In this work, microwave treated Gardenia jasminoides leaves (MTGL) were employed to remove Direct Red-28 (DR) dye from aqueous medium. Most of DR dye contents removed within 30 minutes at pH 2 and 350 ppm dye concentration by 0.02 mg MTGL. Maximum dye adsorbed by MTGL (88.50 mg/g) was approximately triple than non treated simple Gardenia jasminoides leaves (34.13 mg/g). Adsorption modelling of equilibrium data indicated that removal of DR dye by MTGL followed Langmuir, Freundlich and pseudo-second order kinetic models, having exothermic nature. Desorption studies indicated the reusability of MTGL on larger scale. So it is clear that Gardenia jasminoide leaves can be used on larger scale for anionic dye removal after treatment with formalin in efficient manner.