The chitosan resin forming with commercial detergent solution was used as adsorbent for removal of the methylene blue. The effect of the amount of adsorbent was thoroughly investigated through batch adsorption system. The percent adsorption of methylene blue increased in the same direction as the amount of chitosan resin. The experimental result showed that adsorption capacity onto 0.4 g of chitosan resin was 9.1 mg/g. The Langmuir and the Freundlich adsorption isotherms were applied to describe the methylene blue uptake, which could be explained by Freundlich adsorption isotherm onto chitosan resin. Single-state batch adsorption design of methylene blue onto chitosan resin has been studied, using on the Freundlich isotherm equation.