STUDY OF THE SHELL MATTER OF MODERN AND PLEISTOCENE MOLLUSCS OF THE GENUS DIDACNA

One of the ways to study the reactions of marine invertebrates to the external effects of changes in temperature and salinity is the biogeochemical analysis of skeletal parts, which are consistently built up during ontogenesis and record a variety of information about these changes. The most studied shells of mollusks, sea urchin shells and skeletal parts of corals. Information about the chemical composition of modern and fossil mollusk shells is widely used in solving geological and biological problems, including determining the temperature and salinity of ancient marine basins, studying the diagenesis of carbonate sediments, and the biochemical evolution of invertebrates. X-ray diffraction analysis of the shell matter of didacnae belonging to the Cardiidae showed an aragonite composition. The quantitative determination of elements in mollusk shells by microprobe analysis of spot scanning and spectrometric method is carried out. Samples were taken in successive layers of shell growth within the annual ring, and the seasonal dynamics of strontium changes were detected. For Didacna, strontium is the main element-indicator of seasonal and ontogenetic growth, is included in the crystal lattice of aragonite and forms strong compounds in the process of shell formation during the life of these bivalves. The variability of seasonal, ontogenetic, and taxonomic differences in a number of indicator elements in living and Pleistocene bivalves of the genus Didacna was studied.

A Novel Polishing Paste (Mollusk Shells) for Poly (Methylmethacrylate)

Objective. The aim of this study was to evaluate the effectiveness of a mollusk shells polishing paste (Donax obesulus) on the surface roughness of acrylic resin poly (methylmethacrylate) (PMMA). Methods. This study was an in vitro experimental design. A sample size of 72 was divided into 4 groups of n = 18 each. PMMA specimens were prepared and polished with the evaluated pastes using mollusk shells (experimental paste) and pumice stone. Surface roughness (μm) was measured using a profilometer after polishing the PMMA samples. The paired Wilcoxon test was used to evaluate the roughness values at 24 and 48 hours. Then, the Mann–Whitney U test was used to identify the differences between the effects of the two groups evaluated with a significance level of α = 0.05. Results. The roughness difference between the pastes under study was compared, and mean values of 0.50 ± 0.07 μm (mollusk shell paste group) and 0.45 ± 0.12 μm (pumice group) were obtained. No statistically significant differences were found between the experimental paste and pumice stone paste p = 0.309 . The specimens polished with pumice stone paste showed higher roughness values, while those polished with the experimental paste exhibited the lowest values. Conclusion. In summary, mollusk shells polishing paste had a decrease in roughness values compared to pumice, although these differences were not statistically significant.

Hyperspectral interference tomography of nacre

Structural characterization of biologically formed materials is essential for understanding biological phenomena and their enviro-nment, and for generating new bio-inspired engineering concepts. For example, nacre—the inner lining of some mollusk shells—encodes local environmental conditions throughout its formation and has exceptional strength due to its nanoscale brick-and-mortar structure. This layered structure, comprising alternating transparent aragonite (CaCO3) tablets and thinner organic polymer layers, also results in stunning interference colors. Existing methods of structural characterization of nacre rely on some form of cross-sectional analysis, such as scanning or transmission electron microscopy or polarization-dependent imaging contrast (PIC) mapping. However, these techniques are destructive and too time- and resource-intensive to analyze large sample areas. Here, we present an all-optical, rapid, and nondestructive imaging technique—hyperspectral interference tomography (HIT)—to spatially map the structural parameters of nacre and other disordered layered materials. We combined hyperspectral imaging with optical-interference modeling to infer the mean tablet thickness and its disorder in nacre across entire mollusk shells from red and rainbow abalone (Haliotis rufescens and Haliotis iris) at various stages of development. We observed that in red abalone, unexpectedly, nacre tablet thickness decreases with age of the mollusk, despite roughly similar appearance of nacre at all ages and positions in the shell. Our rapid, inexpensive, and nondestructive method can be readily applied to in-field studies.

The Debris Distribution Model for Removal Planning of an Urbanized Estuarine Complex

We checked the spatial pattern of debris stranding at Praia de Santos (Brazil) in the first hour after the peak of high and low tide in summer and winter. We recorded and counted 20 types of debris (natural and anthropogenic) in 6 transects arranged in 3 zones along the beach (deposition, transport, and erosion). Canonical Correspondence Analysis (CCA) showed that buoyancy was an essential property in distinguishing debris stranding behaviours. The erosion zone, closer to the estuary showed that many mollusk shells, ropes and rocks, while plastics, styrofoam and fiber nylon were in the deposition zone. Throughout the estuary, we inspected the beach, identifying sections of origin and accumulation associated with the circulation system and currents. We propose the removal and recycling of debris from accumulation sites near highways and urban systems.

Temperatures of Late Cretaceous (Campanian) methane-derived authigenic carbonates from the Western Interior Seaway, South Dakota, USA, using clumped isotopes

Methane seep deposits, comprising large, carbonate-rich mounds formed from hydrocarbon seepage, were widely distributed in the Late Cretaceous Western Interior Seaway (WIS) of North America. Well-preserved, methane-derived authigenic carbonates (MDACs) from these deposits have been shown to retain petrological, paleontological, and geochemical imprints of their ancient depositional setting, all of which are important for understanding the dynamics and evolution of the shallow, epeiric WIS. To better characterize the environmental conditions of WIS seeps, we applied clumped isotope paleothermometry to magnesium calcite MDAC samples from five seep localities in the upper Campanian Pierre Shale, South Dakota, USA. We measured 21 subsamples, including 18 micritic carbonates and demonstrated apparent clumped isotope equilibrium between MDACs and WIS bottom waters. Extreme 13C depletion in most samples (δ13C ranging to −45.44‰) indicates they were precipitated with oxidized methane as a major source of dissolved inorganic carbon, which itself implies a close association with ancient methanotrophic metabolism. The average clumped isotope paleotemperature from the micritic carbonates is 23 ± 7 °C (1σ standard deviation), which agrees with bottom water paleotemperatures inferred from δ18O measurements of MDACs and well-preserved mollusk shells at similar localities in the WIS. The calculated average δ18Ow value for these samples is −0.5 ± 1.7‰ (1σ SD), which is indistinguishable from previously reported calculation on Campanian seawater δ18Ow from fossil mollusk shells, but elevated above younger fossils collected from other locations in the WIS. Our conclusions are inconsistent with previously hypothesized disequilibrium for WIS MDAC clumped isotope and therefore we propose that fossil MDAC deposits may be used as paleotemperature archives.

Theoretical investigation of mollusk shells: Energy landscape exploration of CaCo3 polymorphs and element substitution: A short review

Due to the remarkable properties achieved under ambient conditions and with quite limited components, mollusk shells are very appealing natural bio-composites used as inspiration for new advanced materials. Calcium carbonate which is among the most widespread biominerals is used by mollusks as a building material that constitutes 95-99% of their shells. Within the investigation of calcium carbonate polymorphs present in the shells, diverse theoretical and experimental studies were performed, however, further research of these crystalline forms is required. There are very little researches on the energy landscapes of biogenic calcium carbonate which can provide us information about the free energies of already known as well as newly discovered plausible structures. To investigate the structural, mechanical, elastic, or vibrational properties and to predict new possible structures of biogenic calcium carbonate, different calculation methods could be employed. Some of these studies are presented and discussed in this paper.