scholarly journals Toxicity Study of 1-(2, 5-Dihidroxyphenil)-3-Pyridine-2-Il-Propenone In Adult Female Mice

2021 ◽  
Author(s):  
Ika Puspitasari ◽  
Ratna Asmah Susidarti
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Toxicity Test ◽  
Biochemical Parameters ◽  
Chronic Toxicity ◽  
Histological Analysis ◽  
Lymphocytic Infiltration ◽  
Experimental Animals ◽  
Female Mice ◽  
Chronic Toxicity Test

This research aimed to evaluate the toxicity of 1-(2, 5-dihidroxyphenil)-3-pyridine-2-il-propenone (DPP) after 24 hours and 90-day administration in female mice. Acute toxicity test was performed using the OECD 423 method, and DPP was administered once a day at doses of 300, 2000, and 5000 mg/kg body weight (BW). Toxic symptoms were observed after 24 hours of administration, and this continued until the 14th day. The experimental animals were dissected and examined for histological organs on the 15th day. The sub-chronic toxicity test was performed using the OECD 408 method, and DPP at 14, 28, and 56 mg/kg/day was administered for 90 days. Toxic symptoms were observed every day, and the amount of food and water intakes were also measured. Furthermore, statistical analysis was performed, and changes in body weight as well as routine blood checks and biochemistry were observed. At the end of the study, experimental animals were killed and the vital organs' weights were examined before their histological analysis. The results showed that DPP at 300-5000 mg/kg/day and 14-56 mg/kg/day for 90 days did not show any toxic symptom respectively. In the sub-chronic toxicity test, no change was observed in blood and urine biochemical parameters (p≥0.05). However, lymphocytic infiltration in the liver and congested vessel in the kidney occurred after administration at 56 mg/kg / day. The results showed that the acute toxicity of DPP is at category 5 according to Globally Harmonized Classification System and sub-chronic toxicity is at a dose below 56 mg/kg/day.

Acute and sub-chronic oral toxicities of DA.AMLODEPON HVD hard capsule in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 58-67
Author(s):  
Pham Thi Van Anh ◽  
Nguyen Van Dam ◽  
Nguyen Van Dat ◽  
Pham Thanh Ky ◽  
Nguyen Trong Thong ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Chronic Toxicity ◽  
The Body ◽  
Toxicity Study ◽  
World Health ◽  
Histopathological Analysis ◽  
Experimental Animals ◽  
Health Organization ◽  
General Conditions

Assessment of toxicities of DA.AMLODEPON HVD hard capsule on experimental animals. The acute toxicity of DA.AMLODEPON HVD was assessed on Swiss mice according to World Health Organization Guidance, and LD50 determination according to the method of Litchfield – Wilcoxon. The sub-chronic toxicity study of DA.AMLODEPON HVD at two doses (0.42 g/kg/day and 1.26g/kg/day) was conducted in rats for four consecutive weeks. After administration, general conditions and the body weight of rats were evaluated. Blood samples were collected for analyzing serum parameters before treatment (T0), second week (T1), and fourth week (T2). Histopathological analysis of livers and kidneys was observed at the end of the experiment. The results revealed that mice were taken up to a maximum dose of 39.15 g/kg with no symptoms of acute toxicity, LD50 of DA.AMLODEPON HVD has not been determined. The sub-chronic toxicity study at two doses did not change the body weight of rats, general conditions. The parameters for structures and functions of livers and kidneys and microscopic of the livers and kidneys are in a normal range during the study period.

scholarly journals Acute and subchronic oral toxicities of “Da day An Chau” tablets in experimental animals

2021 ◽  
Vol 141 (5) ◽  
pp. 1-9
Author(s):  
Tran Thanh Tung ◽  
Dao Viet Hoang ◽  
Pham Thi Van Anh ◽  
Dang Thi Thu Hien
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
World Health Organization ◽  
Biochemical Parameters ◽  
Maximum Dose ◽  
Toxicity Study ◽  
World Health ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Health Organization

In this study, the toxicities of “Da day An Chau” tablets (DDAC) were assessed on experimental animals. To evaluate the acute toxicity on Swiss mice according to World Health Organization Guidance and to determine LD50 refer to the method of Litchfield – Wilcoxon. The subchronic toxicity study of DDAC at two doses (0.58 g/kg/day and 1.74 g/kg/day) was conducted in rats for four consecutive weeks. Evaluation of general conditions and weight of rats during the study period. Rat’s blood was taken for hematological and biochemical evaluations. The livers and kidneys microscopes were evaluated at the end of the experiment. The result revealed that mice were taken up to a maximum dose of 25.71 g/kg with no symptoms of acute toxicity; LD50 of DDAC has not been determined. At two doses, the subchronic toxicity study did not change rats’ body weight, hematological, biochemical parameters, and microscopic of the livers and kidneys during the study period.

