In Vitro Cultivation of the Exoerythrocytic Stage of Plasmodium Berghei in a Hepatoma Cell Line

SUMMARYThe causal prophylactic activity of the novel hydroxynaphthoquinone, S66C80, was assessed against the exo-erythrocytic (EE) stages of Plasmodium berghei cultured in the human hepatoma cell line, HepG2. 566C80 was found to be highly active as an inhibitor of EE development and was more active than the established causal prophylactic pyrimethamine. A 566C80 concentration of 1·85 × 10−9 M, added 3 h after sporozoite invasion, reduced the numbers of EE forms visible at 48 h by 50%, while the equivalent concentration of pyrimethamine was 1·95 × 10−8 M.

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Inhibitory effect of parvovirus H-1 on the formation of colonies of human hepatoma cell line in vitro and its tumors in nude mice

Cell Research ◽

10.1038/cr.1994.5 ◽

1994 ◽

Vol 4 (1) ◽

pp. 47-56 ◽

Cited By ~ 2

Author(s):

Shangjun Yan ◽

Chengwu Ma ◽

Xianhua Chen ◽

Shanhong Wan ◽

Zuyu Luo

Keyword(s):

Cell Line ◽

Nude Mice ◽

Hepatoma Cell ◽

Inhibitory Effect ◽

Hepatoma Cell Line ◽

Human Hepatoma ◽

Human Hepatoma Cell ◽

Human Hepatoma Cell Line

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Production of hepatitis B virus in vitro by transient expression of cloned HBV DNA in a hepatoma cell line.

The EMBO Journal ◽

10.1002/j.1460-2075.1987.tb04807.x ◽

1987 ◽

Vol 6 (3) ◽

pp. 675-680 ◽

Cited By ~ 112

Author(s):

C.M. Chang ◽

K.S. Jeng ◽

C.P. Hu ◽

S.J. Lo ◽

T.S. Su ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Cell Line ◽

Transient Expression ◽

Hepatoma Cell ◽

Hbv Dna ◽

Hepatoma Cell Line ◽

B Virus

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In Vitro Cytotoxicity of Oleanolic/Ursolic Acids-Loaded in PLGA Nanoparticles in Different Cell Lines

Pharmaceutics ◽

10.3390/pharmaceutics11080362 ◽

2019 ◽

Vol 11 (8) ◽

pp. 362 ◽

Cited By ~ 20

Author(s):

Amélia M. Silva ◽

Helen L. Alvarado ◽

Guadalupe Abrego ◽

Carlos Martins-Gomes ◽

Maria L. Garduño-Ramirez ◽

...

Keyword(s):

Cell Viability ◽

Cell Line ◽

Cell Lines ◽

Specific Activity ◽

Polymeric Nanoparticles ◽

Hepatoma Cell ◽

Plga Nanoparticles ◽

Hepatoma Cell Line ◽

Human Hepatoma Cell

Oleanolic (OA) and ursolic (UA) acids are recognized triterpenoids with anti-cancer properties, showing cell-specific activity that can be enhanced when loaded into polymeric nanoparticles. The cytotoxic activity of OA and UA was assessed by Alamar Blue assay in three different cell lines, i.e., HepG2 (Human hepatoma cell line), Caco-2 (Human epithelial colorectal adenocarcinoma cell line) and Y-79 (Human retinoblastoma cell line). The natural and synthetic mixtures of these compounds were tested as free and loaded in polymeric nanoparticles in a concentration range from 2 to 32 µmol/L. The highest tested concentrations of the free triterpene mixtures produced statistically significant cell viability reduction in HepG2 and Caco-2 cells, compared to the control (untreated cells). When loaded in the developed PLGA nanoparticles, no differences were recorded for the tested concentrations in the same cell lines. However, in the Y-79 cell line, a decrease on cell viability was observed when testing the lowest concentration of both free triterpene mixtures, and after their loading into PLGA nanoparticles.

