PCR Primer Spesifik Berdasarkan Gen Cytochrome b untuk Deteksi Garangan (Herpestes javanicus) secara Molekuler

Garangan (Herpestes javanicus) merupakan salah satu spesies meso-carnivora yang berperan penting dalam ekosistem sebagai pemangsa. Kehadirannya dianggap mampu mengendalikan populasi spesies mangsa, termasuk beberapa spesies hama. Namun anggapan ini dianggap hanya anekdot karena kekurangan data ekologis pendukungnya sebagai dampak dari kesulitan pengamatan visual atas spesies ini. Penelitian ini bertujuan untuk merancang PCR primer untuk deteksi Garangan secara molekuler dari material organik yang ditinggalkan, misalnya kotoran. Perancangan PCR primer dimulai dengan memilih penanda spesifik Garangan yang melibatkan 51 sekuen DNA gen Cytochrome-b dari 19 spesies karnivora Jawa, menentukan primer forward dan reverse, dan menguji in-silico dan in vitro dari primer yang berhasil dirancang. Proses tersebut dilakukan dengan bantuan software MEGA 5 dan SP-Designer, dan memanfaatkan basis data genetik dari GenBank (NCBI). Uji in silico dan in vitro menunjukkan bahwa sekuen primer forward 5’- CAAATCACACCCACTCATTAAAATC-3’ dan reverse 5’-TGTGGGTTACTGATGAAAAGG-3’ akan mampu mendeteksi Garangan secara molekuler dari material organik spesies ini. Penelitian ini berkontribusi dalam pengumpulan data dasar kehadiran Garangan untuk mengumpulkan informasi lanjut tentang okupansi, distribusi spesies, dan pakannya. Informasi ini merupakan fondasi untuk memahami peran spesies ini dalam ekosistem. Specific PCR Primers Based on Cytochrome-b Gene for Molecular Detection of JavanMongoose (Herpestes javanicus)AbstractJavan Mongoose is a meso-carnivore species that have an important role in ecosystem as a predator. It is believed that its occurrence controls prey populations including some pest species. However, the belief is an anecdote because of lacking supportive data. Difficulty in visually observing the species is a major problem preventing data collection. This study aims to design PCR primer for detecting the Mongoose molecularly from organic material remaining, such as feces. The design starts with selecting specific marker from 51 DNA sequences of cytochrome-b. The DNA sequences were of 19 Javan carnivore collected from GenBank (NCBI). PCR primers were designed and tested using both in-silico and in-vitro techniques. The sequence collection and selection, and primer design process employed MEGA 5 and SP-Designer software. We successfully designed PCR primers: forward 5’- CAAATCACACCCACTCATTAAAATC-3’ dan reverse 5’-TGTGGGTTACTGATGAAAAGG-3’. The primer is capable of detecting Javan Mongoose from remaining organic material. The study contributed to basic data collection of Javan Mongoose for advanced studies such as occupancy modeling, species distribution modeling and diet analysis. This information is fundamental for understanding the role of the Javan Mongoose in an ecosystem.

Comparative Histological Study of the Stomach in Two Species of Iraqi Vertebrates (Magpie Pica pica L. and Small Asian Mongoose Herpestes javanicus E.)

A histological study showed the wall of the stomach in Pica pica and Herpestes javanicus consists of four layers: mucosa, submucosa, muscularis externa and serosa. Also, the present study showed many differences in the histological structures of the stomach for each in both types. The stomach of P. pica consists of two portions: the proventiculus and gizzard, while the stomach of H. javanicus consists of three portions: cardiac, fundic and pyloric regions. The mucosa layer formed short gastric folds, named plicae. In the proventiculus of P. pica, sulcus is found between each two plicae, but the folds called gastric pits in the gizzard, which are full with koilin. Lamina properia in both types contained gastric glands (straight simple tubular glands) named superficial glands, as well as another gastric gland found in the submucosa layer of the proventiculus in P. pica only named deep gastric glands. The gastric gland in the stomach of H. javanicus contained: mucous neck cells and parietal cells positive to AB/PAS stains in cardiac portion, as well as chief cells in fundic portion, but pyloric portion had just mucous neck cells. Muscularis externa in both types formed two muscle layers: inner and outer layer.

Comparative Histological Study of the Stomach in Two Species of Iraqi Vertebrates (Magpie Pica pica L. and Small Asian Mongoose Herpestes javanicus E.)

A histological study showed the wall of the stomach in Pica pica and Herpestes javanicus consists of four layers: mucosa, submucosa, muscularis externa and serosa. Also, the present study showed many differences in the histological structures of the stomach for each in both types. The stomach of P. pica consists of two portions: the proventiculus and gizzard, while the stomach of H. javanicus consists of three portions: cardiac, fundic and pyloric regions. The mucosa layer formed short gastric folds, named plicae. In the proventiculus of P. pica, sulcus is found between each two plicae, but the folds called gastric pits in the gizzard, which are full with koilin. Lamina properia in both types contained gastric glands (straight simple tubular glands) named superficial glands, as well as another gastric gland found in the submucosa layer of the proventiculus in P. pica only named deep gastric glands. The gastric gland in the stomach of H. javanicus contained: mucous neck cells and parietal cells positive to AB/PAS stains in cardiac portion, as well as chief cells in fundic portion, but pyloric portion had just mucous neck cells. Muscularis externa in both types formed two muscle layers: inner and outer layer.