Proteomic fingerprinting for the fast and accurate identification of species in the Polyporoid and Hymenochaetoid fungi clades

2019 ◽  
Vol 203 ◽  
pp. 103390 ◽  
Author(s):  
Luiz Marcelo Ribeiro Tomé ◽  
Fernanda Badotti ◽  
Gabriella Borba Netto Assis ◽  
Paula Luize Camargos Fonseca ◽  
Genivaldo Alves da Silva ◽  
...  
Keyword(s):  
Accurate Identification ◽  
Identification Of Species ◽  
Proteomic Fingerprinting

Tree kangaroo molecular systematics based on partial cytochrome b sequences: are Matschie's tree kangaroo (Dendrolagus matschiei) and Goodfellow's tree kangaroo (D. goodfellowi buergersi) sister taxa?

2012 ◽  
Vol 34 (1) ◽  
pp. 18 ◽  
Author(s):  
Thomas J. McGreevy ◽  
Lisa Dabek ◽  
Thomas P. Husband
Keyword(s):  
Mitochondrial Dna ◽  
Papua New Guinea ◽  
Cytochrome B ◽  
Molecular Systematics ◽  
Phylogenetic Analyses ◽  
New Guinea ◽  
Critically Endangered ◽  
Evolutionary Relationships ◽  
Accurate Identification ◽  
Identification Of Species

New Guinea tree kangaroos (Dendrolagus spp.) are unique arboreal macropodid marsupials mainly listed as critically endangered or endangered. The molecular systematics of Dendrolagus has not been fully resolved and is critical for the accurate identification of species and their evolutionary relationships. Matschie’s tree kangaroo (D. matschiei) and Goodfellow’s tree kangaroo (D. goodfellowi buergersi) share numerous morphological, physiological, and behavioural traits. We analysed the partial mitochondrial DNA cytochrome b gene for D. matschiei (n = 67), D. g. buergersi (n = 8), D. goodfellowi unidentified ssp. (n = 8), golden-mantled tree kangaroo (D. g. pulcherrimus; n = 1), and two additional New Guinea Dendrolagus taxa to determine whether D. matschiei and D. g. buergersi are sister taxa. D. matschiei and D. g. buergersi were not placed as sister taxa in our phylogenetic analyses; however, we were unable to analyse a known sample from a D. g. goodfellowi. We found initial genetic evidence that D. matschiei and the Lowland tree kangaroo (D. spadix) are sister taxa – they may have diverged after the formation of the Huon Peninsula of Papua New Guinea. Our results also support the elevation of D. g. pulcherrimus to a full species. An improved understanding of Dendrolagus molecular systematics will contribute substantially to their conservation.

Shedding new light on old species identifications: morphological and genetic evidence suggest a need for conservation status review of the critically endangered bat, Saccolaimus saccolaimus

2009 ◽  
Vol 36 (6) ◽  
pp. 496 ◽  
Author(s):  
Damian J. Milne ◽  
Felicity C. Jackling ◽  
Manpreet Sidhu ◽  
Belinda R. Appleton
Keyword(s):  
Threatened Species ◽  
Conservation Status ◽  
Critically Endangered ◽  
Similar Species ◽  
Museum Specimens ◽  
Museum Collections ◽  
Species Classification ◽  
Accurate Identification ◽  
Identification Of Species ◽  
Vital Component

Information based on the accurate identification of species is a vital component for achieving successful outcomes of biodiversity conservation and management. It is difficult to manage species that are poorly known or that are misidentified with other similar species. This is particularly problematic for rare and threatened species. Species that are listed under endangered species classification schemes need to be identified accurately and categorised correctly so that conservation efforts are appropriately allocated. In Australia, the emballonurid Saccolaimus saccolaimus is currently listed as ‘Critically Endangered’. On the basis of new observations and existing museum specimens, we used a combination of genetic (mitochondrial DNA sequence) and morphological (pelage characteristics, dig III : phalanx I length ratio, inter-upper canine distance) analyses to identify six new geographic records for S. saccolaimus, comprising ~100 individuals. Our analyses also suggested that there are likely to be more records in museum collections misidentified as S. flaviventris specimens. The external morphological similarities to S. flaviventris were addressed and genetic, morphological and echolocation analyses were used in an attempt to provide diagnostic characters that can be used to readily identify the two species in the field. We recommend genetic testing of all museum specimens of Australian Saccolaimus to clarify species’ distributions and provide data for reassessing the conservation status for both S. saccolaimus and S. flaviventris. Museum curators, taxonomists and wildlife managers need to be aware of potential species misidentifications, both in the field and laboratory. Misidentifications that result in misclassification of both threatened and non-threatened species can have significant implications.

