Bacterial Source Tracking of Nonpoint Source Pollution Using GIS and DNA Fingerprinting Technologies

This study is only the second to use DNA fingerprinting technology in Maryland to identify fecal coliform sources in order to guide the implementation of water pollution control practices in a watershed with bacterial impairment. By combining the use of digital air photos and GPS with GIS, fieldwork is planned and conducted more efficiently because sample sites can be selected that accurately represent the physical environment of the study area. We can also return to the field and find our sample sites or locate new ones, even in the remotest part of the study area. It is also possible to more accurately map the data directly in the context of its physical environment, greatly increasing the quality of analysis. The integration of DNA fingerprinting techniques with GIS shows great promise for extending our capabilities to identify the controls on water quality and point sources of waterborne health hazards.