An Artificial Sweating System for Sweat Sensor Testing Applications

This research proposes a completely automated, computer-controlled fluid mixing and dispensing system, which is suitable for testing sweat sensing devices, as an alternative to requiring human trials during the development phase of a sweat sensor device. An arm mold was designed and implemented with dragon skin and pores to simulate sweating action. The relay controlled mixing tanks allow for the different concentration of fluid solutions at various rates of fluid dispensing through pores. The onboard single board computer controls a dozen electronic relays and it switches and presents an easy to use graphical user interface to allow end users to conduct the experiments with ease and not require further programming. With the recent advances in sweat sensors, this platform offers a unique way of testing sensing devices during development, allowing for researchers to focus on their design parameters one at a time before actual validation through human trials are conducted. The current device can provide sweat rates from 1 µL/min to 500 µL/min. Furthermore, concentrations of 10 mM up to 200 mM of salt concentrations were able to be repeatedly produced. In an ANOVA test with salt concentrations varying from 40–60 mM, a p-value of 0.365 shows that the concentration does not have any effect on the flow rate. Similarly, a p-value of 0.329 and 0.167 for different relative humidity and temperature shows that the system does not present a statistical difference. Lastly, when the interactions among all the factors were considered, a p-value of 0.416 clearly presents that the system performance is insensitive to different factors, thus validating the system reliability.

A Low-Cost, Accurate, and High-Precision Fluid Dispensing System for Microscale Application

We present here the development of a low-cost, accurate, and precise fluid dispensing system. It can be used with peristaltic or any other pump to improve the flow characteristics. The dispensing system has a range of 1 to 100 µL with accuracy of ~99.5% and standard deviation at ~150 nL over the entire range. The system developed does not depend on the accuracy or precision of the driving pump; therefore, any positive displacement pump can be used to get similar accuracy and precision, which gives an opportunity to reduce the cost of the system. The dispensing system does not require periodic calibration and can also be miniaturized for microfluidic application. Although primarily designed for aqueous liquid, it can be extended for different nonconductive liquids as well with modifications. The unit is further used for near real-time measurement of lactate from microdialysate. The individual components can easily be made disposable or sterilized for use in biomedical applications.