scholarly journals Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains

Revista CERES ◽  
2019 ◽  
Vol 66 (1) ◽  
pp. 72-76
Author(s):  
Roberta Oliveira Viana ◽  
Karina Teixeira Magalhães-Guedes ◽  
Disney Ribeiro Dias ◽  
Rosane Freitas Schwan
Keyword(s):  
Microbial Population ◽  
Luria Bertani ◽  
Starter Cultures ◽  
Accurate Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Microbiological Control ◽  
Identification Of Species ◽  
Kefir Grains ◽  
Tof Ms

ABSTRACT The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by Maldi-Tof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.

PODISUS NIGRISPINUS (DALLAS) AND PODISUS SAGITTA (FABRICIUS) (HETEROPTERA: PENTATOMIDAE): CORRECTION OF A MISIDENTIFICATION

1995 ◽  
Vol 127 (2) ◽  
pp. 265-266 ◽  
Author(s):  
P. De Clercq ◽  
D. Degheele
Keyword(s):  
The Netherlands ◽  
New World ◽  
Stink Bug ◽  
Accurate Identification ◽  
World Species ◽  
Podisus Sagitta ◽  
Identification Of Species ◽  
Heteroptera Pentatomidae ◽  
First Time

De Clercq and Degheele (1990, 1992) described the morphology and bionomics of a predatory stink bug under the name of Podisus sagitta (Fabricius). The studies were based on a culture started from specimens originating from Surinam. Workers from the Agricultural University of Wageningen, The Netherlands, who provided us with the specimens, referred to the species by the name “Podisus sagittus (L.)”(Adidharma 1986), which we corrected to P. sagitta (Fabricius), the name mentioned by Van Duzee (1916). Subsequent to our studies, Thomas (1992) published an illustrated key to the New World species belonging to the pentatomid subfamily Asopinae, which, for the first time, enabled accurate identification of species.

scholarly journals Cross-genera amplification and identification of Colpodella sp. with Cryptosporidium primers in fecal samples of zoo felids from northeast China

2023 ◽  
Vol 83 ◽  
Author(s):  
S. Hussain ◽  
X. Li ◽  
S. M. Bukhari ◽  
M. Zhou ◽  
S. Ahmad ◽  
...  
Keyword(s):  
Northeast China ◽  
Diarrheal Disease ◽  
Clinical Epidemiology ◽  
Domestic Animals ◽  
Human Pathogens ◽  
Specific Primers ◽  
Accurate Identification ◽  
Fecal Samples ◽  
Identification Of Species ◽  
Water Borne

Abstract The protozoans include many intracellular human pathogens. Accurate detection of these pathogens is necessary to treat the diseases. In clinical epidemiology, molecular identification of protozoan is considered a more reliable and rapid method for identification than microscopy. Among these protozoans, Cryptosporidium considered being one of the important water-borne zoonotic pathogens and a major cause of a diarrheal disease named cryptosporidiosis in humans, domestic animals, and wild animals. This study was aimed to identify Cryptosporidium in zoo felids (N= 56) belonging to different zoo of China, but accidentlly Colpodella was encountered in the zoo felids sample and phylogenetic data confirmed this unexpected amplification from fecal samples using two-step nested-PCR. Phylogenetic analysis revealed the fact about the specific primers used previously by many researchers and cross-genera amplification. We came to know that genetically sequenced amplicon gives more accurate identification of species. This study suggests more investigation on Colpodella which has been neglected previously but gains the attention of researchers after identified from humans and animals and has been known to correlate with neurological symptoms in patients.

