Flexible multiplane structured illumination microscope with a four-camera detector

Fluorescence microscopy provides an unparalleled tool for imaging biological samples. However, producing high-quality volumetric images quickly and without excessive complexity remains a challenge. Here, we demonstrate a simple multi-camera structured illumination microscope (SIM) capable of simultaneously imaging multiple focal planes, allowing for the capture of 3D fluorescent images without any axial movement of the sample. This simple setup allows for the rapid acquisition of many different 3D imaging modes, including 3D time lapses, high-axial-resolution 3D images, and large 3D mosaics.

Vision metrology and Structure from Motion for archaeological heritage 3D reconstruction: a Case Study of various Roman mosaics

<p class="Abstract">Vision metrology and computer vision can be successfully used for archaeological heritage 3D reconstruction in very high precision 3D measurement projects. Of those archaeological objects requiring very accurate measurements (&lt;1 mm), ancient mosaics comprise some of the most important. The aim of this paper is to assess the photogrammetric/computer vision approach in a vision metrology context as part of a 3D mosaics survey. In order to evaluate the optimal photogrammetric/computer vision workflow in this work, three different surveys were performed on three mosaics of different sizes and locations. Two of these are stored at the <em>Antonino Salinas</em> Regional Archaeological Museum in Palermo (Italy) and the other is located at the <em>Baglio Anselmi</em> Regional Archaeological Museum in Marsala (Italy). The mosaics survey was undertaken in order to obtain a very detailed 3D model and a full-scale ortho-image (scale 1:1), which would be useful for documentation and restoration processes. The research involved an evaluation of the potential and the related issues of the photogrammetric/computer vision approach for 3D mosaic documentation, particularly regarding the issue of camera calibration.</p>

Morphable 3D-Mosaics: A Hybrid Framework for Photorealistic Walkthroughs of Large Natural Environments

This paper presents a hybrid (geometry- & image-based) framework suitable for providing photorealistic walkthroughs of large, complex outdoor scenes at interactive frame rates. To this end, based just on a sparse set of real stereoscopic views from the scene, a set ofmorphable 3D-mosaicsis automatically constructed first, and then, during rendering, a continuous morphing between those 3D-mosaics that are nearby to the current viewpoint is taking place. The morphing is both photometric, as well as geometric, while we also ensure that it proceeds in a physically valid manner, thus remaining transparent to the user. The effectiveness of our framework has been demonstrated in the 3D visual reconstruction of the Samaria Gorge in Crete, which is one of the largest and most beautiful gorges in Europe.