Abstract
Using BEAST v1.X, we constructed a credible timetree of 115 specimens of Odonata and five species of Ephemeroptera (Paleoptera; Pterygota) and two species of Archaeognatha and three species of Zygentoma (Apterygota). 88 specimens we ourselves analyzed were collected from the Ryukyu islands, Taiwan, Japan, and China, and the resting sequence data were mostly from whole mitochondrial data found in GenBank / DDJB. The combined gene (not concatenated gene) analysis of the mitochondrial COI (795 bp), COII (548 bp), and 16S rRNA (517 bp), and the nuclear 28S rRNA (825 bp) were performed. Using the calibration function of BEAST v1.X, the timetree was constructed by applying a 1.55 Ma geological event (isolation of the Ryukyu islands from China), in addition to chronologically robust fossil dates ranging from 400 Ma for Archaeognatha, 300 Ma for Ephemeroptera, and 200 Ma for Odonata and to 1.76 Ma for Calopterygidae, for a total of 13 calibration points (event: 6, fossil: 12; Quaternary 7, pre Quaternary 11). The resultant timetree showed that molecular clock was not uniformly progressed, and the base substitution rate has exponentially increased from ca. 20 Ma to the Recent by over an order of magnitude. Our new and attractive finding indicates that the Quaternary severe climatic change including a start of glacial and interglacial cycle might have resulted in the extensive radiation and speciation of Odonata, and consequently increased the biodiversity. C4 pores generated in the Miocene effectively decreased atmospheric CO2, and triggered the Quaternary glaciation. Another peak of base substitution rate was found in the Carboniferous time around 320 Ma, and this may be analogous to the late Paleozoic icehouse. This glaciation has been triggered by the development of terrestrial plants to form thick coal layers, because this process also reduced the atmospheric CO2.