Tidal Variation in Cohesive Sediment Distribution and Sensitivity to Flocculation and Bed Consolidation in An Idealized, Partially Mixed Estuary

Particle settling velocity and erodibility are key factors that govern the transport of sediment through coastal environments including estuaries. These are difficult to parameterize in models that represent mud, whose properties can change in response to many factors, including tidally varying suspended sediment concentration (SSC) and shear stress. Using the COAWST (Coupled Ocean-Atmosphere-Wave-Sediment Transport) model framework, we implemented bed consolidation, sediment-induced stratification, and flocculation formulations within an idealized two-dimensional domain that represented the longitudinal dimension of a micro-tidal, muddy, partially mixed estuary. Within the Estuarine Turbidity Maximum (ETM), SSC and median floc diameter varied by a factor of four over the tidal cycle. Downstream of the ETM, the median floc size and SSC were several times smaller and showed less tidal variation (~20% or less). The suspended floc distributions only reached an equilibrium size as a function of SSC and shear in the ETM at peak tidal flow. In general, flocculation increased particle size, which reduced SSC by half in the ETM through increased settling velocity. Consolidation also limited SSC by reduced resuspension, which then limited floc growth through reduced SSC by half outside of the ETM. Sediment-induced stratification had negligible effects in the parameter space examined. Efforts to lessen the computation cost of the flocculation routine by reducing the number of size classes proved difficult; floc size distribution and SSC were sensitive to specification of size classes by factors of 60% and 300%, respectively.

Diversity of Integron- and Culture-Associated Antibiotic Resistance Genes in Freshwater Floc

ABSTRACTClinically important antibiotic resistance genes were detected in culturable bacteria and class 1 integron gene cassettes recovered from suspended floc, a significant aquatic repository for microorganisms and trace elements, across freshwater systems variably impacted by anthropogenic activities. Antibiotic resistance gene cassettes in floc total community DNA differed appreciably in number and type from genes detected in bacteria cultured from floc. The number of floc antibiotic resistance gene cassette types detected across sites was positively correlated with total (the sum of Ag, As, Cu, and Pb) trace element concentrations in aqueous solution and in a component of floc readily accessible to bacteria. In particular, concentrations of Cu and Pb in the floc component were positively correlated with floc resistance gene cassette diversity. Collectively, these results identify suspended floc as an important reservoir, distinct from bulk water and bed sediment, for antibiotic resistance in aquatic environments ranging from heavily impacted urban sites to remote areas of nature reserves and indicate that trace elements, particularly Cu and Pb, are geochemical markers of resistance diversity in this environmental reservoir. The increase in contamination of global water supplies suggests that aquatic environments will become an even more important reservoir of clinically important antibiotic resistance in the future.