Evaluation of the antioxidant potential of methanol extract of Chenopodium botrys L. (Amaranthaceae) collected from six different locations in Republic of Macedonia was performed. Several methods were used for testing the antioxidative activity: 1) 2,2-diphenyl1-picrylhydrazyl (DPPH) radical scavenging assay, 2) ferric reduction power assay (FRAP), 3) inhibition of H2 O2 activity, 4) non-sitespecific hydroxyl radical-catalyzed 2-deoxy-D-ribose degradation (NSSOH) and 5) site-specific hydroxyl radical-catalyzed 2-deoxy-D-ribose degradation (SSOH). The IC50 values ranged from 0.26-3.10 mg/mL, 3.01-12.71 mg/mL and 2.60-12.29 mg/mL, for DPPH, NSSOH and SSOH assays, respectively. The H2 O2 inhibition activity and the ferric reducing power capacity were from 28.84-46.56% and 26.14- 43.40%, respectively. The obtained data establish the antioxidant potency in concentration-dependent manner. Additionally, total phenols (TPC) and total flavonoid content (TFC) were determined. The estimated values ranged from 27.77-71.25 mg GAE/g DW and from 7.35- 16.33 mg QE/g DW, respectively