defocused imaging
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The Analyst ◽  
2020 ◽  
Vol 145 (18) ◽  
pp. 6038-6044 ◽  
Author(s):  
Jaeran Lee ◽  
Geun Wan Kim ◽  
Ji Won Ha

Total internal reflection scattering (TIRS) microscopy provided higher S/N ratio and more accurate angular information of gold nanorods.


2019 ◽  
Vol 97 (6) ◽  
pp. 422-429
Author(s):  
Hamid Soleimaninejad ◽  
Kenneth P. Ghiggino ◽  
Trevor A. Smith ◽  
Matthew F. Paige

UV-illumination of phase-separated surfactant films prepared from mixtures of photopolymerizable 10,12-pentacosadiynoic acid and perfluorotetradecanoic acid results in the formation of fluorescent polydiacetylene fibers and aggregates. In this work, the orientation of polymer strands that comprise the resulting photopolymer structures has been probed using fluorescence anisotropy imaging in combination with defocused single-molecule fluorescence imaging. Imaging experiments indicate the presence of significant fiber-to-fiber heterogeneity, as well as anisotropy within each fiber (or aggregate), with both of these properties changing as a function of film preparation conditions. This anisotropy can be attributed to various alignments of the constituent polymer strands that comprise the larger fibers and aggregates. Intriguingly, when using defocused imaging, fiber images consisted of a series of discrete “doughnut” fluorescence emission patterns, which exhibited intermittent on–off blinking behavior; both of these properties are characteristic of individual emission transition dipoles (single molecules). Further, all of the individual emission transition dipoles had a uniform orientation with respect to the axis of the fiber, indicating a common orientation of discrete emitters in the larger polymer fiber. The implications of these results for future studies of the electronic properties of conjugated polymers in larger macroscopic systems are noted.


Author(s):  
Lijun Wu ◽  
Fanwei Zhang ◽  
Qiang Li ◽  
Wenye Rao ◽  
Hongjin Hu ◽  
...  
Keyword(s):  

2017 ◽  
Vol 35 (12) ◽  
pp. 1488-1496 ◽  
Author(s):  
De-sheng Li ◽  
Jing-fa Yang ◽  
Jiang Zhao

ACS Photonics ◽  
2017 ◽  
Vol 4 (8) ◽  
pp. 2003-2010 ◽  
Author(s):  
Tao Li ◽  
Fanwei Zhang ◽  
Qiang Li ◽  
Wenye Rao ◽  
Pei Xu ◽  
...  

2017 ◽  
Vol 139 (21) ◽  
pp. 7156-7159 ◽  
Author(s):  
Bartosz Krajnik ◽  
Jiawen Chen ◽  
Matthew A. Watson ◽  
Scott L. Cockroft ◽  
Ben L. Feringa ◽  
...  

2016 ◽  
Vol 14 (3) ◽  
pp. 031201-31205 ◽  
Author(s):  
Wu Zhou Wu Zhou ◽  
Na Jin Na Jin ◽  
Minhua Jia Minhua Jia ◽  
Huinan Yang Huinan Yang ◽  
and Xiaoshu Cai and Xiaoshu Cai

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