allele discovery
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Genetics ◽  
2019 ◽  
Vol 213 (2) ◽  
pp. 595-613 ◽  
Author(s):  
Alex Hemshrot ◽  
Ana M. Poets ◽  
Priyanka Tyagi ◽  
Li Lei ◽  
Corey K. Carter ◽  
...  


BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Kaitlin Stouffer ◽  
Michael Nahorski ◽  
Pablo Moreno ◽  
Nivedita Sarveswaran ◽  
David Menon ◽  
...  


2017 ◽  
Vol 70 (6) ◽  
pp. 381-399 ◽  
Author(s):  
Matthew R. Semler ◽  
Roger W. Wiseman ◽  
Julie A. Karl ◽  
Michael E. Graham ◽  
Samantha M. Gieger ◽  
...  


2017 ◽  
Vol 68 (20) ◽  
pp. 5453-5470 ◽  
Author(s):  
Roswitha Schmickl ◽  
Sarah Marburger ◽  
Sian Bray ◽  
Levi Yant


2017 ◽  
Vol 18 (7) ◽  
pp. 673-685 ◽  
Author(s):  
Yao Yang ◽  
Mariana R Botton ◽  
Erick R Scott ◽  
Stuart A Scott


2017 ◽  
Vol 69 (4) ◽  
pp. 211-229 ◽  
Author(s):  
Julie A. Karl ◽  
Michael E. Graham ◽  
Roger W. Wiseman ◽  
Katelyn E. Heimbruch ◽  
Samantha M. Gieger ◽  
...  


2016 ◽  
Author(s):  
Trent M. Prall ◽  
Michael E. Graham ◽  
Julie A. Karl ◽  
Roger W. Wiseman ◽  
Adam J. Ericsen ◽  
...  

ABSTRACTKiller cell Immunoglobulin-like Receptors (KIRs) modulate disease progression of pathogens including HIV, malaria, and hepatitis C. Cynomolgus and rhesus macaques are widely used as nonhuman primate models to study human pathogens and so considerable effort has been put into characterizing their KIR genetics. However, previous studies have relied on cDNA cloning and Sanger sequencing that lacks the throughput of current sequencing platforms. In this study, we present a high throughput, full-length allele discovery method utilizing PacBio circular consensus sequencing (CCS). We also describe a new approach to Macaque Exome Sequencing (MES) and the development of the Rhexome1.0, an adapted target capture reagent that includes macaque-specific capture probesets. By using sequence reads generated by whole genome sequencing (WGS) and MES to inform primer design, we were able to increase the sensitivity of KIR allele discovery. We demonstrate this increased sensitivity by defining nine novel alleles within a cohort of Mauritian cynomolgus macaques (MCM), a geographically isolated population with restricted KIR genetics that was thought to be completely characterized. Finally, we describe an approach to genotyping KIRs directly from sequence reads generated using WGS/MES reads. The findings presented here expand our understanding of KIR genetics in MCM by associating new genes with all eight KIR haplotypes and demonstrating the existence of at least one KIR3DS gene associated with every haplotype.



2016 ◽  
Author(s):  
Julie A. Karl ◽  
Michael E. Graham ◽  
Roger W. Wiseman ◽  
Katelyn E. Heimbruch ◽  
Samantha M. Gieger ◽  
...  

ABSTRACTVery little is currently known about the major histocompatibility complex (MHC) region of cynomolgus macaques (Macaca fascicularis; Mafa) from Chinese breeding centers. We performed comprehensive MHC class I haplotype analysis of 100 cynomolgus macaques from two different centers, with animals from different reported original geographic origins (Vietnamese, Cambodian, and Cambodian/Indonesian mixed-origin). Many of the samples were of known relation to each other (sire, dam, and progeny sets), making it possible to characterize lineage-level haplotypes in these animals. We identified 52 Mafa-A and 74 Mafa-B haplotypes in this cohort, many of which were restricted to specific sample origins. We also characterized full-length MHC class I transcripts using Pacific Biosciences (PacBio) RS II single-molecule real-time (SMRT) sequencing. This technology allows for complete read-through of unfragmented MHC class I transcripts (~1,100 bp in length), so no assembly is required to unambiguously resolve novel full-length sequences. Overall, we identified 313 total full-length transcripts in a subset of 72 cynomolgus macaques from these Chinese breeding facilities; 131 of these sequences were novel and an additional 116 extended existing short database sequences to span the complete open reading frame. This significantly expands the number of Mafa-A, Mafa-B, and Mafa-I full-length alleles in the official cynomolgus macaque MHC class I database. The PacBio technique described here represents a general method for full-length allele discovery and genotyping that can be extended to other complex immune loci such as MHC class II, killer immunoglobulin-like receptors, and Fc gamma receptors.



2015 ◽  
Vol 76 (12) ◽  
pp. 891-896 ◽  
Author(s):  
Catherine J. Westbrook ◽  
Julie A. Karl ◽  
Roger W. Wiseman ◽  
Suzanne Mate ◽  
Galina Koroleva ◽  
...  


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