A large, square-shaped, DNA origami nanopore with sealing function on a giant vesicle membrane

DNA origami nanopore with large size of a 10 nm square, equipping a tunable lid, enables size-selective molecular transportation through the lipid membrane of giant vesicle.

Sealable Large Pore by DNA Origami on Lipid Membrane

Here, we report on the design and function of a membrane nanopore through a DNA origami square tube with a cross-section of 100 nm2 . When the nanopore is added onto the giant vesicle membrane, the permeation of hydrophilic fluorescent molecules was observed. It can be sealed by the existence of the four specific single strand DNAs. A controllable artificial nanopore should help to communicate the vesicle components with their environment

Sealable Large Pore by DNA Origami on Lipid Membrane

Here, we report on the design and function of a membrane nanopore through a DNA origami square tube with a cross-section of 100 nm2 . When the nanopore is added onto the giant vesicle membrane, the permeation of hydrophilic fluorescent molecules was observed. It can be sealed by the existence of the four specific single strand DNAs. A controllable artificial nanopore should help to communicate the vesicle components with their environment

Dynamic monitoring of a bi-enzymatic reaction at a single biomimetic giant vesicle

Giant unilamellar vesicles were used as individual biomimetic micro-reactors wherein a model bi-enzymatic reaction involving a glucose oxidase (GOx) and horseradish peroxidase (HRP) was monitored by confocal microscopy.

Engineering bio-mimicking functional vesicles with multiple compartments for quantifying molecular transport

Giant vesicle functional models mimicking a bacterial membrane under physiological conditions are constructed.