An Indo-Pacific coral spawning database

The discovery of multi-species synchronous spawning of scleractinian corals on the Great Barrier Reef in the 1980s stimulated an extraordinary effort to document spawning times in other parts of the globe. Unfortunately, most of these data remain unpublished which limits our understanding of regional and global reproductive patterns. The Coral Spawning Database (CSD) collates much of these disparate data into a single place. The CSD includes 6178 observations (3085 of which were unpublished) of the time or day of spawning for over 300 scleractinian species in 61 genera from 101 sites in the Indo-Pacific. The goal of the CSD is to provide open access to coral spawning data to accelerate our understanding of coral reproductive biology and to provide a baseline against which to evaluate any future changes in reproductive phenology.

A possible stimulus to induce SPAWNING in Ezo abalone Haliotis discus hannai during stormy weather: Fenton reaction induces spawning behavior

Synchronous spawning is an important behavior to increase fertilization success of invertebrates with external fertilization. Previous work has shown that it is possible to induce spawning behavior using free radicals in tank experiments, but the stimulus for spawning in the wild is not fully understood. Ezo abalone Haliotis discus hannai mainly spawn during stormy weather. Rainwater contains H2O2 and iron (II) ions (Fe2+). We propose that during stormy weather water layers in the ocean are mixed and the surface layer containing H2O2 and Fe2+ interacts with the ocean bottom; this leads to conditions suitable for the Fenton reaction to occur. Hydroxyl radicals (·OH) are generated during the oxidization of Fe2+ by H2O2 and we hypothesized these induce spawning behavior of abalone in the wild. This study observed that H. discus hannai released eggs after salinity decreased due to the rainfall during stormy conditions. In addition, our tank experiment demonstrated that ·OH generated by the Fenton reaction induced synchronous spawning behavior between the sexes. This study provides a new hypothesis about control of synchronous spawning in H. discus hannai and the results could be applicable to other invertebrates.

Environmental factors explain spawning day deviation from full moon in the scleractinian coral Acropora

Broadcast-spawning scleractinian corals annually release their gametes with high levels of synchrony, both within and among species. However, the timing of spawning can vary inter-annually. In particular, the night of spawning relative to the full moon phase can vary considerably among years at some locations. Although multiple environmental factors can affect the night of spawning, their effects have not been quantitatively assessed at the multi-regional level. In this study, we analysed environmental factors that are potentially correlated with spawning day deviation, in relation to the full moon phase, in Acropora corals inhabiting seven reefs in Australia and Japan. We accordingly found that sea surface temperature and wind speed within one to two months prior to the full moon of the spawning month were strongly correlated with spawning day deviations. In addition, solar flux had a weak effect on the night of spawning. These findings indicate that Acropora have the capacity to adjust their development and physiology in response to environmental factors for fine-tuning the timing of synchronous spawning, thereby maximizing reproductive success and post-fertilization survival.

Eyes in Staurozoa (Cnidaria): a review

The presence of dark pigment spots associated with primary tentacles (or structures derived from them, i.e., rhopalioids) in Staurozoa was recently overlooked in a study on the evolution of cnidarian eyes (defined as a “region made of photoreceptor cells adjacent to pigment cells”, irrespective of image formation, i.e., including all photoreceptive organs). Review of old and recent literature on Staurozoa shows that dark pigment spots are present in virtually all species ofManania, as well as some species ofHaliclystus,Stylocoronella, and probablyCalvadosia. The known ultrastructure of ocelli seems to be compatible with light perception, but no immediate response to changes in light intensity have been observed in the behavior of staurozoans. Therefore, although further studies addressing photic behavior are required, we discuss an earlier hypothesis that the dark spots in some stauromedusae may be related to synchronous spawning, as well as the possible sensorial function of rhopalioids. Observations summarized here suggest a possible ninth independent origin of eyes in Cnidaria, within a lineage of benthic medusae. Alternatively, documented similarity across medusae of Cubozoa, Scyphozoa, and Staurozoa—with eyes being topologically associated with primary tentacles in each of these taxa—could indicate shared ancestry and a single origin of eyes in this clade known as Acraspeda. Information on Staurozoa, one of the least studied groups within Cnidaria, is often neglected in the literature, but correctly recognizing the characters of this class is crucial for understanding cnidarian evolution.