scholarly journals Safety Profile of Meswak Root Extract on Liver, Kidney, Sexual Hormones and Hematological Parameters of Rats

2012 ◽  
Vol 4 (1) ◽  
pp. 18-23
Author(s):  
Abeer Y. IBRAHIM ◽  
Souad E. El-GENGAIHI
Keyword(s):  
Acute Toxicity ◽  
Safety Profile ◽  
Toxicity Test ◽  
Biochemical Parameters ◽  
Chronic Toxicity ◽  
Female Rats ◽  
Root Extract ◽  
Acute Toxicity Test ◽  
Sexual Hormones ◽  
Delayed Toxicity

This study was conducted to investigate the safety profile of Salvadora persica (Salvadoraceae) aqueous alcoholic root extract by carrying out acute and sub-chronic toxicity assessment in order to find out any side effect of the traditionally using of these root sticks. Regarding to acute toxicity test, mice were administered the extract up to 5 g kg-1, intraperitoneally. Animals were then observed for behavioural changes; signs of toxicity, and mortality within 24 h. Surviving mice were monitored for 7 days for signs of delayed toxicity. In the sub-chronic toxicity test, rats were daily treated with the extract at a dose of 400 mg kg-1 intraperitoneally, for 30 days. At the end of the test period, hematological and biochemical parameters were determined in blood and serum samples with determination of vital organs weights. In the acute toxicity test, the extract was practically non-toxic showing no mortality and visible signs of delayed toxicity. The LD50, given intraperitoneally, was estimated to be 4 g kg-1. Administration of extract (at a dose of 400 mg Kg-1 b.wt.) to male and female rats for 30 days did not produce any significant (P < 0.05) effect on hematological and most biochemical parameters also vital organs weights. The root extract showed adverse effects on sexual hormones, by increasing estrogen secretion and reducing testosterone level in male rats. At the same time, the extract reduces progesterone level in female satellite group. Overall, Meswak aqueous extract is safe concerning liver and kidney functions and hematological assessments; however, it induces reversal effect on sexual hormones levels determined in sera.

Effects of atrazine and alachlor on self-purification processes in receiving streams

1996 ◽  
Vol 33 (6) ◽  
pp. 181-187 ◽  
Author(s):  
Jana Zagorc-Koncan
Keyword(s):  
Adverse Effect ◽  
Acute Toxicity ◽  
Toxicity Test ◽  
Chronic Toxicity ◽  
Acute Toxicity Test ◽  
Organic Substances ◽  
Inhibiting Effect ◽  
Scenedesmus Subspicatus ◽  
Net Assimilation ◽  
Chlorophyll A Content

In recent years many waterways in Slovenia have been subjected to an increased loading with pesticides due to intensification of agriculture. The most widely used herbicides are atrazine and alachlor and they were detected in some rivers and even in ground water. Therefore the effects of atrazine and alachlor on selfpurification processes were investigated. The basic selfpurification processes studied were biodegradation of organic substances and photosynthesis and growth of algae. The inhibiting effect of pesticides on the process of biodegradation of organic pollutants was evaluated by the use of laboratory river model and mathematical modelling. The harmful impacts of pesticides on aquatic autotrophic organisms were assessed by measurement of net assimilation inhibition (24-h acute toxicity test) as well as growth inhibition - chlorophyll- a content (72-h chronic toxicity test) of algae Scenedesmus subspicatus. The results obtained demonstrate that atrazine and alachlor in concentrations found in our rivers have practically no effect on biodegrading heterotrophic organisms, while their adverse effect on algae is quite considerable.

scholarly journals Chronic toxicity test with sea urchin Echinometra lucunter and Lytechinus variegatus (Echinodermata: Echinoidea), exposed to light-stick - flag paternoster used for longline surface fishing

2010 ◽  
Vol 58 (spe3) ◽  
pp. 71-75 ◽  
Author(s):  
Caio Cesar-Ribeiro ◽  
Maria Fernanda Palanch-Hans
Keyword(s):  
Toxicity Test ◽  
Chronic Toxicity ◽  
Sea Water ◽  
Sea Urchins ◽  
Preliminary Test ◽  
Lytechinus Variegatus ◽  
Confidence Limits ◽  
Potential Danger ◽  
Final Test ◽  
Chronic Toxicity Test

