One of the relevant tasks of current morphology is the investigation of tissue regenerative potential and research for new medications that improve recovery process efficiency. Nowadays, clinical specialists focus on medications that are based on natural compounds. However, such medication influence on the processes that occur within the injured tissues is still not identified. The purpose of this study was to investigate skeletal muscle tissue posttraumatic regeneration response to humic peloid medication, based on humic acids modified by Zinc ions. Humic acid extraction was carried out by means of patent procedure. The study included laboratory Wistar rats with hyperextension of front femur muscle. The preparations were studied by means of light and electronic microscopy and autoradiography. Histologic preparations evaluation showed that under peloid medication exposure the apolexis processes within muscle fibers rupture are inhibited, interstitial edema becomes restricted by the injured area, vessel growth into the area of damaged capillaries is stimulated, macrophages migrate and the area and duration of posttraumatic inflammation decrease. Additionally, peloid medication intake shortens the length of skeletal muscle tissue reparative histogenesis stages: myosatellitocytes are activated earlier than in the control group, myoblasts and myosymplasts are detected, the separation of nuclear sarcoplasmatic areas from partly injured muscle fibers is stimulated, myotubules appear 3 – 5 days earlier than in control group. Overall, muscle tissue regeneration efficiency increases by 21%. Obtained results allow us to conclude that peloid medication based on humic acids modified by Zinc ions positively influence the stimulation of the regeneration process. This will lead to further investigation of humic substances: fulvic, hymatomelanic, humin and humic acids of peloids as medications and their implementation in clinical practice.
SKELETAL MUSCLE POSTTRAUMATIC REGENERATION RESPONSE TO HUMIC PELOID MEDICATION