First DNA sequence reference library for mammals and plants of the Eastern Mediterranean Region

Genome ◽  
2020 ◽  
pp. 1-11
Author(s):  
Liliane Boukhdoud ◽  
Carole Saliba ◽  
Lillian D. Parker ◽  
Nancy Rotzel McInerney ◽  
Ghiwa Ishak Mouawad ◽  
...  
Keyword(s):  
Plant Species ◽  
Mediterranean Region ◽  
Eastern Mediterranean ◽  
Eastern Mediterranean Region ◽  
12S Rrna ◽  
Reference Library ◽  
Mammal Species ◽  
Accurate Identification ◽  
Identification Of Species ◽  
Conservation Projects

The Mediterranean region is identified as one of the world’s 36 biodiversity hotspots, with the Earth’s most biologically rich yet threatened areas. Lebanon is a hub for Eastern Mediterranean Region (EMR) biodiversity with 9116 characterized plant and animal species (4486 fauna and 4630 flora). Using DNA barcoding as a tool has become crucial in the accurate identification of species in multiple contexts. It can also complement species morphological descriptions, which will add to our understanding of the biodiversity and richness of ecosystems and benefit conservation projects for endangered and endemic species. In this study, we create the first reference library of standard DNA markers for mammals and plants in the EMR, with a focus on endemic and endangered species. Plant leaves were collected from different nature reserves in Mount Lebanon, and mammal samples were obtained from taxidermized museum specimens or road kills. We generated the 12S rRNA sequences of 18 mammal species from 6 orders and 13 different families. We also obtained the trnL and rbcL barcode sequences of 52 plant species from 24 different families. Twenty-five plant species and two mammal species included in this study were sequenced for the first time using these markers.

Polydnavirus gene provides accurate identification of species in the genusHyposoter(Hymenoptera: Ichneumonidae)

2015 ◽  
Vol 8 (4) ◽  
pp. 348-358
Author(s):  
Victoria G. Pook ◽  
Eric G. Chapman ◽  
Daniel H. Janzen ◽  
Winnie Hallwachs ◽  
M. Alex Smith ◽  
...  
Keyword(s):  
Accurate Identification ◽  
Identification Of Species

scholarly journals Diagnosis and Population Analysis of Pythium Species Using AFLP Fingerprinting

Plant Disease ◽  
2005 ◽  
Vol 89 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Carla D. Garzón ◽  
David M. Geiser ◽  
Gary W. Moorman
Keyword(s):  
Control Strategies ◽  
Population Analysis ◽  
Rapid Identification ◽  
Molecular Techniques ◽  
Pythium Species ◽  
Aflp Fingerprinting ◽  
Accurate Identification ◽  
Pythium Root Rot ◽  
Identification Of Species

Accurate identification of Pythium species, the causal agents of Pythium root rot and dampingoff of seedlings, and characterization of populations within the species would greatly assist in selecting and implementing control strategies for these pathogens. Several molecular techniques offer methods for accurate and rapid identification of species, but provide little information about their populations. In this study, amplified fragment length polymorphism (AFLP) fingerprinting was used to characterize plant-pathogenic Pythium species and intraspecific populations. Species-diagnostic AFLP fingerprints for Pythium aphanidermatum, P. irregulare, and P. ultimum, and tentative fingerprints for six other species, were identified. Intraspecific distance analyses of P. aphanidermatum, P. ultimum, and P. irregulare revealed distinct patterns of intraspecific variation among the three species. P. aphanidermatum showed the smallest mean distance among isolates (15%), followed by P. ultimum (37%). P. irregulare had the largest mean distance among isolates (64%), which were divided into two populations with great genetic differentiation (FST = 0.2), suggesting the presence of a cryptic species boundary within this species.

scholarly journals Alternaria diseases of agricultural crops in Uzbekistan

2020 ◽  
Vol 13 (2) ◽  
pp. 062-067
Author(s):  
Gulchehra Kulmuminovna Khalmuminova ◽  
Shukhrat Ganievich Kamilov ◽  
Olga Antonovna Verushkina ◽  
Mutabar Kushakovna Khujanazarova
Keyword(s):  
Species Composition ◽  
Preliminary Data ◽  
Agricultural Crops ◽  
Vegetable Crops ◽  
Biochemical Traits ◽  
Accurate Identification ◽  
Crop Species ◽  
Wide Range ◽  
Pure Cultures ◽  
Identification Of Species

In the article the tasks have been set on the inventory of the species composition of fungi of the genus Alternaria Nees ex Walroth - pathogen of various Alternaria diseases of vegetable crops in Uzbekistan. More than 200 isolates were collected and about 150 monosporous pure cultures were obtained from them. According to preliminary data, they belong to 15 species of the genus Alternaria and have been isolated from the 12 most important crop species. It was found that a more accurate identification of species requires the study of a wide range of morphological and biochemical traits. The article discusses the issue of infection of vegetable seeds in Uzbekistan. The composition of micromycetes living on seeds of vegetable and green crops was revealed. In total, 21 species were recorded, of which 9 species includes to Alternaria genus.

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