Abstract
Carbon Nanotubes (CNTs) are cylindrical nanostructures, and have unique combination of properties including flexibility, electrical conductivity, and biocompatibility. We focused on CNTs fabricated with the carbon nanotube yarns (cYarn®) as a possible substrate that could promote peripheral nerve regeneration with these properties. We bridged a 15mm rat sciatic nerve defect with five different density of cYarn®. Eight weeks after the surgery, the density of regenerated axons crossing the CNTs, electromyographical findings, and muscle weight ratio of the lower leg showed recovery of the motor function by the interfacing with cYarn®. Our results indicated that a 2% CNT density tended to be most effective for nerve regeneration as measured by both histological axonal regeneration and motor function. We confirmed that CNT yarn, used as a scaffold bridging nerve gaps, promotes peripheral nerve regeneration. Our results support the future clinical application of CNTs for bridging nerve gaps as an off-the-shelf material.