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Electron Microscopy of Plasmodium Vivax Exoerythrocytic Schizonts Grown In Vitro in a Hepatoma Cell Line

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.1985.34.1017 ◽

1985 ◽

Vol 34 (6) ◽

pp. 1017-1021 ◽

Cited By ~ 10

Author(s):

Shigehiko Uni ◽

Masamichi Aikawa ◽

William E. Collins ◽

Michael R. Hollingdale ◽

Carlos C. Campbell

Keyword(s):

Electron Microscopy ◽

Plasmodium Vivax ◽

Cell Line ◽

Hepatoma Cell ◽

Hepatoma Cell Line

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Cell Clones Selected from the Huh7 Human Hepatoma Cell Line Support Efficient Replication of a Subgenomic GB Virus B Replicon

Journal of Virology ◽

10.1128/jvi.76.15.7736-7746.2002 ◽

2002 ◽

Vol 76 (15) ◽

pp. 7736-7746 ◽

Cited By ~ 20

Author(s):

Amedeo De Tomassi ◽

Maura Pizzuti ◽

Rita Graziani ◽

Andrea Sbardellati ◽

Sergio Altamura ◽

...

Keyword(s):

Host Cell ◽

Cell Line ◽

Hepatoma Cell ◽

Hepatoma Cell Line ◽

Human Hepatoma ◽

Human Hepatoma Cell ◽

Subgenomic Rna ◽

Human Hepatoma Cell Line ◽

Huh7 Cells

ABSTRACT Tamarins (Saguinus species) infected by GB virus B (GBV-B) have recently been proposed as an acceptable surrogate model for hepatitis C virus (HCV) infection. The availability of infectious genomic molecular clones of both viruses will permit chimeric constructs to be tested for viability in animals. Studies in cells with parental and chimeric constructs would also be very useful for both basic research and drug discovery. For this purpose, a convenient host cell type supporting replication of in vitro-transcribed GBV-B RNA should be identified. We constructed a GBV-B subgenomic selectable replicon based on the sequence of a genomic molecular clone proved to sustain infection in tamarins. The corresponding in vitro-transcribed RNA was used to transfect the Huh7 human hepatoma cell line, and intracellular replication of transfected RNA was shown to occur, even though in a small percentage of transfected cells, giving rise to antibiotic-resistant clones. Sequence analysis of GBV-B RNA from some of those clones showed no adaptive mutations with respect to the input sequence, whereas the host cells sustained higher GBV-B RNA replication than the original Huh7 cells. The enhancement of replication depending on host cell was shown to be a feature common to the majority of clones selected. The replication of GBV-B subgenomic RNA was susceptible to inhibition by known inhibitors of HCV to a level similar to that of HCV subgenomic RNA.

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Development of a Hybrid Polymer-Based Microfluidic Platform for Culturing Hepatocytes towards Liver-on-a-Chip Applications

Polymers ◽

10.3390/polym13193215 ◽

2021 ◽

Vol 13 (19) ◽

pp. 3215

Author(s):

Gulsim Kulsharova ◽

Akbota Kurmangaliyeva ◽

Elvira Darbayeva ◽

Luis Rojas-Solórzano ◽

Galiya Toxeitova

Keyword(s):

Cell Line ◽

Volume Fraction ◽

Hepatoma Cell ◽

Perfusion Culture ◽

Bottom Layer ◽

Hepatoma Cell Line ◽

Dynamics Modeling ◽

Microfluidic Platform ◽

Huh7 Cells

The drug development process can greatly benefit from liver-on-a-chip platforms aiming to recapitulate the physiology, mechanisms, and functionalities of liver cells in an in vitro environment. The liver is the most important organ in drug metabolism investigation. Here, we report the development of a hybrid cyclic olefin copolymer (COC) and polydimethylsiloxane (PDMS) microfluidic (HCP) platform to culture a Huh7 hepatoma cell line in dynamic conditions towards the development of a liver-on-a-chip system. The microfluidic platform is comprised of a COC bottom layer with a microchannel and PDMS-based flat top layer sandwiched together. The HCP device was applied for culturing Huh7 cells grown on a collagen-coated microchannel. A computational fluid dynamics modeling study was conducted for the HCP device design revealing the presence of air volume fraction in the chamber and methods for optimizing experimental handling of the device. The functionality and metabolic activity of perfusion culture were assessed by the secretion rates of albumin, urea, and cell viability visualization. The HCP device hepatic culture remained functional and intact for 24 h, as assessed by resulting levels of biomarkers similar to published studies on other in vitro and 2D cell models. The present results provide a proof-of-concept demonstration of the hybrid COC–PDMS microfluidic chip for successfully culturing a Huh7 hepatoma cell line, thus paving the path towards developing a liver-on-a-chip platform.

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