Improving Ecological Functions and Ornamental Values of Traditional Pear Orchard by Co-Planting of Green Manures of Astragalus sinicus L. and Lathyrus cicera L.

Traditional orchards received little attention in ecology. In order to enhance the ecological function of traditional pear orchard, it is an effective strategy to co-plant the ornamental green manure (GM) under the pear forest. In this study, two kinds of GM, i.e., Astragalus sinicus L. (AS) and Lathyrus cicera L. (LC), were co-planted in pear tree orchard to elevate its landscape benefits of spatiotemporal distribution of flowers, the nutrient benefits and oxygen production. The results showed that the flower height of AS and LC arrange between 20~30 cm, and the flowering period covers the March. LC has a large number of flowers, a small area of single flower, and high yield of fresh grass. AS has a small number of flowers, a large area of single flower, and low yield of a single fresh grass. Among them, 35% AS + 65% LC and 50% AS + 50% LC are more suitable in achieving the well tourism value and potential good production of pear orchard. Nutrient accumulation, total carbon fixation and oxygen production, flower number of 35% AS + 65% LC are larger than other treatments, while the flower period of 50% AS + 50% LC is longest. This study proposed a “win-win” GM planting strategy for sustainable orchard development, by enhancing ecology functions and the landscaped value of the traditional fruit orchard.

Biocatalytic Production of Aldehydes: Exploring the Potential of Lathyrus cicera Amine Oxidase

Aldehydes are a class of carbonyl compounds widely used as intermediates in the pharmaceutical, cosmetic and food industries. To date, there are few fully enzymatic methods for synthesizing these highly reactive chemicals. In the present work, we explore the biocatalytic potential of an amino oxidase extracted from the etiolated shoots of Lathyrus cicera for the synthesis of value-added aldehydes, starting from the corresponding primary amines. In this frame, we have developed a completely chromatography-free purification protocol based on crossflow ultrafiltration, which makes the production of this enzyme easily scalable. Furthermore, we determined the kinetic parameters of the amine oxidase toward 20 differently substituted aliphatic and aromatic primary amines, and we developed a biocatalytic process for their conversion into the corresponding aldehydes. The reaction occurs in aqueous media at neutral pH in the presence of catalase, which removes the hydrogen peroxide produced during the reaction itself, contributing to the recycling of oxygen. A high conversion (>95%) was achieved within 3 h for all the tested compounds.

Alternative Crops for Adaptation to Climate Change: The Importance of Conserving the Diversity of Lathyrus cicera L. Landraces Adapted to the Morocco Mountains

A Global climate change has raised serious concerns about food security and the sustainability of agriculture, particularly in developing regions of the world. In response to these concerns, attention should be called to the global importance of conservation of some neglected and underutilized crops, such as Lathyrus species, which are nutrient-rich and already adapted to harsh environments and low-input agriculture. L. cicera L., known in Morocco as ‘ikiker’, ‘kiker’ or ‘ichicher’, is marginally cultivated in the region. Landraces of this crop species, which are maintained locally by traditional agricultural practices, correspond to ecotypes adapted to local agroclimatic conditions. We have surveyed the traditional cultivation sites of this crop to identify specific associated agroecosystems in the Middle and High Atlas Mountains of Morocco. We have evaluated the diversity of ecotypes of L. cicera L. by a set of characters associated with the socioeconomic and agromorphological aspects of their cultivation. The results confirmed that their cultivation is very old in the area, and that its maintenance until today is important as the local farmers have started to master the uses for human and animal food. In addition, from a biology point of view, we have demonstrated the existence of variability depending on the trait considered but which demonstrates a differentiation between the ecotypes. From adaptive potential of these ecotypes with respect to tolerance to aridity and increased temperatures, the ecotypes studied showed promising prospects for selection. Thus, despite the limitation of the territory and the regression of the culture, the studied ecotypes have a very interesting stand. This result can be explained by cultural practices. These ecotypes are maintained in traditional agroecosystems which play the role of conservatory of the neglected resources. Therefore, the conservation of these genetic resources depends on the conservation of the traditional agroecosystem and local knowledge.

Partial Resistance Against Erysiphe pisi and E. trifolii Under Different Genetic Control in Lathyrus cicera: Outcomes from a Linkage Mapping Approach

Powdery mildew infections are among the most severe foliar biotrophic fungal diseases in grain legumes. Several accessions of Lathyrus cicera (chickling pea) show levels of partial resistance to Erysiphe pisi, the causal agent of pea powdery mildew, and to E. trifolii, a powdery mildew pathogen recently confirmed to infect pea and Lathyrus spp. Nevertheless, the underlying L. cicera resistance mechanisms against powdery mildews are poorly understood. To unveil the genetic control of resistance against powdery mildews in L. cicera, a recombinant inbred line population segregating for response to both species was used in resistance linkage analysis. An improved L. cicera genetic linkage map was used in this analysis. The new higher-density linkage map contains 1,468 polymorphic loci mapped on seven major and two minor linkage groups, covering a total of 712.4 cM. The percentage of the leaf area affected by either E. pisi or E. trifolii was recorded in independent screenings of the recombinant inbred line population, identifying a continuous range of resistance-susceptibility responses. Distinct quantitative trait loci (QTLs) for partial resistance against each pathogen were identified, suggesting different genetic bases are involved in the response to E. pisi and E. trifolii in L. cicera. Moreover, through comparative mapping of L. cicera QTL regions with the pea reference genome, candidate genes and pathways involved in resistance against powdery mildews were identified. This study extended the previously available genetic and genomic tools in Lathyrus species, providing clues about diverse powdery mildew resistance mechanisms useful for future resistance breeding of L. cicera and related species.