In this in vitro study, we evaluate the effect of delmopinol application on Candida albicans adherence on heat-cured acrylic resin, cold-cured acrylic resin and microwave-cured acrylic resin. Materials and methods A total of 40 specimens of each type of acrylic resin were made; 20 specimens of each type were contaminated before delmopinol treatment and 20 specimens were contaminated after delmopinol treatment. The each specimen in each tube was individually transferred to a spectrophotometer at 530 nm wavelength in order to measure the turbidity degree, through the transmittance. Aliquots of 10 μl of each tube was then collected and inoculated into agar Sabouraud plates containing 500 mmol/l of sucrose, which was incubated for 24 hours at 37oC, in order to check microbial growth. Two-way ANOVA analysis of variance test and posthoc Turkey's test were carried out to ascertain the level of significance (p < 0.001) of various observations. Results Mean of the turbidity degree of contamination after delmopinol application for heat-cured acrylic resin specimens (A1) 1440.80, colony forming units (CFU) (⨯106/ml), cold-cured acrylic resin specimens (B1) 833.30 CFU (⨯106/ml) and microwave-cured acrylic resin specimens (C1) 944.70 CFU (⨯106/ml) was significantly higher than the mean of the turbidity degree of contamination before delmopinol application for heatcured acrylic resin specimens (A2) 550.85 CFU (⨯106/ml), coldcured acrylic resin specimens (B2) 341.45 CFU (⨯106/ml) and microwave-cured acrylic resin specimens (C2) 451.50 CFU (⨯106/ml). Conclusion In case of contamination after delmopinol application, heat-cured acrylic resin showed maximum reduction in adherence of C. albicans in the study. How to cite this article Shakya P, Jain D, Rahangdale T. Evaluation and Comparison of Effect of Delmopinol Application on Adherence of Candida albicans on Denture Fitting Surface on Three Types of Acrylic Resin: An in vitro Study. Int J Prosthodont Restor Dent 2012;2(4):129-135.


2021 ◽  
Vol Volume 12 ◽  
pp. 465-474
Author(s):  
Shereen A Sayed ◽  
Ehsan AB Hassan ◽  
Muhamad R Abdel Hameed ◽  
Michael N Agban ◽  
Mostafa F Mohammed Saleh ◽  
...  

2013 ◽  
Vol 3 (2) ◽  
pp. 60
Author(s):  
Subramaniam Ramanarayanan ◽  
Sakeenabi Basha ◽  
Mahesh Hiregoudar ◽  
PrashantGoudar Manjunath ◽  
Simpy Mittal ◽  
...  

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 179 ◽  
Author(s):  
Pier Carmine Passarelli ◽  
Marta De Leonardis ◽  
Giovan Battista Piccirillo ◽  
Viviana Desantis ◽  
Raffaele Papa ◽  
...  

Background: Peri-implantitis is an inflammatory disease with an increasing diffusion rate which can affect the long-term survival of a prosthetic rehabilitation. The present study focused on the decontaminating efficacy of chlorhexidine and air polishing system with sodium bicarbonate powder against Candida albicans, a microorganism which seems to have a superinfecting opportunistic role in the pathology. The aim of the authors was to investigate and compare the effectiveness of these treatments, commonly used in clinical practice. Methods: An in vitro study was conducted to analyze the effects of two widely used therapeutic aids for the disinfection of affected titanium implants: chlorhexidine (CHX) and air polishing with sodium bicarbonate powder (P). A qualitative and quantitative comparative analysis of the residual biofilm was carried out using a colorimetric assay (XTT) and scanning electron microscopy (SEM) observation. The experiment was conducted both on machined titanium surfaces and on rough sandblasted ones with the aim of bringing out differences in the therapeutic outcomes concerning the superficial texture of the implant. The null hypothesis was that no difference could be detected between the samples, regarding both the treatments performed and the nano-structural features of titanium. Results: The best results (on both types of implant surfaces) were obtained when combining the use of chlorhexidine and air polishing (C + P). A linear decrease in the optical density (OD) values recorded at three different time points (30 s, 1 min, 5 min) was also observed passing from the first to the last one. When observed under scanning electron microscope rough surfaces showed an extensive and highly structured biofilm, more complex if compared to the one encountered when analyzing machined implants. Conclusions: the present pilot study showed that rough surfaces can promote fungal adhesion and eventually hinder the outcome of a decontaminating treatment. For this purpose, the physio-chemical technique is always more efficient if compared to a single-technique approach regardless of the surface characteristics.


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