Preliminary Evaluation of Protective Efficacy of Inactivated Senecavirus A on Pigs

Senecavirus A (SVA), formerly known as Seneca Valley virus (SVV), causes vesicular symptoms in adult pigs and acute death of neonatal piglets. This pathogen has emerged in major swine producing countries around the world and caused significant economic losses to the pig industry. Thus, it is necessary to develop strategies to prevent and control SVA infection. Herein, an SVA strain (named GD-ZYY02-2018) was isolated from a pig herd with vesicular symptoms in Guangdong province of China in 2018. The present study aimed to carry out the phylogenetic analysis of the GD-ZYY02-2018 strain, determine its pathogenicity in finishing pigs, and assess the protective efficacy of the inactivated GD-ZYY02-2018 strain against virus challenge. The results of phylogenetic analysis showed that the SVA GD-ZYY02-2018 strain belonged to the USA-like strains and had a close genetic relationship with recent Chinese SVA strains. Animal challenge experiment showed that 100-day-old pigs inoculated intranasally with SVA GD-ZYY02-2018 strain developed vesicular lesion, low fever, viremia, and virus shedding in feces. The immunization challenge experiment showed that pigs vaccinated with inactivated GD-ZYY02-2018 strain could produce a high titer of anti-SVA neutralizing antibody and no vesicular lesion, fever, viremia, and virus shedding in feces was observed in vaccinated pigs after challenge with GD-ZYY02-2018 strain, indicating that inactivated GD-ZYY02-2018 could protect finishing pigs against the challenge of homologous virus. In conclusion, preliminary results indicated that inactivated GD-ZYY02-2018 could be used as a candidate vaccine for in-depth research and might be conducive to the prevention and control of SVA infection.

Dermoscopy of cutaneous lymphangioma circumscriptum

Lymphangiomas are congenital lymphatic malformations. They are clinically characterized by clusters of translucent vesicles, and on dermoscopy, yellow lacunae surrounded by pale septa as well as reddish to bluish lacunae have been described. A young male presented with a seven-year history of a vesicular lesion. Dermoscopy revealed multiple white-yellowish well-circumscribed roundish areas (lacunae) surrounded by pale septa. A few lacunae contained blood, which was characteristically accumulated in the lowest part of the lacuna, resulting in an appearance similar to the so-called “hypopyon” of the eye. We suggest a new “dermatologic” metaphoric term to desrcibe this peculiar feature (half-and-half lacuna).

PROFIL HERPES ZOSTER DI POLIKLINIK KULIT DAN KELAMIN RSUP PROF. DR. R. D. KANDOU MANADO PERIODE JANUARI – DESEMBER 2012

Background – Herpes zoster that is also called with shingles, dampa, or cacar ular is a disease caused by the infection of varicella zoster virus (VZV) and affects the skin and mucosa. This infection is a virus reactivation after primer infection characterized with unilateral radicular pain and vesicular lesion that manifests on the skin and its innervation. Objctive – The aim of this research is to find out the profile of outpatient diagnosed with herpes zoster in Dermatovenereology Department RSUP Prof. DR. R. D. Kandou Manado from January to December 2012. Methods – It is a retrospective study that describe several factors related to the subjects, using medical records data from January to December 2012. There were 22 patients evaluated. Result – 2% of 1096 new patients in the period of January to December 2012 were diagnosed as herpes zoster. 73% were 45 – 64 years old, with 54,5% men and 45,4% women, oftalmicus nerve involved counted to 32%, the most common therapy were the combination of Antivirus + Analgesic + Neurovitamin (36,3%). Conclusion – Increasing of the age could be the factor to reactivate the viral infection. The incidence were not affected by sex. Antivirus remain the drug of choice to treat herpes zoster.Key words : herpes zoster – reactivation – virus Abstrak: Latar Belakang – Herpes zoster atau disebut juga dengan shingles, dampa, cacar ular adalah penyakit yang disebabkan oleh infeksi virus varisela – zoster (VVZ) yang menyerang kulit dan mukosa. Infeksi ini merupakan reaktivasi virus yang terjadi setelah infeksi primer yang ditandai dengan adanya nyeri radikuler unilateral serta timbulnya lesi vesikuler yang terbatas pada dermatom yang dipersarafi. Tujuan Penelitian – Mengetahui profil herpes zoster di poliklinik Kulit dan Kelamin RSUP Prof. Dr. R. D. Kandou Manado periode Januari – Desember 2012. Metode – Penelitian ini bersifat deskriptif retrospektif, yaitu mendeskripsikan beberapa faktor yang berhubungan dengan subjek penelitian yang ada. Data penelitian diambil dari rekam medik periode Januari – Desember 2012. Jumlah sampel penelitian sebanyak 22 orang. Hasil Penelitian – Terdapat 2% penderita herpes zoter dari 1096 pasien periode Januari – Desember 2012. Pada umur 45 – 64 tahun terdapat 73% penderita, laki – laki 54,5% dan perempuan 45,5%, lokasi yang sering terkena adalah oftalmikus 32%, serta kombinasi terapi yang sering digunakan adalah Antivirus + Analgesik + Neuronvitamin 36,3%. Kesimpulan – Peningkatan usia dapat memicu reaktivasi virus. Kejadian herpes zoster tidak dipengaruhi oleh jenis kelamin. Obat yang sering digunakan adalah antivirus.Kata kunci : herpes zoster – reaktivasi – virus