Eyes in Staurozoa (Cnidaria): a review

The presence of dark pigment spots associated with primary tentacles (or structures derived from them, i.e., rhopalioids) in Staurozoa was recently overlooked in a study on the evolution of cnidarian eyes (defined as a “region made of photoreceptor cells adjacent to pigment cells”, irrespective of image formation, i.e., including all photoreceptive organs). Review of old and recent literature on Staurozoa shows that dark pigment spots are present in virtually all species of Manania, as well as some species of Haliclystus, Stylocoronella, and probably Calvadosia. The known ultrastructure of ocelli seems to be compatible with light perception, but no immediate response to changes in light intensity have been observed in the behavior of staurozoans. Therefore, although further studies addressing photic behavior are required, we discuss an earlier hypothesis that the dark spots in some stauromedusae may be related to synchronous spawning, as well as the possible sensorial function of rhopalioids. Observations summarized here suggest a possible ninth independent origin of eyes in Cnidaria, within a lineage of benthic medusae. Alternatively, documented similarity across medusae of Cubozoa, Scyphozoa, and Staurozoa – with eyes being topologically associated with primary tentacles in each of these taxa – could indicate shared ancestry and a single origin of eyes in this clade known as Acraspeda. Information on Staurozoa, one of the least studied groups within Cnidaria, is often neglected in the literature, but correctly recognizing the characters of this class is crucial for understanding cnidarian evolution.

Eyes in Staurozoa (Cnidaria): a review

The presence of dark pigment spots associated with primary tentacles (or structures derived from them, i.e., rhopalioids) in Staurozoa was recently overlooked in a study on the evolution of cnidarian eyes (defined as a “region made of photoreceptor cells adjacent to pigment cells”, irrespective of image formation, i.e., including all photoreceptive organs). Review of old and recent literature on Staurozoa shows that dark pigment spots are present in virtually all species of Manania, as well as some species of Haliclystus, Stylocoronella, and probably Calvadosia. The known ultrastructure of ocelli seems to be compatible with light perception, but no immediate response to changes in light intensity have been observed in the behavior of staurozoans. Therefore, although further studies addressing photic behavior are required, we discuss an earlier hypothesis that the dark spots in some stauromedusae may be related to synchronous spawning, as well as the possible sensorial function of rhopalioids. Observations summarized here suggest a possible ninth independent origin of eyes in Cnidaria, within a lineage of benthic medusae. Alternatively, documented similarity across medusae of Cubozoa, Scyphozoa, and Staurozoa – with eyes being topologically associated with primary tentacles in each of these taxa – could indicate shared ancestry and a single origin of eyes in this clade known as Acraspeda. Information on Staurozoa, one of the least studied groups within Cnidaria, is often neglected in the literature, but correctly recognizing the characters of this class is crucial for understanding cnidarian evolution.

Eyes in Staurozoa (Cnidaria): a review

The presence of dark pigment spots associated with primary tentacles (or structures derived from them, i.e., rhopalioids) in Staurozoa was recently overlooked in a study on the evolution of cnidarian eyes (defined as a “region made of photoreceptor cells adjacent to pigment cells”, irrespective of image formation, i.e., including all photoreceptive organs). Review of old and recent literature on Staurozoa shows that dark pigment spots are present in virtually all species of Manania, as well as some species of Haliclystus, Stylocoronella,and probably Calvadosia. Based on our review, we support the hypothesis that these dark spots may be related to synchronous spawning, and that rhopalioids have both adhesive and sensorial functions. Observations summarized here suggest a possible ninth independent origin of eyes in Cnidaria, within a lineage of benthic medusae. Alternatively, documented similarity across Cubozoa, Scyphozoa, and Staurozoa – with eyes being topologically associated with primary tentacles in each of these taxa – could indicate shared homology and a single origin of eyes in this clade known as Acraspeda. Information on Staurozoa, one of the least studied groups within Cnidaria, is often neglected in the literature, but correctly recognizing the characters of this classis crucial for understanding cnidarian evolution.

The reef-building coral Acropora conditionally hybridize under sperm limitation

Multi-specific synchronous spawning risks both sperm limitation, which reduces fertilization success, and hybridization with other species. If available sperm of conspecifics are limited, hybridization with heterospecific sperm could be an alternative. Some species of the reef-building coral Acropora produce hybrid offspring in vitro , and therefore hybridization between such species does sometimes occur in nature. Here, we report that the interbreeding species Acropora florida and A. intermedia preferentially bred with conspecifics at optimal gamete concentrations (10 6 cells ml −1 ), but when sperm concentration was low (10 4 cells ml −1 ), A . florida eggs displayed an increased incidence of fertilization by sperm of A . intermedia . However, A . intermedia eggs never crossed with heterospecific sperm, regardless of gamete concentrations. It appears that A . florida eggs conditionally hybridize with heterospecific sperm; in nature, this would allow A . florida to cross with later-spawning species such as A . intermedia . These results indicate that hybridization between some Acropora species could occur in nature according to the number of available sperm, and the choice of heterospecific sperm for fertilization could be one of the fertilization strategies in the sperm-limited condition.