In this work, the chronic toxicity of a mixture of light-stick chemicals and water was tested. The light-stick is used in fishery activities to catch swordfish. The tubes were collected on the beaches of the Costa dos Coqueiros - BA, Brazil, in the period from 14th to 31st July 2007. The method used was a short chronic toxicity test where embryos of the sea urchins Echinometra lucunter and Lytechinus variegatus were exposed to a stock solution consisting of the supernatant formed from a mixture of sea water and the orange-colored light-stick chemical. After a preliminary test, concentrations defined were 0.002, 0.003, 0.01, 0.02, 0.1, 1.0% of stock solution. The final test ran for 36 hours for E. Lucunter and 24 hours for L. variegatus with 4 replicates for each concentration. The value of EC50 - 36h was 0.062% with confidence limits ranging from 0.042 to 0.079% and the EC50 - 24h was 0.011% with confidence limits ranging from 0.009 to 0.014%, i.e., the chemical mix present in the light-stick is potentially toxic. So, as these flags are commonly used for fishing there is potential danger in their disposal in the open ocean.

scholarly journals SUB-ACUTE TOXICITY OF A STEROIDAL GLYCOSIDE FROM THE FLOWERS OF ALANGIUM SALVIIFOLIUM WANG

2021 ◽  
Vol 12 (6) ◽  
pp. 35-39
Author(s):  
Adeeba Anjum ◽  
Ashik Mosaddik ◽  
Mir Imam Ibne Wahed ◽  
Md. Ekramul Haque
Keyword(s):  
Clinical Trials ◽  
Acute Toxicity ◽  
Biochemical Parameters ◽  
Chronic Toxicity ◽  
Preliminary Investigation ◽  
Control Group ◽  
Blood Samples ◽  
Steroidal Glycoside ◽  
Liver And Kidney ◽  
Hematological And Biochemical Parameters

The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl-(24)-ethylcholesta-5,22,25-triene, a steroidal glycoside isolated from the flowers of Alangium salviifolium Wang on Long Evan’s rat. After intra-peritoneal administration of the compound at a dose of 300 μg/rat/day for 14 consecutive days, no mortality or significant changes in body weight or behavior were observed. The blood samples of the rats were examined for hematological and biochemical parameters which were statistically insignificant when compared to that of the control group. All the vital organs showed normal histopathological architecture (heart, lungs, liver and kidney) in comparison to the control group. This preliminary investigation demonstrate that the compound is safe at dose of 300 μg/rat/day for 14 consecutive days. But acute, sub-chronic and chronic toxicity evaluations as well as clinical trials need to be done.

scholarly journals Przykłady wykorzystania testu OSTRACODTOXKIT F™ do oceny zanieczyszczenia pyłów drogowych metalami w aglomeracji wrocławskiej

2020 ◽  
Vol 29 (1) ◽  
pp. 27-36
Author(s):  
Magdalena Wróbel ◽  
Wioletta Rogula-Kozłowska ◽  
Justyna Rybak
Keyword(s):  
Growth Inhibition ◽  
Toxicity Test ◽  
Chronic Toxicity ◽  
Road Dust ◽  
Water Bodies ◽  
City Centre ◽  
Aquatic Life ◽  
Chronic Toxicity Test ◽  
Living Organisms ◽  
The Impact

Pollutants present in road dust deriving from traffic, such as PAHs and other organic compounds or heavy metals, are washed out with rain and get into the water bodies accumulating in sediments for many years and simultaneously posing a threat to aquatic life and significantly affecting water quality. To study the impact of these toxic compounds on the health of living organisms, routine tests of water and sediments chemistry are insufficient as studies based on living organisms are much more reliable. And therefore, in order to know the response of living organisms to road dust pollutants which enter the water bodies the chronic toxicity test OSTRACODTOXKIT F™ was used. This test is based on the observation of development of Heterocypris incongruens that normally lives in sediments. Ostracod, H. incongruens is very sensitive to heavy metal contamination, thus it is a very good tool to study toxicity of road dust washed out with rain into the water bodies. The research was conducted in the Wrocław agglomeration (in the city centre and suburbs) at sites differing in the intensity of car traffic. We observed that road dust had a significant effect on growth inhibition and death of ostracods, as highest growth inhibition and mortality in the busy areas occurred which also corresponded with highest concentrations of studied elements at these sites. On the other hand, road dust collected in the suburbs of Wrocław did not cause death of H. incongruens and only slightly affected their development. In conclusion, we can state that the chronic toxicity test OSTRACODTOXKIT F™ is a suitable tool to study the impact of road dust on the aquatic ecosystem.

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