Penggunaan sedasi inhalasi N 2 O-O 2 pada penatalaksanaan marsupialisasi ranula rongga mulut anak anxiety patient

Inhalation sedation becomes the main choice in the dental treatment of children whoovercome have any kind of the fearness, concerned with pain in the treatment of theirteeth. Inhalation sedation with N2O-Ocauses sedation situation that accompanies byanalgesia to the conscious patients by inhaling the mixture of oxide nitrogen (N2O)with oxygen (O), that was known as psychosedation or concious sedation technique.Ranula is a mucocel that developed at the base of the mouth, unilateral, bluish, andthe shape like frog stomach. On this paper, will be esplained about N2O-O2inhalationsedation technique that concider the condition for the patient, using short time andcan be recovered immediately. A 9 year old girl diagnosed by ranula sublingualis onthe cavity of the mouth was very anxiety and fear in this treatment. The ranula treatedwith marsupialitation and was supervised by inhalation sedation of N2O-O2 in theSpecial Dental Care Hasan Sadikin Hospital Bandung. The result showed thatinhalation sedation with N2O-O2 was very very useful to reduce anxiety and fear tocertain patients with minimal side-effect. So it was very important to a dentist to knowthe selection of the patient, equipment and the technique of inhalation sedationadequately

Helicobacter pyloricytotoxin VacA increases alkaline secretion in gastric epithelial cells

Human infection by the bacterium Helicobacter pylori (Hp) may lead to severe gastric diseases by an ill-understood process involving several virulence factors. Among these, the cytotoxin VacA is associated with higher tissue damage. In this study, the isolated frog stomach model was used to characterize the acute effects of VacA on the gastric epithelium. Our results show that VacA partially inhibits gastric acid output by increasing HCO[Formula: see text] efflux. Experiments conducted with double-barrelled pH or Cl−-selective microelectrodes on surface epithelial gastric cells (SECs) and single gastric glands show that VacA does not impair the activity of the oxyntic cells but renders the apical membrane of SECs more permeable to HCO[Formula: see text] and Cl−. Inhibition of this permeation by 5-nitro-2-(3-phenylpropylamino) benzoic acid indicates that this may be due to the formation of anion-selective pores by the toxin. We suggest that VacA-dependent HCO[Formula: see text] efflux from SECs improves the environmental conditions (pH, CO2concentration) of the niche parasitized by Hp, that is the gastric surface. This may favor Hp persistence in the tissue and the secondary development of a chronic inflammation.

Effect of histamine on the basolateral K+ conductance of frog stomach oxyntic cells and surface epithelial cells

The transepithelial potential difference (Vt) and resistance (Rt) and the basolateral cell membrane potential (Vs) of oxyntic cells (OC) and surface epithelial cells (SEC) were measured in isolated stomachs of Rana esculenta. At rest, Vs of OC and SEC was virtually identical [-66.3 +/- 4.5 (SD) (n = 10) and -67.3 +/- 5.9 mV (n = 9)] and both cells responded to increasing serosal K+ concentration from 4 to 13 mmol/l with virtually the same depolarization (delta Vs,K) of +16.2 +/- 2.0 and +16.0 +/- 2.9 mV, respectively, while Vt declined by approximately half as much. Histamine (0.1 mmol/l) reduced Vt and Rt and increased the voltage divider ratio in both cell types, indicating a fall in basolateral membrane resistance. In the OC, this increase was neither associated with a significant alteration of Vs nor with a change in delta Vs,K. In the SEC, however, histamine markedly increased Vs to -75.5 +/- 7.3 mV (n = 9) as well as delta Vs,K to +18.5 +/- 2.6 mV, which was paralleled by an increase in delta Vt,K from 9.8 +/- 3.9 to +12.8 +/- 4.2 mV. The data indicate that 1) both OC and SEC respond to histamine, 2) both OC and SEC contain a basolateral K+ conductance that increases under histamine (in OC probably, in parallel with other ion conductances), and 3) in Rana esculenta the SEC contribute substantially to Vt.