proteomic fingerprinting
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Author(s):  
Jasmin Renz ◽  
Elena L. Markhaseva ◽  
Silke Laakmann ◽  
Sven Rossel ◽  
Pedro Martinez Arbizu ◽  
...  

2020 ◽  
Vol 14 (10) ◽  
pp. e0008849
Author(s):  
Rolando A. Gittens ◽  
Alejandro Almanza ◽  
Kelly L. Bennett ◽  
Luis C. Mejía ◽  
Javier E. Sanchez-Galan ◽  
...  

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry is an analytical method that detects macromolecules that can be used for proteomic fingerprinting and taxonomic identification in arthropods. The conventional MALDI approach uses fresh laboratory-reared arthropod specimens to build a reference mass spectra library with high-quality standards required to achieve reliable identification. However, this may not be possible to accomplish in some arthropod groups that are difficult to rear under laboratory conditions, or for which only alcohol preserved samples are available. Here, we generated MALDI mass spectra of highly abundant proteins from the legs of 18 Neotropical species of adult field-collected hard ticks, several of which had not been analyzed by mass spectrometry before. We then used their mass spectra as fingerprints to identify each tick species by applying machine learning and pattern recognition algorithms that combined unsupervised and supervised clustering approaches. Both Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) classification algorithms were able to identify spectra from different tick species, with LDA achieving the best performance when applied to field-collected specimens that did have an existing entry in a reference library of arthropod protein spectra. These findings contribute to the growing literature that ascertains mass spectrometry as a rapid and effective method to complement other well-established techniques for taxonomic identification of disease vectors, which is the first step to predict and manage arthropod-borne pathogens.


Malacologia ◽  
2020 ◽  
Vol 63 (1) ◽  
pp. 131
Author(s):  
Thomas Wilke ◽  
Jasmin Renz ◽  
Torsten Hauffe ◽  
Diana Delicado ◽  
Janna Peters

2019 ◽  
Vol 203 ◽  
pp. 103390 ◽  
Author(s):  
Luiz Marcelo Ribeiro Tomé ◽  
Fernanda Badotti ◽  
Gabriella Borba Netto Assis ◽  
Paula Luize Camargos Fonseca ◽  
Genivaldo Alves da Silva ◽  
...  

PROTEOMICS ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1800290 ◽  
Author(s):  
Christina Maria Bredtmann ◽  
Jürgen Krücken ◽  
Jayaseelan Murugaiyan ◽  
Alice Balard ◽  
Heribert Hofer ◽  
...  

2018 ◽  
Vol 40 (2) ◽  
pp. 266-271 ◽  
Author(s):  
María Jesús Lerma-García ◽  
Maria Nicoletti ◽  
Ernesto Francisco Simó-Alfonso ◽  
Pier Giorgio Righetti ◽  
Elisa Fasoli

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0195148 ◽  
Author(s):  
Makan Golizeh ◽  
Carlos E. Melendez-Pena ◽  
Brian J. Ward ◽  
Sahar Saeed ◽  
Cynthia Santamaria ◽  
...  

2017 ◽  
Vol 164 ◽  
pp. 52-58 ◽  
Author(s):  
María Vergara-Barberán ◽  
María Jesús Lerma-García ◽  
Maria Nicoletti ◽  
Ernesto Francisco Simó-Alfonso ◽  
José Manuel Herrero-Martínez ◽  
...  

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